Pre-erythrocytic and hypnozoite stages: ACP-immunolabeled parasites were quantified considering the distinguishable morphology in drug-treated versus control by an individual with the aid of confocal (Leica SP8 white laser microscope) at 63X magnification. For each condition, 100 parasites were observed. Erythrocytic stage: TPT-HA–immunolabeled parasites were quantified considering the distinguishable morphology in drug-treated versus control by an individual with the aid of fluorescence microscope (Axio Imager 2_apotome; ZEISS, ×100 magnification). For each condition, between 54 and 116 parasites were observed.
Sp8 white laser microscope
Manufactured by Leica
Sourced in France
The Leica SP8 white laser microscope is a high-performance imaging system designed for advanced research applications. It features a white light laser source that provides a broad spectrum of excitation wavelengths, allowing for flexible and versatile fluorescence imaging. The SP8 combines exceptional optical performance with advanced hardware and software capabilities to deliver reliable and accurate results.
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Lab products found in correlation
3 protocols using sp8 white laser microscope
1
Quantification of Drug-Induced Parasite Stages
2
Quantifying Nuclei in Egg Chambers
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Confocal z-stacks of whole egg chambers were collected on a Leica SP8 white laser microscope. Each egg chamber was divided in three regions. Nuclei were counted twice per region.
3
Analyzing Plasmodium Apicoplast Confocal Imaging
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Confocal images of immunostained cultures were taken by Leica SP8 white laser microscope controlled by the Leica Application Suite AF software at the QUANT microscopy platform (Institut du Cerveau et de la Moelle épinière, ICM) in Paris, France. Z-stack images were constructed and analyzed by Fiji package of ImageJ software. Final images were made by Microsoft Powerpoint. Schematic figures of Plasmodium apicoplast were designed by Procreate application in Apple ipad pro.
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