For each analyzed marker, a minimum of 16 tissue sections from a minimum of four animals was used for statistical comparison. Immunohistochemical labeling of neuronal nuclei (NeuN), blood brain barrier stability (IgG) and astrocytes (GFAP) was performed using previously established methods47 (link),62 (link),65 (link). The following primary and secondary antibodies were used: Primary: Rabbit anti-glial fibrillary acidic protein (GFAP) (1:500, Z0334, Daco), mouse anti-neuronal nuclei (NeuN) (1:250, MAB377, Millipore), and rabbit anti-immunoglobulin G (IgG) (1:100, 618501, Bio-Rad). Secondary: Anti-mouse Alexa Fluor 488 (1:1000, A11029, LifeTechnologies) and anti-rabbit Alexa Fluor 594 (1:1000, A11037, LifeTechnologies).
A11029
The A11029 is a laboratory equipment product from Thermo Fisher Scientific. It is designed to perform a specific core function within a laboratory setting. However, without access to the technical specifications or detailed product information, I cannot provide a comprehensive and unbiased description of the product's capabilities or intended use. Therefore, the detailed description is not available.
Lab products found in correlation
255 protocols using a11029
Immunohistochemical Analysis of Brain Tissue
For each analyzed marker, a minimum of 16 tissue sections from a minimum of four animals was used for statistical comparison. Immunohistochemical labeling of neuronal nuclei (NeuN), blood brain barrier stability (IgG) and astrocytes (GFAP) was performed using previously established methods47 (link),62 (link),65 (link). The following primary and secondary antibodies were used: Primary: Rabbit anti-glial fibrillary acidic protein (GFAP) (1:500, Z0334, Daco), mouse anti-neuronal nuclei (NeuN) (1:250, MAB377, Millipore), and rabbit anti-immunoglobulin G (IgG) (1:100, 618501, Bio-Rad). Secondary: Anti-mouse Alexa Fluor 488 (1:1000, A11029, LifeTechnologies) and anti-rabbit Alexa Fluor 594 (1:1000, A11037, LifeTechnologies).
Antibody Characterization and Procurement
Immunofluorescence Staining of Cells
Immunofluorescence Protocol for Cellular Phenotyping
Phenotype Verification of ARPE-19 and HREC Cells
Immunofluorescence Microscopy of V5-tagged Proteins
Immunofluorescence Staining of m-SKPs
Immunofluorescence Analysis of CDH1 in BeWo Cells
Subcellular Localization of P-FOXO and P-ERK
Immunofluorescence Staining of Human iPSC-Derived Hepatocytes
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!