ARQ 092 and ARQ 087 were synthesized at ArQule, Incorperated. Cycletest plus DNA reagent kit was purchased from BD Biosciences (Franklin Lakes, New Jersey, USA). Primary antibodies for western blot analysis were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA): phospho-AKT1 (Ser473) (Cat# 9271), pan-AKT (Cat# 2920), phospho-ERK (Thr202/Tyr204) (Cat# 9101), total ERK (Cat# 9107), phospho-S6 ribosomal protein (Ser235/236) (Cat# 4858), phospho-S6 ribosomal protein (S240/244) (Cat# 2215), phospho-GSK-3β(S9) (Cat#9336), phospho-FoxO1 (T24)/3a(T32) (Cat# 9464), phospho-Bad (S136) (Cat# 4366), and total FGFR2 (Cat# 11835). Phospho-FGFR1–4 (Y653/Y654) was purchased from R&D systems (Minneapolis, Minnesota, USA) and β-actin (Cat# A2228) from Sigma-Aldrich (St Louis, Missouri, USA). Precast Tris Glycine gels and PVDF were purchased from Life Technologies (Carlsbad, California, USA).
Phospho bad s136
Manufactured by Cell Signaling Technology
Sourced in United States
Phospho-Bad (S136) is a primary antibody product developed by Cell Signaling Technology. It is designed to detect the phosphorylation of the Bad protein at serine 136.
Automatically generated - may contain errors
Lab products found in correlation
Pan akt, by Cell Signaling Technology (1 mentions)
Phospho akt1 ser473, by Cell Signaling Technology (1 mentions)
Phospho gsk3β s9, by Cell Signaling Technology (1 mentions)
Polyvinylidene fluoride (pvdf), by Thermo Fisher Scientific (1 mentions)
Pre cast tris glycine gel, by Thermo Fisher Scientific (1 mentions)
Total erk, by Cell Signaling Technology (1 mentions)
Phospho s6 ribosomal protein ser235 236, by Cell Signaling Technology (1 mentions)
Phospho erk thr202 tyr204, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Cycletest plus dna reagent kit, by BD (1 mentions)
Phospho rps6 s235 236, by Cell Signaling Technology (1 mentions)
Total s6k1, by Cell Signaling Technology (1 mentions)
Phospho foxo3a, by Cell Signaling Technology (1 mentions)
Total pras40, by Cell Signaling Technology (1 mentions)
Phospho acc s79, by Cell Signaling Technology (1 mentions)
Phospho 4e bp1 s65, by Cell Signaling Technology (1 mentions)
Total foxo3a, by Cell Signaling Technology (1 mentions)
Phospho pras40 t246, by Cell Signaling Technology (1 mentions)
Phospho s6k1 t389, by Cell Signaling Technology (1 mentions)
Total rps6, by Cell Signaling Technology (1 mentions)
2 protocols using phospho bad s136
1
Comprehensive Western Blot Analysis
2
Western Blot Analysis of Cellular Proteins
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Cells were lysed in cell lysis buffer (20 mM Tris, pH 7.5, 135 mM NaCl, 5% [v/v] glycerol, 50 mM NaF, 0.1% [v/v] Triton X-100, plus protease inhibitors), centrifuged and protein quantified using Bradford reagent (Sigma-Aldrich). Samples were made up in NuPAGE LDS sample buffer (Life Technologies). Western blotting was performed as previously described [28 (link)]. Blots shown are representative of 3 independent experiments. Anti-β-actin (#4967), phospho-TSC2 S1387 (#5584), total TSC2 (#3990), IRE1α (3294S), phospho-S6K1 T389 (#9205), total S6K1 (#9202), phospho-rpS6 S235/236 (#2211), total rpS6 (#2217), phospho-4E-BP1 S65 (#9451), total 4E-BP1 (#9644), phospho-ACC S79 (#3661), total ACC (#3676), PARP (#9542), caspase 3 (#9662), ATF4 (#11815), phospho-FOXO3a (#9466S), total FOXO3a (#9467), phospho-PRAS40 T246 (#2997), total PRAS40 (#2691) and phospho-Bad S136 (#4366) antibodies were from Cell Signaling Technology (Danvers, MA, USA). GADD34 antibody (10449-1-AP) was from Proteintech (Manchester, UK).
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