L0066

The murine αTC1 cell line was obtained from ATCC (Cat#CRL-2934, RRID:CVCL_B036). Cells were grown in low-glucose DMEM medium (Biowest L0066) supplemented with 10% FBS, 50 U/mL penicillin and 50 μg/mL streptomycin. HAP1 cells (Horizon discovery) were grown in IMDM medium (Sigma I6529) supplemented with 10% FBS, 50 U/mL penicillin and 50 μg/mL streptomycin. The Lenti-X™ 293 T cell line was purchased from Takara Bio (632180). Cells were grown in high-glucose DMEM medium (Sigma D5796) supplemented with 10% FBS, 1 mM sodium pyruvate, 50 U/mL penicillin and 50 μg/mL streptomycin.

The human cervical cancer cell line HeLa was purchased from the American Type Culture Collection (CCL-2, ATCC). The spontaneously immortalized human keratinocyte cell line HaCaT, used as non-tumorigenic keratinocytes, was donated by Ph.D. Efrain Garrido along with C-33 A. HeLa and HaCaT cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) low glucose (L0066, Biowest, Nuaillé, France) and C-33 A in DMEM-F12 (L0092, Biowest), all supplemented with 10% fetal bovine serum (FBS) (S1650, Biowest) and penicillin–streptomycin (L0022, Biowest) 100 U/mL. The cell lines were incubated at 37 °C in a humidified atmosphere with 5% CO₂. The HPV status in both cell lines was evaluated using PCR with the universal oligonucleotides MY09/MY11 and was only detected in HeLa (Supplementary Figure S1).