Trypsin, streptomycin and penicillin, RPMI-1640 medium (containing L-glutamine and high glucose), and fetal bovine serum (FBS) were prepared from Gibco (Grand Island, NY, USA). Chemotherapeutic agents (daunorubicin and mitoxantrone), thiazolyl blue tetrazolium bromide (MTT), Tween-20, phosphate-buffered saline tablet (PBS), bovine serum albumin (BSA), bavachinin, candidone, tephrosin, and MDR pump inhibitors (novobiocin sodium salt and verapamil hydrochloride) were bought from Sigma-Aldrich (Deisenhofen, Germany). P-gp and ABCG2 primary antibodies (mouse anti-P glycoprotein and anti-BCRP/ABCG2 monoclonal IgG, Abcam), anti-mouse secondary IgG1 of goat and FITC-conjugated IgG2a, and the suitable isotype controls were prepared from Abcam (Cambridge, USA). DMSO (dimethyl sulfoxide) was used to prepare the stocks of flavonoids and drugs, but to prepare different concentrations at the time of the experiment, a whole culture medium was used. DMSO, methanol, formaldehyde, and the other analytical-grade chemicals and solvents were obtained by Merck (Darmstadt, Germany).
Phosphate buffered saline tablet pbs
Manufactured by Merck Group
Sourced in Germany, United States
Phosphate-buffered saline (PBS) tablet is a laboratory reagent used to prepare a buffered saline solution. It contains a mixture of salts that help maintain a stable pH environment for various biological applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Verapamil hydrochloride, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
Thiazolyl blue tetrazolium bromide, by Merck Group (1 mentions)
Daunorubicin, by Merck Group (1 mentions)
Mitoxantrone, by Merck Group (1 mentions)
Novobiocin sodium salt, by Merck Group (1 mentions)
Span 60, by Merck Group (1 mentions)
Polyethylene glycol peg 4000, by Merck Group (1 mentions)
3 4 dihydroxyphenylalanine l dopa, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
3 protocols using phosphate buffered saline tablet pbs
1
Evaluating Multidrug Resistance in Cancer Cells
2
Cytotoxicity and Antimicrobial Evaluation
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Gallic acid (purity ≥ 95%), sorbitan monostearate (SpanTM 60), polyoxyethylene sorbitan monolaurate (Tween® 60), 2,2-diphenyl-1-picryl-hydrazine-hydrate (DPPH˙) reagent, 3-(4, 5-dimethyl-2-thiazolyl)-2, dimethyl sulfoxide (DMSO), 5-diphenyl-2H-tetrazolium bromide (MTT), phosphate-buffered saline tablet (PBS) (dissolved in deionized water), L-3,4-dihydroxyphenylalanine (DOPA), gentamycin, penicillin, and streptomycin were obtained from Sigma-Aldrich (St. Louis, MO, USA); cholesterol (purity, 95%), polyethylene glycol (PEG) 4000, and other chemicals were purchased from Merck (Darmstadt, Germany); B16F10 cell lines were provided by the Pasteur Institute Cell Bank (Tehran, Iran); fetal bovine serum, and Dulbecco’s Modified Eagle Medium (DMEM) were purchased from GIBCO (Billings, MT, USA); three strains of Gram-negative—Klebsiella pneumoniae (ATCC 10031), Pseudomonas aeruginosa (ATCC 27853), and Escherichia coli (ATCC 11333)—were obtained from the Iranian Biological Resource Center.
3
Sprague-Dawley Rat Husbandry Protocol
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In this study, chemical materials including HES (CAS registry number: 520-26-3) and phosphate-buffered saline tablet (PBS) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Healthy adult male Sprague–Dawley rats were purchased from the Center for Comparative and Experimental Medicine, Shiraz University of Medical Sciences (SUMS), Shiraz, Iran. The rats weighed around 220 ± 5 g and were housed in accordance with particular practices described in “The Guide for The Care and Use of Laboratory Animals” arranged by SUMS in the university animal house. The practices involved some specific measures which include as follows: characteristics of animal natural life in captivity situation, using spacious cages, preparing appropriate ventilation and light, handling with care, giving standard pellet diet and water ad libitum, etc. All the animals were housed in certain circumstances including temperature (23 ± 2°C), humidity (55% ±5%), and light (12 h of light and dark cycle). Besides, four animals were separately housed together in polypropylene cages containing sterile husk bedding during the experiments, and this study was conducted based on the instructions issued by the SUMS’ Ethical Committee.
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