Rag1 deficient

Manufactured by Jackson ImmunoResearch

RAG1-deficient is a lab equipment product. It is a genetically modified organism that lacks the RAG1 gene, which is essential for the initiation of V(D)J recombination, a process that generates the diversity of antigen receptors in lymphocytes. The core function of RAG1-deficient is to provide a controlled system for studying the role of RAG1 in immune system development and function.

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Lab products found in correlation

C57bl 6, by Jackson ImmunoResearch (2 mentions) Foxp3 mrfp mice, by Jackson ImmunoResearch (1 mentions) Stinggt, by Jackson ImmunoResearch (1 mentions) Neomycin, by Merck Group (1 mentions) Metronidazole, by Merck Group (1 mentions) Ampicillin, by Merck Group (1 mentions)

3 protocols using rag1 deficient

Mouse Strain Acquisition for Immunological Research

Cited 2 times

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C57BL/6, RAG1-deficient and Foxp3-mRFP mice were purchased from The
Jackson Laboratory. Id3-GFP mice were a gift from Ananda Goldrath (UCSD, La
Jolla, California) and have been previously described (12 (link), 14 (link)). Mice
were bred and housed under the approval of the Institutional Animal Care and Use
Committee of the Benaroya Research Institute.

Sullivan J.M., Höllbacher B, & Campbell D.J. (2018). Dynamic expression of Id3 defines the stepwise differentiation of tissue-resident regulatory T cells. Journal of immunology (Baltimore, Md. : 1950), 202(1), 31-36.

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Mice Strains for Immunology Research

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C57BL/6 (RRID: IMSR_JAX:000664), CD45.1 (RRID: IMSR_JAX:002014), Rag1 deficient (RRID: IMSR_JAX:002216), OT-I (RRID:IMSR_JAX:003831), and STING^Gt (RRID: IMSR_JAX:017537) mice were all purchased from the Jackson Laboratory. Animals were bred and housed in a standard barrier animal facility at Northwestern University with the light cycle of 14:10, ambient temperature at 22 °C, and relative humidity range between 30-70%. Experimental animals were mixed-gender and randomly assigned to different treatment groups at 6 to 8 weeks old. Experimental and control animals were co-housed. All animal experiments were performed in full compliance with animal protocols approved by the Northwestern University Institutional Animal Care and Use Committee (IACUC).

Zhang P., Rashidi A., Zhao J., Silvers C., Wang H., Castro B., Ellingwood A., Han Y., Lopez-Rosas A., Zannikou M., Dmello C., Levine R., Xiao T., Cordero A., Sonabend A.M., Balyasnikova I.V., Lee-Chang C., Miska J, & Lesniak M.S. (2023). STING agonist-loaded, CD47/PD-L1-targeting nanoparticles potentiate antitumor immunity and radiotherapy for glioblastoma. Nature Communications, 14, 1610.

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Generation and Maintenance of Transgenic Mice

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C57BL/6 mice were purchased from CLEA Japan or Sankyo Laboratory service corporation. Mice with Fut1 or Fut2 loci replaced by the reporter gene LacZ (Fut1^LacZ/LacZ and Fut2^LacZ/LacZ mice), IL-22–deficient mice, IFN-γ–deficient mice, and IL-17a–deficient mice were generated as described previously (16 (link), 54 (link)–56 (link, link)). Rag1-deficient were purchased from the Jackson Laboratory. Antibiotic-treated mice were administered a mixture of broad-spectrum antibiotics (i.e., ampicillin [1 g/L; Sigma], vancomycin [500 mg/L; Shionogi], neomycin [1 g/L; Sigma], and metronidazole [1 g/L; Sigma]) in their drinking water for 4 wk, as previously described (57 (link)). Mice were maintained under specific, pathogen-free conditions at the Institute of Medical Science, the University of Tokyo, and all experiments were conducted in accordance with the guidelines of the Animal Care and Use Committees of the University of Tokyo. In all experiments, mice were used at 6 to 24 wk of age.

Kamioka M., Goto Y., Nakamura K., Yokoi Y., Sugimoto R., Ohira S., Kurashima Y., Umemoto S., Sato S., Kunisawa J., Takahashi Y., Domino S.E., Renauld J.C., Nakae S., Iwakura Y., Ernst P.B., Ayabe T, & Kiyono H. (2022). Intestinal commensal microbiota and cytokines regulate Fut2+ Paneth cells for gut defense. Proceedings of the National Academy of Sciences of the United States of America, 119(3), e2115230119.

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