Tofacitinib; hydrocortisone, an internal standard (IS) used for the quantification of Tofacitinib; and P-407 were obtained from Sigma-Aldrich (St. Louis, MO, USA). A nicotinamide adenine dinucleotide phosphate hydrogen (NADPH)-generating system was obtained from Corning Inc. (Corning, NY, USA). β-Cyclodextrin was from Wako (Osaka, Japan); high-performance liquid chromatography (HPLC)-grade ethyl acetate and acetonitrile were from J.T. Baker (Phillipsburg, NJ, USA); and injectable 0.9% NaCl and heparin were from JW Pharmaceutical Co. (Seoul, Republic of Korea). Antibodies against CYP3A1/2, CYP2C11, CYP2D1, CYP2B1/2, and CYP1A1/2 were kindly donated by Detroit R&D Inc. (Detroit, MI, USA), and primary antibody against P-gp was purchased from Abcam (Cambridge, UK). β-Actin was obtained from Cell Signaling Technology (Beverly, MA, USA), and secondary goat, rabbit, and mouse antibodies were from Bio-Rad (Hercules, CA, USA). Other chemicals and solvents were of HPLC or analytical grade and were used without further purification.
Primary antibody against p gp
Manufactured by Abcam
Sourced in United States
The primary antibody against P-gp is a laboratory reagent used to detect and quantify the expression of the P-glycoprotein (P-gp) protein. P-gp is a membrane transport protein involved in the efflux of various molecules from cells. This antibody can be used in techniques like Western blotting, immunohistochemistry, and flow cytometry to study the expression and localization of P-gp in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Tofacitinib, by Merck Group (1 mentions)
β cyclodextrin, by Fujifilm (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Ecl plus detection system, by GE Healthcare (1 mentions)
Polyvinylidene fluoride pvdf membrane, by Bio-Rad (1 mentions)
Protein extraction kit, by Keygen Biotech (1 mentions)
2 protocols using primary antibody against p gp
1
Tofacitinib Quantification in ADME Studies
2
Photodynamic Therapy for Multidrug-Resistant Breast Cancer
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MDA-MB-231/ADR cells were incubated with RGD-NPVs@MNPs/DOX. After 4 h of incubation, the cells were irradiated with or without the NIR laser (1.5 W cm-2) for 10 min. At 4 h post-irradiation, the cells were washed and collected. The total cell protein was extracted using a protein extraction kit (KEYGEN, China). The protein concentration was determined with a BCA protein assay. The extracted proteins were separated using 12% SDS-PAGE electrophoresis and transferred onto polyvinylidene fluoride (PVDF) membranes (Bio-Rad). After a 1 h blocking step with 5% skim milk, the membrane was incubated with the primary antibody against P-gp (Abcam) overnight at 4 °C, and then the membrane was incubated with the secondary antibody for 1 h at room temperature. The resulting bands were visualized using an ECL-plus detection system (GE Healthcare).
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