Benzalkonium chloride

Manufactured by MP Biomedicals

Sourced in United States, France

Benzalkonium chloride is a quaternary ammonium compound that functions as a cationic surfactant and antimicrobial agent. It is commonly used in various laboratory applications as a disinfectant and preservative.

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Lab products found in correlation

Ethanol, by Merck Group (2 mentions) Hydrogen peroxide, by Merck Group (2 mentions) Glutaraldehyde, by Avantor (2 mentions) Peracetic acid, by Merck Group (2 mentions) Sodium hypochlorite, by Merck Group (2 mentions) Chlorhexidine, by Merck Group (2 mentions) Povidone iodine, by Merck Group (2 mentions) Chlorophene, by Merck Group (2 mentions) Chloroform d, by Merck Group (1 mentions) Linoleic acid, by Fujifilm (1 mentions) Wakogel c 300 hg, by Fujifilm (1 mentions) 1 1 diphenyl 2 picrylhydrazyl, by Tokyo Chemical Industry (1 mentions) β carotene, by Fujifilm (1 mentions) Gam broth, by Nissui Pharmaceutical (1 mentions) Protocatechualdehyde, by Fujifilm (1 mentions) Gifu anaerobic medium broth, by Nissui Pharmaceutical (1 mentions) Protocatechuic acid, by Fujifilm (1 mentions) Vanillin, by Fujifilm (1 mentions)

4 protocols using benzalkonium chloride

Biocide Solutions Preparation Protocol

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Benzalkonium chloride (MP Biomedicals), hydrogen peroxide (Macron), peracetic acid (Sigma-Aldrich), sodium hypochlorite (Sigma-Aldrich), glutaraldehyde (Amresco), chlorhexidine (Aldrich), chlorhexidine gluconate (Spectrum), and povidone-iodine (Sigma) stock solutions were prepared by dilution in sterile, demineralized water. Chlorophene (Aldrich) stock solution was prepared by dilution in ethanol (Sigma-Aldrich). To refer to each biocide, we use the abbreviations provided in Table 1. All solutions, as well as ethanol (Sigma-Aldrich) and isopropanol (Spectrum), were sterilized with 0.22-μm-pore-size filters and kept in the dark at 8°C. Working solutions were prepared daily by further dilutions in sterile, demineralized water.

Merchel Piovesan Pereira B., Wang X, & Tagkopoulos I. (2020). Short- and Long-Term Transcriptomic Responses of Escherichia coli to Biocides: a Systems Analysis. Applied and Environmental Microbiology, 86(14), e00708-20.

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Palladium-Activated Carbon Antioxidant Assay

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Palladium-activated carbon (10% Pd-C), Wakogel C-300HG, butylated hydroxytoluene (BHT), linoleic acid, Tween 40, β-carotene, methyl linoleate, and miconazole nitrate were obtained from Wako Pure Chemical Industries., Ltd. (Osaka, Japan). Cellulose powder was obtained from Toyo Roshi Kaisha., Ltd. (Tokyo, Japan). Chloroform-d [99.8% atom% D, containing 0.03% (v/v) TMS] was obtained from Sigma-Aldrich (Tokyo, Japan). 1,1-Diphenyl-2-picrylhydrazyl was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). GAM broth was obtained from Nissui Pharmaceutical Co., Ltd. (Tokyo, Japan). Benzalkonium chloride was obtained from MP Biomedicals, LLC (Santa Ana, CA, USA). Peptone was obtained from Nihon Pharmaceutical Co., Ltd. (Tokyo, Japan).

Tsujimura M., Goto M., Tsuji M., Yamaji Y., Ashitani T., Kimura K., Ohira T, & Kofujita H. (2019). Isolation of diterpenoids from sugi wood-drying byproducts and their bioactivities. J Wood Sci., 65(1).

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Detailed Microbial Culture Protocol

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Difco TM Nutrient broth and agar were purchased from Becton, Dickinson & Company, France. Gifu anaerobic medium (GAM) broth and agar were purchased from Nissui Pharmaceutical Co. Ltd., Japan. Anaero Pack system was procured from Mitsubishi Gas Chemical Co. Inc., Japan. Benzalkonium chloride was obtained from MP Biomedicals, LLP, France. Sorbic acid, protocatechualdehyde, homovanillic acid, vanillin, protocatechuic acid and the organic solvents used for chemical characterization were procured from Wako Pure Chemical Industries, Japan. Hispidin was obtained from Amadis Chemicals, China. All the chemicals and solvents used were of analytical grade with purity of at least 97%.

Tamrakar S., Nishida M., Amen Y., Tran H.B., Suhara H., Fukami K., Parajuli G.P, & Shimizu K. (2017). Antibacterial activity of Nepalese wild mushrooms against Staphylococcus aureus and Propionibacterium acnes. J Wood Sci., 63(4).

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Disinfectant solutions preparation

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Benzalkonium chloride (MP Biomedicals), hydrogen peroxide (Macron), peracetic acid (Sigma-Aldrich), sodium hypochlorite (Sigma-Aldrich), glutaraldehyde (Amresco), chlorhexidine (Aldrich), chlorhexidine gluconate (Spectrum), and povidone-iodine (Sigma) stock solutions were prepared by dilution in sterile, demineralized water. Chlorophene (Aldrich) stock solution was prepared by dilution in ethanol (Sigma-Aldrich). All solutions, including ethanol (Sigma-Aldrich) and isopropanol (Spectrum), were sterilized with 0.22 μm filters and kept at 4°C. Working solutions were prepared daily by further dilutions in sterile, demineralized water.

Merchel Piovesan Pereira B., Wang X, & Tagkopoulos I. (2021). Biocide-Induced Emergence of Antibiotic Resistance in Escherichia coli. Frontiers in Microbiology, 12, 640923.

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