C57BL/6, Tyrc-2J/c-2J (B6-Albino), and CXCL12flox/flox mice were purchased from the Jackson Laboratory. CXCL12DsRed mouse was the gift from Dr. Sean Morrison (29 (link)). OT-I TCR transgenic mouse was crossed with C57BL/6-Tg(CAG-EGFP)1Osb/J (Jackson Laboratory) for EGFP-expressing OT-1 strain (OT-1GFP). CXCL12flox/flox was crossed with elastase2 (Ela)Cre/+ for granulocyte-specific knock-out of CXCL12 and CXCL12flox/floxElaCre/+ mice were used for experiments with their littermates CXCL12flox/floxEla+/+ or ElaCre/+ as a control group. Genotyping for each strain was performed according to the corresponding reference (29 (link)). All mice were maintained in a pathogen-free environment of the University of Rochester animal facility and the animal experiments were approved by the University Committee on Animal Resources at the University of Rochester (Rochester, NY, USA).
Cxcl12flox flox
Manufactured by Jackson ImmunoResearch
CXCL12flox/flox is a genetic construct that contains loxP sites flanking the Cxcl12 gene. This allows for cell-specific or temporal deletion of the Cxcl12 gene through Cre-mediated recombination.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6 tg cag egfp 1osb j, by Jackson ImmunoResearch (1 mentions)
C57bl 6, by Jackson ImmunoResearch (1 mentions)
R26mt mg, by Jackson ImmunoResearch (1 mentions)
Tie2 cre, by Jackson ImmunoResearch (1 mentions)
R26 tdtomato, by Jackson ImmunoResearch (1 mentions)
Lgr5 dtr gfp mice, by Genentech (1 mentions)
Lgr5 egfp ires creert, by Jackson ImmunoResearch (1 mentions)
R26 lacz, by Jackson ImmunoResearch (1 mentions)
Sox2egfp, by Jackson ImmunoResearch (1 mentions)
2 protocols using cxcl12flox flox
1
Genetically Modified Mice for Research
2
Transgenic Mouse Models in Cancer Research
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Mist1-CreERT mice [36 (link)] and Cxcl12-dsRED mice [37 (link)] were described previously. Cxcr4-EGFP mice were provided from Richard J. Miller (North-western University Medical School). LSL-KrasG12D and LSL-Trp53R172H mice were provided by Dr. Kenneth Olive (Columbia University). Cckbr-GFP BAC transgenic mice were purchased from MMRRC (GENSAT project [38 (link)]). Lgr5-DTR-GFP mice were provided by Genentech. Apcflox/flox mice were obtained from the National Cancer Institute. Lgr5-EGFP-IRES-CreERT, Sox2-EGFP, R26-mTmG, R26-TdTomato, R26-LacZ, Cxcl12flox/flox, and Tie2-Cre mice were purchased from the Jackson Laboratory. FucciG1 mice were obtained from RIKEN BRC. Cre recombinase was activated by oral administration of tamoxifen (TAM, Sigma, 3 mg/0.2 ml corn oil). All animal studies and procedures were approved by the ethics committees at Columbia University and the University of Tokyo. All mice were bred under specific pathogen free conditions. Comparisons were made with age- and sex- matched control animals.
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