Unfertilized S. purpuratus eggs were prepared for injection as previously described [29 (link)] stuck to 35 mm petri dishes (Fisher Scientific) coated with 0.25% protamine sulfate and fertilized. One-cell zygotes were then injected at between 2–5% egg volume with the mRNA mixture described above. Ampicillin (Sigma Aldrich, St. Louis, MO) at 100 μg/mL in FSW was added to the injection plate. Embryos were then cultured at 15°C (±1°C) for between 16 and 48 hours.
35 mm petri dishes
Manufactured by Thermo Fisher Scientific
Sourced in United States, Denmark
The 35 mm Petri dishes are a standard-sized, sterile laboratory container used for the cultivation and observation of cells, tissues, or microorganisms. These dishes provide a controlled environment for culturing samples and conducting experiments.
Automatically generated - may contain errors
Lab products found in correlation
Axopatch 200a amplifier, by Molecular Devices (2 mentions)
Paraformaldehyde (pfa), by Polysciences (2 mentions)
Cover glasses, by Thermo Fisher Scientific (2 mentions)
Ampicillin, by Merck Group (1 mentions)
Esg1106, by Merck Group (1 mentions)
Cytochalasin b, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Collagenase, by Merck Group (1 mentions)
Sylgard, by Dow (1 mentions)
Dispase, by Merck Group (1 mentions)
Dmem f12 1 1, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Axio observer z1 microscope, by Zeiss (1 mentions)
Plan apochromat 60 1.40 oil dic objective, by Zeiss (1 mentions)
Ma200, by Nikon (1 mentions)
Cellmask deep red plasma membrane stain, by Thermo Fisher Scientific (1 mentions)
Petri pulser applicator, by Harvard Apparatus (1 mentions)
15 protocols using 35 mm petri dishes
1
Sea Urchin Egg Microinjection
2
Tracking Cell Behavior with Image Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Natural waves were relayed in populations of cells aggregating on a plastic surface in submerged cultures in 35 mm Petri dishes (Fisherbrand, Pittsburg, PA). Cell behavior was recorded and motion analyzed as previously described [30] (link), [66] (link), [71] (link), [72] . Cell behavior in this case was analyzed using JDIAS 4.1.
3
Photodynamic Therapy with TONS504 in EMT6 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EMT6 cells were seeded at 4–5×104 cells/well in 35-mm petri dishes (Nalge Nunc International, Penfield, NY, USA) containing 2 ml RPMI-1640 medium supplemented with 10% FBS. Following 24 h of incubation, the cells were divided into the TONS504 groups (treated with 10 or 30 µg/ml TONS504) and the PDT groups (treated with 10 or 30 µg/ml TONS504 and then irradiated with a light dose of 1, 5 or 10 J/cm2). The concentration of used TONS504 was selected by taking into consideration the results of the cytotoxicity effects of TONS504 and light on EMT6 cells.
The cells were incubated with 10 or 30 µg/ml TONS504 for 24 h. Following washing with fresh media, the cells were irradiated with a laser light of wavelength 677 nm (11 mW/cm2; 0, 1, 5 or 10 J/cm2). Following 24 h of PDT, the cells were stained using the PromoKine Apoptotic/Necrotic Cells Detection kit, according to the manufacturer's protocols. To assess apoptosis and necrosis, the cells were stained with Annexin V-fluorescein isothiocyanate (FITC) and ethidium homodimer III (EthD-III) at 24 h after laser irradiation. Cell morphology was examined using a CLSM. The cells were analyzed by fluorescence microscopy using an FITC and Texas Red filter set.
The cells were incubated with 10 or 30 µg/ml TONS504 for 24 h. Following washing with fresh media, the cells were irradiated with a laser light of wavelength 677 nm (11 mW/cm2; 0, 1, 5 or 10 J/cm2). Following 24 h of PDT, the cells were stained using the PromoKine Apoptotic/Necrotic Cells Detection kit, according to the manufacturer's protocols. To assess apoptosis and necrosis, the cells were stained with Annexin V-fluorescein isothiocyanate (FITC) and ethidium homodimer III (EthD-III) at 24 h after laser irradiation. Cell morphology was examined using a CLSM. The cells were analyzed by fluorescence microscopy using an FITC and Texas Red filter set.
4
Wnt5a Expression in Intestinal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To assess the expression of Wnt5a in ISC lysates and to determine whether Wnt5a was released into culture media, ISCs were seeded into Nunclon™ 35 mm Petri dishes. After 72 hours, ISCs from each Petri dish were trypsinised, resuspended, and supplemented with complete ULTRA mini protease inhibitors and sonicated. The ISC-conditioned media (CM) was also collected and concentrated. The control samples were composed of the ISC culture media alone, which was also concentrated ×12. Wnt5a was measured in the ISC lysates and CM using iTRAQ protein screening.
5
Mouse Embryonic Stem Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All cell culture reagents were supplied from Invitrogen unless otherwise specified. Mouse E14 (ATCC, CRL-1821) ES cells were cultured in 5% CO2/95% O2 in DMEM supplemented with 10% ES cell qualified FBS, 2 mM l -glutamax, 1 mM sodium pyruvate, 100 μM non-essential amino acid, 0.1 mM β-mercaptoethanol, 1000 U mL−1 mouse leukemia inhibitory factor (LIF, Millipore, ESG1106), and 100 U mL−1 penicillin/100 μg mL−1 streptomycin. For differentiation, 35 mm Petri dishes (Nunclon) were coated with fibronectin solution, where undifferentiated mouse ES cells were seeded at a moderate density (103 cells cm−2) in culture medium without LIF.
6
Colony Formation Assay for Breast Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A colony assay was performed as described previously [15 (link)]. Cells were counted and appropriate numbers of MDA-MB-231 and T47D cells were plated into 60- and 35-mm petri dishes (Nunc), respectively. To obtain a countable number of colonies, the number of cells plated was increased depending on the radiation dose. After incubation of MDA-MB-231 and T47D cells at 37℃ for 7 and 12 days, the colonies were fixed, stained, and counted. The surviving fraction was calculated as the number of colonies/(number of cells×control plating efficiency) in three independent experiments. A linear-quadratic model was used to fit the survival curves.
7
Cytochalasin-B Induced Micronuclei Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After incubation of the cells in the cluster for 24 h, the cells were washed twice with PBS and then sub-cultured in 35 mm Petri dishes (Nunc, Kamstrupvej, Denmark). The medium was replaced with fresh medium containing 1.5 μg/mL cytochalasin-B (Sigma-Aldrich, St. Louis, MO, USA) at 5 h after cell seeding, and the cells were cultured for a further 24 h. The cells were then fixed with 2% paraformaldehyde (Sinopharm Chmemical Reagent, Shanghai, China), and stained with DAPI (Beyotime, Shanghai, China). The MN in bi-nucleated (BN) cells were morphologically identified and more than 1000 BN cells were scored for each sample.
8
Hematopoietic Stem Cell Subpopulation Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thawed CD34+ cells were selected for their CXCR4 and CD133 expression and sorted as CXCR4negCD133−, CXCR4negCD133+, CXCR4lowCD133− and CXCR4lowCD133+ subpopulations. Day-0 sorted subfractions were expanded separately ex vivo for 7 days. Day-0 and day-7 subpopulations were plated in methylcellulose cytokine-supplemented kits “Stemα-1D” (Saint Clement les Places, France) (1000 cells/mL for each cell population) and cul tured for 14 days (37°C, 20% O2, 5% CO2) in 35 mm Petri dishes (NUNC, Roskilde, Denmark) in duplicate. The colonies (>50 cells) were scored19 (link) as burst-forming unit - erythroid (BFU-E), colony-forming unit - granulocyte and macrophage (CFU-GM) and multi-lineage colony-forming unit (CFU-mix).
9
Whole-cell patch-clamp recording of TR146 cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TR146 epithelial cells were grown in 35 mm petri dishes (Nunc) for 48 h before recordings at low cell density (10-30% confluence). Cells were superfused with a modified Krebs solution (120 mM NaCl, 3 mM KCl, 2.5 mM CaCl2, 1.2 mM MgCl2, 22.6 mM NaHCO2, 11.1 mM glucose, 5 mM HEPES pH 7.4). Isolated cells were recorded at room temperature (21-23°C) in whole cell mode using microelectrodes (5-7 MΩ) containing 90 mM potassium acetate, 20 mM KCl, 40 mM HEPES, 3 mM EGTA, 3 mM MgCl2, 1 mM CaCl2 (free Ca2+ 40 nM), pH 7.4. Cells were voltage clamped at -60 mV using an Axopatch 200A amplifier (Axon Instruments) and current/voltage curves were generated by 1 s steps between -100 to + 50 mV. Treatments were applied to the superfusate to produce the final required concentration, with vehicle controls similarly applied. Data was recorded using Clampex software (PClamp 6, Axon Instrument) and analyzed with Clampfit 10.
10
Isolation and Culture of Insect Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult houseflies were anaesthetised with CO2 and placed on ice. Flies were pinned through the abdomen onto Sylgard (Dow-Corning) coated dishes, decapitated and the thorax dissected along the dorsal midline. Thoracic ganglia were removed and maintained in Ca 2+ /Mg 2+ -free Rinaldini's saline (in mM; 135 NaCl, 2.5 KCl, 0.4 NaH2PO4, 1.25 NaHCO3, 0.5 Glucose, 5.0 HEPES, pH 7.2). Connective tissue was removed from each ganglion and the neural sheath disrupted mechanically prior to treatment with 0.5 mg ml -1 collagenase (Sigma) and 2 mg ml -1 dispase (Sigma) in Ca 2+ / Mg 2+ -free Rinaldini's saline for 1 hour at room temperature. Ganglia were washed several times with Ca 2+ /Mg 2+ -free Rinaldini's saline and transferred to modified Schneider's culture medium (85% Schneider's Drosophila medium, 15% Foetal Bovine Serum, plus 100 units ml -1 penicillin and 100 μg ml -1 streptomyocin). Ganglia were gently triturated through the flame polished tip of a Pasteur pipette to liberate neurons into the culture media, and the supernatant was plated directly onto 35 mm Petri dishes (Nunc, Roskilde). Dishes were left overnight at 18 o C to allow neurons to settle and stick to the surface of the dish.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!