The DAS-ELISA was performed to quantify the BNYVV accumulation in the samples using the Bioreba AG Kit (Catalog No. # 160175). The DAS-ELISA assay was carried out according to the manufacturer’s recommendations. Bio-Rad Novapath Microplate Reader was used to measure absorbance values of the samples at 405 nm wavelength. The data were analyzed following the Meunier47 (link) procedure. The DAS-ELISA test was carried out separately for each replicate and average values were calculated (Table 1 ).
Novapath microplate reader
Manufactured by Bio-Rad
The Novapath Microplate Reader is a laboratory instrument designed to measure the absorbance of samples in microplates. It is capable of performing various photometric assays, including enzyme-linked immunosorbent assays (ELISA), cell-based assays, and other absorbance-based experiments.
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Lab products found in correlation
2 protocols using novapath microplate reader
1
Quantifying BNYVV Accumulation via DAS-ELISA
2
Cell viability and cytotoxicity assay
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Cells were seeded in a 96-multiwell at a density of 10 000 cells per well. After 24 h, correctors dissolved in 1‰ DMSO were added at the indicated concentrations. After 24 h of treatment, cell viability was determined measuring the bioreduction of MTS (Owen’s reagent) by living cells with the Cell Titer 96 Aqueous proliferation assay (Promega) according to the manufacturer’s instruction. Cell toxicity was determined measuring the release of LDH in culture supernatant with the Cytotox 96 cytotoxicity assay (Promega) according to the manufacturer’s instruction. A maximum LDH release control was performed adding a lysis solution 45 min before adding Cytotox 96 reagent, and LDH release was calculated as (experimental LDH release-blank)/(maximum LDH release-blank)×100.
All spectrophotometric measurements were performed with a Novapath Microplate Reader (Bio-Rad). All experiments were run in sextuplicate.
All spectrophotometric measurements were performed with a Novapath Microplate Reader (Bio-Rad). All experiments were run in sextuplicate.
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