Pantropic vsvg

Manufactured by Addgene

Pantropic VSVg is a plasmid that encodes the glycoprotein (G) from the Vesicular Stomatitis Virus (VSV). The VSVg protein facilitates the entry of viral particles into a wide range of cell types, making it a widely used component in lentiviral and retroviral vector systems for gene delivery.

Automatically generated - may contain errors

Lab products found in correlation

Polyethylenimine (pei), by Merck Group (2 mentions) Pspax2, by Addgene (2 mentions) Puromycin dihydrochloride, by Corning (2 mentions) Blasticidin, by InvivoGen (2 mentions) 0.45 μm filter, by Thermo Fisher Scientific (1 mentions) 0.45 m filter, by Thermo Fisher Scientific (1 mentions)

2 protocols using pantropic vsvg

Generating Pseudotyped Lentiviral and Retroviral Vectors

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Vesicular stomatitis virus (VSV)-G protein pseudotyped lentiviruses and retroviruses were prepared by using polyethylenimine (PEI; Sigma) to co-transfect 293T cells with a lentiviral or retroviral plasmid and pantropic VSVg (Addgene) and either psPAX2 (Addgene) (lentivirus) or pCL-Eco (retrovirus) packaging plasmids. After culture in HMEC-1 medium for 48 hrs at 37°C, the viral supernatant was harvested, filtered through a 0.45 μm filter (Thermo Fisher Scientific), and used immediately. Cells stably expressing pHAGE-xCT-HA-FLAG were selected in medium containing 1 μg/mL puromycin dihydrochloride (Corning), and cells expressing pMXS-SLC1A3 were selected in 5 μg/mL blasticidin (Invivogen).

Cohen E.B., Geck R.C, & Toker A. (2020). Metabolic pathway alterations in microvascular endothelial cells in response to hypoxia. PLoS ONE, 15(7), e0232072.

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Lentiviral and Retroviral Production

Check if the same lab product or an alternative is used in the 5 most similar protocols

Vesicular stomatitis virus (VSV)-G protein pseudotyped lentiviruses and retroviruses were prepared by using polyethylenimine (PEI; Sigma) to co-transfect 293T cells with a lentiviral or retroviral plasmid and pantropic VSVg (Addgene) and either psPAX2 (Addgene) (lentivirus) or pCL-Eco (retrovirus) packaging plasmids. After culture in HMEC-1 medium for 48 hrs at 37°C, the viral supernatant was harvested, filtered through a 0.45 µm filter (Thermo Fisher Scientific), and used immediately. Cells stably expressing pHAGE-xCT-HA-FLAG were selected in medium containing 1 μg/mL puromycin dihydrochloride (Corning), and cells expressing pMXS-SLC1A3
were selected in 5 μg/mL blasticidin (Invivogen).

Cohen E.B., Geck R.C., & Toker A. (2020). Metabolic pathway alterations in microvascular endothelial cells in response to hypoxia.

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