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135 protocols using contour glucometer

1

Assessing Glucose and Insulin Metabolism in Mice

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For IPGTT, baseline blood glucose and body weights were measured after overnight fasting with free access to drinking water. Mice were i.p. injected with 2.0 g/kg body weight D-glucose and blood glucose levels were measured at 30, 60, 90, and 120 min post injection using a Bayer Contour® glucometer.
For IPITT, baseline blood glucose and body weights were measured after 4 hours of fasting with free access to drinking water. Mice were i.p. injected with 0.75U/kg body weight insulin and blood glucose levels were measured at 30, 60, 90, and 120 min post injection using a Bayer Contour® glucometer.
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2

Glucose and Insulin Tolerance Tests in Mice

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Glucose tolerance tests (GTT) were performed on 2 month old male mice in clean cages subjected to starvation with free access to water for 6 hours. Mice were weighed and a small amount of blood was obtained from tail vein for baseline (time 0) glucose measurement. Mice were then injected intraperitoneally with 50% sterile dextrose (1 mg/g body weight). Tail blood glucose was determined at 15, 30, 60, 90 and 120 min after challenge using a Bayer Contour glucometer. For insulin tolerance test (ITT), 2 month old male mice were placed in clean cages without food and free access to water. Following a 6 hr fast, the mice were weighed and baseline glucose reading was taken using Bayer Contour glucometer. Mice were injected intraperitoneally with human insulin (Humulin, Eli-Lilly) at a dose of 0.7U/Kg body weight and blood glucose measured at 15, 30, 45, 60, 90, 120 min after insulin injection.
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3

Assessing Glucose and Insulin Metabolism in Mice

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For IPGTT, baseline blood glucose and body weights were measured after overnight fasting with free access to drinking water. Mice were i.p. injected with 2.0 g/kg body weight D-glucose and blood glucose levels were measured at 30, 60, 90, and 120 min post injection using a Bayer Contour® glucometer.
For IPITT, baseline blood glucose and body weights were measured after 4 hours of fasting with free access to drinking water. Mice were i.p. injected with 0.75U/kg body weight insulin and blood glucose levels were measured at 30, 60, 90, and 120 min post injection using a Bayer Contour® glucometer.
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4

Metabolic Tolerance Tests in Mice

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Blood glucose was measured using a Contour glucometer (Bayer). For intraperitoneal glucose tolerance tests (IPGTT), overnight fasted (13 h) mice were injected with D-glucose solution at 2 g/kg. For insulin tolerance tests (ITT), animals were injected with 0.75 U/kg body weight of a 5 × 10−2 U/ml insulin solution after a 6-h fasting period. For intraperitoneal pyruvate tolerance test (PTT), mice were injected with 2 g/kg in overnight fasted mice. Blood glucose was measured using a Contour glucometer (Bayer), insulin was measured by ELISA (Chrystal Chem), and glucagon levels were determined by EIA (Phoenix Pharmaceuticals). Streptozotocin was prepared in 100 mM sodium citrate pH 4.5 at a concentration of 7.5 mg/ml and administered once i.p. in 5-h fasted mice at a dose of 150 mg/kg.
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5

Glucose Tolerance and Insulin Sensitivity

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At 6 weeks after ethanol exposure, we performed oral glucose tolerance tests (OGTTs) and insulin tolerance tests (ITTs). For the OGTT, mice were fasted overnight and gavaged with glucose (i.g. injection of a 20% solution, 1 mg/g body weight), and blood glucose was measured using a Bayer Contour glucometer after 0, 15, 30, 60, 90 and 120 min. The ITT was performed approximately 2 days after the OGTT. After fasting for 4 h and recording basal blood glucose, i.p. injection of insulin was performed at a dose of 0.75 IU/kg body weight for each mouse, and blood glucose was measured using a Bayer Contour glucometer after 15, 30, 60, 90 and 120 min.
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6

Metabolic Assessment of BPA Exposure

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At 8 weeks or 16 weeks after BPA exposure, we performed glucose tolerance tests (GTTs) and insulin tolerance tests (ITTs). For the GTT, mice were fasted overnight and injected with glucose (i.p. injection of a 20% solution, 1 mg/g body weight), and blood glucose was measured using a Bayer Contour glucometer after 0, 15, 30, 60 and 120 min. The ITT was performed approximately 2 days after the GTT. After fasting for 4 h and recording basal blood glucose, i.p. injection of insulin was performed at a dose of 0.75 IU/kg body weight for each mouse, and blood glucose was measured using a Bayer Contour glucometer after 15, 30, 60 and 120 min.
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7

Glucose Homeostasis in GKO-NSG Mice

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After a 5-hour fast (starting from 9–10 AM), mice were
administered an intraperitoneal (IP) injection of D-glucose (3 g/kg body
weight). Blood glucose levels were measured with a Contour glucometer (Bayer) at
0, 5, 15, 30, 45, 60, 90, and 120 minutes post injection and EDTA-treated plasma
samples were collected for insulin hormone assays at the same time intervals.
5–7 month-old male GKO-NSG, GKO-NSG Tx, and
NSG littermate control mice were used. For circulating
GLP-1 assessment, mice were fasted for 6 hours (starting at 9AM), then D-glucose
(6 g/ kg body weight) was given by oral gavage. Blood glucose was measured with
a Contour glucometer (Bayer) at 0, 5, 15, 30, 60, 90, 120, and 180 minutes
post-gavage and EDTA-, DPP4-inibitor- (Millipore), and HALT protease inhibitor-
(Thermo Scientific) treated plasma samples were collected at 0, 15, and 30
minutes post-gavage. 2.5–3 month-old male and female
GKO-NSG and NSG littermate control mice
were used.
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8

Glucose Homeostasis in GKO-NSG Mice

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After a 5-hour fast (starting from 9–10 AM), mice were
administered an intraperitoneal (IP) injection of D-glucose (3 g/kg body
weight). Blood glucose levels were measured with a Contour glucometer (Bayer) at
0, 5, 15, 30, 45, 60, 90, and 120 minutes post injection and EDTA-treated plasma
samples were collected for insulin hormone assays at the same time intervals.
5–7 month-old male GKO-NSG, GKO-NSG Tx, and
NSG littermate control mice were used. For circulating
GLP-1 assessment, mice were fasted for 6 hours (starting at 9AM), then D-glucose
(6 g/ kg body weight) was given by oral gavage. Blood glucose was measured with
a Contour glucometer (Bayer) at 0, 5, 15, 30, 60, 90, 120, and 180 minutes
post-gavage and EDTA-, DPP4-inibitor- (Millipore), and HALT protease inhibitor-
(Thermo Scientific) treated plasma samples were collected at 0, 15, and 30
minutes post-gavage. 2.5–3 month-old male and female
GKO-NSG and NSG littermate control mice
were used.
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9

Glucose and Insulin Tolerance Tests in Mice

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For glucose tolerance test (GTT), mice were fasted overnight (16 h) and subsequently injected (i.p.) with a bolus of a glucose solution at a dose of 1.5 g/kg body weight (BW). Blood glucose levels were measured after a tail cut at 0, 15-, 30-, 60-, and 120-min post glucose injection by using a Bayer Contour Glucometer (Bayer Healthcare, LLC.). For the insulin tolerance test (ITT), mice were intraperitoneally injected with the recombinant human insulin (Millipore) diluted in saline after a 6h fast during the light cycle at a dose of 0.75 U/kg BW. Blood glucose levels were measured as described for GTT.
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10

Glucose and Insulin Tolerance Tests in Mice

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For glucose tolerance test (GTT), the mice were fasted overnight (16 h) and then injected i.p. with glucose solution at 1.5 g/kg body weight. Blood glucose concentrations were measured using Bayer Contour Glucometer (Bayer Healthcare, LLC.) at 0, 15, 30, 60, and 120 min after glucose injection. For glucose-stimulated insulin secretion, plasma samples were collected during GTT and plasma insulin concentrations were measured (Millipore). For insulin tolerance test (ITT), the mice were fasted for 6 h during the daytime cycle, followed by an intraperitoneal injection of recombinant human insulin in saline at a dose of 0.75 U/kg body weight. Blood glucose levels were monitored at 0, 15, 30, 60 and 120 min after insulin injection.
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