Silymarin (Legalon 140™) was obtained from Rottapharm-Madaus Co. (Cairo, Egypt). Corn oil was gotten from the united oil processing and packing company S.A.E (El-Sharkya, Egypt). CCl4 (purity grade, 99.9%) was obtained from Sigma-Aldrich (St Louis, MO, USA). Kits of Thiobarbituric acid reactive substances (TBARS), nitric oxide (NO), glutathione (GSH), glutathione-S-reductase (GSR), glutathione-S-transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD), Serum total protein (TP), alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-c) were obtained from Biodiagnostic (Cairo, Egypt). Irisin kit was purchased from Cono Biotech Co. Ltd. (China). Biozol component was bought from Invitrogen (CA, USA). Primers for NF-κB, COX-2, IL-6, TNF-α, and the transforming growth factor beta (TGF-β) were obtained from Bioneer (Korea). SYBER Green 1-step Kit was obtained from Thermo Scientific (USA).
Glutathione peroxidase
Manufactured by Biodiagnostic
Sourced in Egypt
Glutathione peroxidase is an enzyme that catalyzes the reduction of hydrogen peroxide and other organic hydroperoxides to water and corresponding alcohols. It plays a crucial role in the antioxidant defense system of cells by protecting them from oxidative damage.
Automatically generated - may contain errors
Lab products found in correlation
Superoxide dismutase, by Biodiagnostic (3 mentions)
Creatinine, by Biodiagnostic (2 mentions)
Uric acid, by Biodiagnostic (2 mentions)
Catalase, by Biodiagnostic (2 mentions)
Reduced glutathione, by Biodiagnostic (2 mentions)
Glutathione s transferase, by Biodiagnostic (1 mentions)
Tnf α, by Bioneer (1 mentions)
Interleukin 6 (il 6), by Bioneer (1 mentions)
Total protein, by Biodiagnostic (1 mentions)
Glutathione s transferase gst, by Biodiagnostic (1 mentions)
Ab263883, by Abcam (1 mentions)
Alkaline phosphatase, by Biodiagnostic (1 mentions)
Nefa kit, by Fujifilm (1 mentions)
Malondialdehyde, by Biodiagnostic (1 mentions)
Total antioxidant capacity, by Biodiagnostic (1 mentions)
6 protocols using glutathione peroxidase
1
Silymarin Protects Against CCl4-Induced Liver Injury
2
Biochemical Assays for Oxidative Stress
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All kits used for biochemical studies such as catalase (EC 1.11.1.6), superoxide dismutase (EC 1.15.1.1), glutathione peroxidase (EC 1.11.1.9), glutathione reduced, lipid peroxidation, total protein, uric acid and creatinine were purchased from Biodiagnostic Company, Dokki, Giza, Egypt. All other chemicals were of reagent grades and obtained from Sigma-Aldrich and local scientific distributors in Egypt.
3
Fipronil Biochemical Assays in Toxicology
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Fipronil (Insecto SC 5%) is a product of BASF Company and manufactured by, Sinochem Group Ningbo Technical Co., Ltd., China. The assay kits used for biochemical measurements of aspartate aminotransferases (AST, EC 2.6.1.1.), alanine aminotransferases (ALT, EC 2.6.1.2), alkaline phosphatase (ALP, EC 3.1.3.1), lactate dehydrogenase (LDH, EC 1.1.1.27), superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), glutathione peroxidase (GPx, EC 1.11.1.9), glutathione-s-transferase (GST, EC 2.5.1.13), glutathione reduced (GSH), lipid peroxidation (LPO), albumin, uric acid and creatinine were purchased from Biodiagnostic Company, Dokki, Giza, Egypt. All other chemicals were of reagent grades and were obtained from reputed companies.
4
Doxorubicin-Induced Cardiotoxicity: Evaluation Protocol
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DOX was obtained as an injectable commercial product (Adriblastina vials, Pharmacia Italia S.P.A., Italy). Each vial has 50 mg DOX hydrochloride (dried powder) and kept at 4°C. Before use, each vial's contents were dissolved in 25 ml of sterile saline solution so each ml contains 2 mg DOX.
HES (powder form) was obtained from Sigma Aldrich (St. Louis, MO, USA), kept at 2°C–4°C and freshly dissolved in distilled water just before use.
Kits for Superoxide dismutase (SOD), Catalase (CAT), and Glutathione peroxidase (GPX) were purchased from Biodiagnostic Company, Giza, Egypt.
In addition, Cardiac markers assessment Kits; Troponin I (CTnI) ELISA Kits were obtained from IMMUNOSPEC Corporation, USA, CAT. No. E29-061. Creatine Kinase Activity (CK) (ab155901), Creatine Kinase MB (CK-MB) (ab285275), and Aspartate Aminotransferase (AST) (ab263883) kits were purchased from Abcam, UK. Lactate dehydrogenase enzyme (LDH) kits were purchased from the Egyptian Company of Biotechnology, EGYPT.
ELISA Kits for the exact measurement of Interleukin 1β (IL-1β) Cat. No. E0094Hu, Tumor necrosis factor α (TNF-α) Cat. No. E0082Hu and Interferon γ (IFN-γ) Cat. No. E0105Hu were purchased from Bioassay Technology Laboratory Company, China.
HES (powder form) was obtained from Sigma Aldrich (St. Louis, MO, USA), kept at 2°C–4°C and freshly dissolved in distilled water just before use.
Kits for Superoxide dismutase (SOD), Catalase (CAT), and Glutathione peroxidase (GPX) were purchased from Biodiagnostic Company, Giza, Egypt.
In addition, Cardiac markers assessment Kits; Troponin I (CTnI) ELISA Kits were obtained from IMMUNOSPEC Corporation, USA, CAT. No. E29-061. Creatine Kinase Activity (CK) (ab155901), Creatine Kinase MB (CK-MB) (ab285275), and Aspartate Aminotransferase (AST) (ab263883) kits were purchased from Abcam, UK. Lactate dehydrogenase enzyme (LDH) kits were purchased from the Egyptian Company of Biotechnology, EGYPT.
ELISA Kits for the exact measurement of Interleukin 1β (IL-1β) Cat. No. E0094Hu, Tumor necrosis factor α (TNF-α) Cat. No. E0082Hu and Interferon γ (IFN-γ) Cat. No. E0105Hu were purchased from Bioassay Technology Laboratory Company, China.
5
Biochemical Profiling of Serum Markers
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Serum biochemical concentrations were measured using commercial test kits in accordance with standard protocols. The serum amyliod A (SAA) and plasma fibrinogen (Fb) concentrations were assessed using IBL International Crop (Canada)® ELISA kits, and haptoglobin (Hp) by Eagle Biosciences (Columbia) ELISA kits. The total protein, albumin, glucose, cholesterol, urea, and creatinine were determined by kits from Gamma Trade Company, Egypt. The insulin levels were measured by kits from NeoBiolab, Cambridge, USA. For T3 and T4 commercial test, Malondialdehyde (MDA), catalase (CAT), reduced glutathione (GSH), super oxide dismutase (SOD), Glutathione peroxidase (GPx) and alkaline phosphatase (ALP) were provided by Bio diagnostic, Egypt. Betahydroxybutyrate (BHBA) provided by Cayman chemical Company, USA. NEFA kits from FUJIFILM Wako Chemicals, USA. The IGF1 kits from Wuhan Fine Biotech. Co, China. Total bilirubin from Atlas Medical, Germany. Globulin was calculated by subtracting albumin values from total serum protein.
6
Antioxidant Activity Evaluation
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Sodium fluoride (NaF; ≥99%; MW 41.99; EC No. 231-667-8) was purchased from Sigma-Aldrich (Hamburg, Germany). Kits for malondialdehyde (Cat# MD 2529), Superoxide dismutase (Cat# SD 2521), catalase (Cat# CA2517), glutathione peroxidase (Cat# GP 2524), glutathione reduced (Cat# GR 2511), and total antioxidant capacity (Cat# TA 25 13) were obtained from Biodiagnostic Co., Cairo, Egypt. All chemicals were of the highest available analytical grades.
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