Ne 680 fast

Manufactured by PerkinElmer

Sourced in United Kingdom

The NE 680 fast is a high-performance liquid chromatography (HPLC) system designed for rapid analysis. It features a modular design and is capable of delivering fast separation and high-resolution results.

Automatically generated - may contain errors

Lab products found in correlation

Ivis spectrum, by PerkinElmer (1 mentions) Ivis spectrum system, by PerkinElmer (1 mentions) Living image 3, by PerkinElmer (1 mentions) Mmpsense 680, by PerkinElmer (1 mentions) Molecular imaging software, by Bruker (1 mentions)

4 protocols using ne 680 fast

Neutrophil Infiltration in Ischemic Myocardium

Check if the same lab product or an alternative is used in the 5 most similar protocols

Detection of neutrophils into the ischemic region of the heart was performed on OCT‐embedded heart tissue sections immunostained for collagen IV, endothelial cells and neutrophils. For localization of neutrophils into the cremaster muscles and mesenteric tissue following I/R injury, whole‐mount tissues were fluorescently immunostained for neutrophils, the endothelium and the perivascular BM. For NE expression, tissues were fluorescently immunostained for neutrophils, NE and the perivascular BM. For NE activity, the NE‐fluorescent activatable substrate NE680FAST (Perkin Elmer, Beaconsfield, UK) was injected i.v. prior to reperfusion. All samples were viewed using laser scanning confocal microscopes and images were analyzed using IMARIS software. The area and intensity of matrix protein LERs within the perivascular BM were measured using ImageJ (NIH & Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI, USA).

Voisin M., Leoni G., Woodfin A., Loumagne L., Patel N.S., Di Paola R., Cuzzocrea S., Thiemermann C., Perretti M, & Nourshargh S. (2019). Neutrophil elastase plays a non‐redundant role in remodeling the venular basement membrane and neutrophil diapedesis post‐ischemia/reperfusion injury. The Journal of Pathology, 248(1), 88-102.

+ Open protocol

+ Expand

In Vivo NE Imaging in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

At the experimental end-point, mice were placed on the alfalfa-free diet (Teklad global 16% protein, Envigo) for two-weeks prior to imaging. Mice received 4nmols of a NE-selective optical probe (NE 680 fast; PerkinElmer) in 0.1 mL PBS via tail vein injection. 16h post probe injection, activity was measured using imaging system IVIS Spectrum (PerkinElmer) or in excised tumors using fluorescent microscopy. Signal intensity was imaged and quantified using ImageJ (v1.49).

Taya M., Garcia-Hernandez M.D., Rangel-Moreno J., Minor B., Gibbons E, & Hammes S.R. (2020). Neutrophil Elastase from Myeloid Cells Promotes TSC2-null Tumor Growth. Endocrine-related cancer, 27(4), 261-274.

+ Open protocol

+ Expand

In Vivo MMP and Neutrophil Elastase Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

MMP and neutrophil elastase (NE) activities were measured using enzyme-selective optical probes (MMPSense 680 and NE 680 fast; PerkinElmer). Thirty-week-old mice were injected with 100 µL (4 nmols) of probe via tail vein. In vivo imaging by the IVIS Spectrum system (PerkinElmer) was performed after 24 hours. Images were processed using Living Image 3.2 software (PerkinElmer). Ex vivo activity measurements were performed in excised uteri using fluorescent microscopy and intensity was analyzed using ImageJ (NIH).

Prizant H., Taya M., Lerman I., Light A., Sen A., Mitra S., Foster T.H, & Hammes S.R. (2016). Estrogen maintains myometrial tumors in a lymphangioleiomyomatosis model. Endocrine-related cancer, 23(4), 265-280.

+ Open protocol

+ Expand

Thoracic Fluorescence Imaging in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse thorax fur was removed prior to fluorescence imaging analyses. NE 680 FAST (NEV11169, PerkinElmer) was used as an imaging agent and administered (100 µL) via tail vein injection. Mice were anesthetized with an inhalation anesthetic (isoflurane) and placed under the in vivo X-treme imaging system to detect fluorescent signals. Image analyses were performed with molecular imaging software (Bruker). The region of interest (ROI) was drawn in the thorax, and the fluorescence intensity within the ROI was calculated.

Yang H.J., Tsou W.H., Shen M.C., Liu C.Y., Saunders H.M., Wang K.Y, & Douglas F.L. (2022). The effects of hydrogen treatment in a cigarette smoke solution-induced chronic obstructive pulmonary disease-like changes in an animal model. Journal of Thoracic Disease, 14(11), 4246-4255.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!