Primary microglia were separated and purified from rat cortices as described before [40 (link)]. The microglia were acquired from the astrocytic single layer by beating the flasks 10 to 20 times and planted to poly-d-lysine-coated culture plates until used. In 150-cm3 culture flasks, 5 107 cells were seeded with DMEM containing 10% heat-inactivated FBS, 2 mM l-glutamine, 1 mM sodium pyruvate, 100 mM non-essential amino acids, 50 U/ml penicillin, and 50 g/ml streptomycin. Microglial cells were exposed to 100 ng/ml lipopolysaccharide (LPS; SigmaAldrich) and cultured with 5 or 10 μg/ml ADSCs-Exo for 24 h, so as to evaluate the effect of ADSCs-Exo on LPS-stimulated primary microglial cells. Cells processed without either LPS or exosomes were set as controls. LPS (L3012), l-glutamine (G2150), sodium pyruvate (P2256), and non-essential amino acids (TMS-001) were from Sigma.
Tms 001
Manufactured by Merck Group
The TMS-001 is a laboratory instrument designed for the analysis of various chemical and biological samples. It utilizes a specialized technique to measure and detect specific analytes within the samples. The core function of the TMS-001 is to provide accurate and reliable data to support research and analytical activities in a laboratory setting.
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Lab products found in correlation
2 protocols using tms 001
1
LPS-Stimulated Microglial Modulation by ADSC Exosomes
2
Immortalized Bovine Intestinal Epithelial Cells
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The primary BIEC were isolated and cultured completely as described previously (Zhan et al., 2017) (link). The primary BIEC were then immortalized, and BIEC, a cell line, were used in this study (Zhan et al., 2020) (link). The cells were seeded and cultured in DMEM/F12 complete medium (11330057, Gibco) supplemented with 10% fetal bovine serum (10099141, Gibco), 100 U/mL penicillin and 100 μg/mL streptomycin (10378016, Gibco), 4 mM glutamine (G8540, Sigma-Aldrich), 15 ng/mL epidermal growth factor (E4127, Sigma-Aldrich), 1 μg/mL hydrocortisone (H0888, Sigma-Aldrich), and 1% NEAA [containing glycine, l-alanine, l-asparagine, l-aspartic acid, l-glutamic acid, l-proline, and l-serine, 10 mM each (TMS-001, Sigma-Aldrich)] and seeded on 10-cm 2 plastic dishes (351092, Corning). Cells were incubated at 37°C in a humidified atmosphere containing 5% CO 2 , and the medium was exchanged every 24 h. When the confluence was between 85% and 95%, cells were pas-saged routinely. The BIEC purified in 20-25 passages were used for experimental assays. In this study, every experiment was repeated three times, and within each date, three wells were performed in each treatment.
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