Data are expressed as means ± standard deviation for n = 6. Graphs were plotted in Microsoft Office Excel 2017 and Significance was calculated by Student’s t-test using Graphpad Prism software. A P- value of <0.05 was considered significant.
Office excel 2017
Manufactured by GraphPad
Office Excel 2017 is a spreadsheet software application developed by Microsoft. It is primarily used for organizing, analyzing, and visualizing data. Excel provides a grid-based interface for inputting, calculating, and formatting data.
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Lab products found in correlation
4 protocols using office excel 2017
1
Optimized Cell Culture Conditions
2
Annexin V-APC and PI Apoptosis Assay
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After PBS washing, cells were stained with Annexin V-APC Apoptosis Kit (BD Pharmingen™ Cat no 556,547) and propidium iodide. Stained cells, acquired by FACS Canto (Becton Dickinson, San Jose, USA) flow cytometry were analyzed by Diva Software 6.3. For each experimental point, 30.000 total events were acquired. The percentage of early, late apoptotic, and necrotic cells was analyzed using Microsoft Office Excel 2017 and GraphPad Prism 7.0 software. Apoptosis was also quantified with the “Cell Death detection ELISA kit” (Roche).
3
Statistical Analysis of Experimental Data
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Statistical significance was determined by one-way ANOVA with Tukey post hoc test within groups following normal distribution or Kruskal–Wallis with Dunn’s post hoc test in case of not normally distributed values. Differences between treated samples and control cells with p ≤ 0.05 were assumed to be statistically significant. The results were analyzed with the Microsoft Office Excel 2017 and GraphPad Prism 7.0 software.
4
Cellular Analyses with Statistical Rigor
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The experiments were performed in three replicates. The results are reported as mean ± standard deviation. The significance of the differences between the mean values of different groups of cells were assessed via one-way and two-way ANOVA with the Tukey post hoc test. Differences between treated samples and control cells that reached p ≤ 0.05 were assumed to be statistically significant. The results were analyzed using Microsoft Office Excel 2017 and GraphPad Prism 8.0 software.
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