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Gelatin coated black walled 96 well plates

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Gelatin-coated black-walled 96-well plates are a type of laboratory equipment designed for various experiments. The plates feature a black-colored wall and are coated with gelatin, a protein-based substance. The 96-well format provides a standardized arrangement for conducting multiple tests or experiments simultaneously.

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Lab products found in correlation

Hoechst dye, by Thermo Fisher Scientific (2 mentions) Elisa reader, by Promega (2 mentions) Tnf α, by Thermo Fisher Scientific (2 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Black-walled 96-well plates Gelatin-coated 96-well plates 96-well black plates Gelatin-coated plates 96-well plates Gelatin

2 protocols using gelatin coated black walled 96 well plates

1

HUVEC-THP-1 Adhesion Assay

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HUVECs were transfected 48 h before the assay and transferred to gelatin-coated black-walled 96-well plates (Falcon) and stimulated with TNF-α (10 ng/ml; Peprotech) or PBS (Gibco) for 24 h. Directly before start of the assay, THP-1 cells [32 ] were stained with Hoechst dye (Thermo Fisher) and added to the HUVECs after stringent washing. Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C, 5% CO2. Cells were washed with PBS and fluorescence was measured again.
Gimbel A.T., Koziarek S., Theodorou K., Schulz J.F., Stanicek L., Kremer V., Ali T., Günther S., Kumar S., Jo H., Hübner N., Maegdefessel L., Dimmeler S., van Heesch S, & Boon R.A. (2022). Aging-regulated TUG1 is dispensable for endothelial cell function. PLoS ONE, 17(9), e0265160.
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2

HUVEC-THP-1 Cell Adhesion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
HUVECs were transfected 48 h before the assay and transferred to gelatin-coated blackwalled 96-well plates (Falcon) and stimulated with TNF-α (10 ng/ml; Peprotech) or PBS (Gibco) for 24 h. Directly before start of the assay, THP-1 cells (32) were stained with Hoechst dye (Thermo Fisher) and added to the HUVECs after stringent washing. Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C, 5% CO 2 . Cells were washed with PBS and fluorescence was measured again.
Gimbel A.T., Koziarek S., Theodorou K., Schulz J.F., Stanicek L., Ali T., Günther S., Kumar S., Jo H., Hübner N., Mäegdefessel L., Dimmeler S., Heesch S.v., & Boon R.A. (2022). Aging-regulated TUG1 is dispensable for endothelial cell function.
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