The dynamic acquisition was divided into twelve 5 sec frames, four 60 sec frames, five 120 sec frames, three 5 min frames, and six 10 min scans. The data from all possible lines of response (LOR) were saved in the list mode raw data format. The raw data were then binned into 3D sinograms with a span of 3 and ring difference of 47. The images were reconstructed into transaxial slices (128 × 128 × 159) with voxel sizes of 0.0815 × 0.0815 × 0.0796 cm3, using the MAP algorithm with 16 subsets, 4 iterations, and a beta of 0.0468. For anatomical co-registration, immediately following the PET scans, the mice received a CT scan in a NanoSPECT/CT (Mediso, Washington DC) at an X-ray beam intensity of 90 mAs and x-ray peak voltage of 45 kVp. The CT images were reconstructed into 170 × 170 × 186 voxels at a voxel size of 0.4 × 0.4 × 0.4 mm3. The PET/CT images were uploaded into Amide software (www.sourceforge.com ), co-registered to an MRI template made in-house, and volumetric regions-of-interest were drawn around the cortex, hippocampus, striatum, thalamus, and cerebellum in addition to the whole brain. The PET images were normalized to the injected dose, and the time-activity-curves (TACs) of the mean activity within the ROIs were estimated for the entire duration of the scans.
Nanospect ct
Manufactured by Mediso
Sourced in Hungary, United States
The NanoSPECT/CT is a compact, high-performance small-animal imaging system that combines single-photon emission computed tomography (SPECT) and X-ray computed tomography (CT) imaging modalities. The system is designed to provide precise, high-resolution functional and anatomical data for preclinical research applications.
Automatically generated - may contain errors
Lab products found in correlation
Nucline software, by Bioscan (4 mentions)
Vivoquant post processing software, by Invicro (4 mentions)
Vivoquant, by Invicro (3 mentions)
Nucline software, by Mediso (2 mentions)
Isoflurane, by Baxter (2 mentions)
Vivoquant 2, by Invicro (1 mentions)
Wizard 1480, by PerkinElmer (1 mentions)
99mo 99mtc generator, by Mallinckrodt (1 mentions)
Teratomo software, by Mediso (1 mentions)
Vivoquant 4, by Invicro (1 mentions)
Invivoscope software, by Invicro (1 mentions)
1470 wizard, by PerkinElmer (1 mentions)
Gamma counter, by PerkinElmer (1 mentions)
Maestro 2, by PerkinElmer (1 mentions)
42 protocols using nanospect ct
1
Dynamic PET/CT Imaging of Brain Regions
2
Rat Distal Femur Defect Model
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The animal model used in the present study was a rat distal femur model. The study was approved by Chung Gung Memorial hospital (approval number: IACUC 2016092004). Four Sprague Dawley rats were used. Cylindrical defect, with the size of 3 mm in diameter and 4 mm in depth, was introduced into the distal femur by a drill. The (Ca,Sr)SO4 specimen of the same size was press fit into the defect. The recovery of these rats after surgery was normal. The rats were sacrificed 3 months postoperatively. The micro computed tomography (micro-CT, NanoSPECT/CT, MediSo Co., Hungary) of the femur was taken first. Before the histology observation, the femur was dehydrated, decalcificated, and then fixed in paraffin, cut into thin slices (thickness: 4-5 um). Masson’s trichrome (ArrayBiotech Co., Taiwan) were used as the stain.
3
In Vivo Evaluation of 177Lu-DOTANOC SPECT
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SPECT scans were performed after the injection of 177Lu-DOTANOC using a NanoSPECT/CT™ (Mediso Medical Imaging Systems, Budapest, Hungary) and Nucline Software (version 1.02, Bioscan Inc., Poway, USA). For this purpose, the energy peaks of 177Lu were set to 56.1 keV ± 10 %, 112.9 keV ± 10 %, and 208.4 keV ± 10 %. The acquired data were reconstructed using HiSPECT software (version 1.4.3049, Scivis GmbH, Göttingen, Germany). Images were prepared using the VivoQuant post-processing software (version 2.1, inviCRO Imaging Services and Software, Boston, USA). Accumulation of 177Lu-DOTANOC per volume of tumor and kidney tissue was determined using the “3D ROI” tool of the the VivoQuant post-processing software, allowing calculation of the tumor-to-kidney ratios. A Gauss post-reconstruction filter was applied for the presentation of the SPECT image, and the scale was adjusted to allow the best visualization of tumors and kidneys in which radioactivity accumulated.
4
Imaging HER2 Expression in Mice
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After subcutaneouly inoculating NCI-N87 cells (2 × 106 cells) into male BALB/c nude mice for 2 weeks, mice were received 111In-DTPA, 111In-DTPA-AHNP, 111In-DTPA-PEG, and 111In-DTPA-AHNP-PEG via intravenous injection with 1 mCi of indium-111. Nano Single-photon emission computed tomography/computed tomography (NanoSPECT/CT; Mediso Medical Imaging Systems; Budapest, Hungary) was applied to detecting HER2 distribution at 1, 4, 24, and 48 h.
5
Visualizing M. tuberculosis Lung Lesions
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A subset of live M. tuberculosis-infected animals from the relapse cohort were imaged within a sealed bio-containment bed (Minerve) modified in-house to be compliant with biosafety level 3 (BSL3) containment, as described previously [7 (link), 19 (link)]. A standard small animal anesthesia machine was used to deliver a mixture of isoflurane and oxygen to anesthetize during imaging. Computed tomography (CT) scans were then immediately performed using the NanoSPECT/CT (Mediso) in vivo animal imager. The images were analyzed using VivoQuant 2.5 (Invicro). The whole lung field was segmented and the frequency of voxels with a given density (measured as Hounsfield Units, HU) was determined. A cavity was defined as a macroscopic region of air (density <−900 HU) within diseased lung parenchyma.
6
Establishing Lung Cancer Xenografts in Mice
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Human lung cancer xenografts from NCI-H460-luc2 cells were established in Balb/c nude mice. We first performed subcutaneous implantation in order to get reference data from measurement techniques on standard conditions, so that 10 mice were anaesthetized by inhalation of 1.5% isoflurane with air (Isoflo®, AXIENCE S.A.S, France) and inoculated by different tumor burdens (either 1x105 to 2.5x106 tumor cells in 100 μL PBS) in the dorsal flank. For orthotopic implantation, 24 mice were inoculated (1.25x105 or 2.5x105 tumor cells in 25 μL PBS) using a 1.9F×50cm blunt-end silicon catheter inserted into the bronchus via a laryngoscope (S1A Fig ). This delicate procedure to get superficial cell deposition into a posterior part of a lower lobe via a main bronchus requires interventional imaging. The position of the radio-opaque catheter is checked by planar radiography (Faxitron MX20, Faxitron X-ray corp, USA) (S1B Fig ) then actual deposition of 99mTc-labelled tumor cells is controlled by Single Photon Emission Computed Tomography (Nano Spect CT, Mediso, Hungary), (S1C Fig ).
7
Imaging Nanoparticle Tumor Uptake in Mice
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1 x 106 cells were subcutaneously injected on the hind limb of C57BL6 mice and tumors were allowed to grow to volumes of 200 mm3. Tumors were either heated for one hour at 41 °C prior to injection or kept at 35 °C in a similar fashion as for the therapeutic study. 111In-TSL were i.v. injected (200 µL per mouse with an average activity of 33 ± 2 MBq 111In) and scans were made 4 h, 8 h, 24 h and 48 h after injection. Scans were acquired using the nanoSPECT/CT (Mediso Medical Imaging Systems) with the following settings for the SPECT scans: 20 projections, 60 seconds/projection, and a quality factor of 0.8. APT1 apertures were used with 1.4 mm diameter pinholes (FOV 24 + 16 mm). CT scans were acquired with 240 projections, 45 kVp tube voltage and 500 ms exposure. Data analysis was performed using InVivoScope/VivoQuant software (inviCRO, Boston, MA), where three-dimensional regions of interest were drawn over the tumor to calculate uptake of 111In-TSL at the selected time points. After the last scan, the animals were sacrificed and tumors and organs were harvested, weighed and radioactivity was determined using a γ-counter to calculate percentage injected dose per gram (%ID/g). All data were corrected for radioactive decay.
8
SPECT/CT Imaging of Folate-Targeted Radiopharmaceuticals
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The acquisition of SPECT/CT images was performed with a dedicated small-animal SPECT/CT scanner (NanoSPECT/CT™, Mediso Medical Imaging Systems, Budapest, Hungary) as previously reported (Supplementary Material) [23 (link), 25 (link)]. CT scans of 7.5 min duration time were followed by a SPECT scan of ~ 40 min of NF9006 tumor-bearing mice at 4 h and 24 h after injection of [177Lu]Lu-DOTA-folate (25 MBq, 0.5 nmol, 100 μL). During the scans, mice were anesthetized with a mixture of isoflurane and oxygen. Images were prepared using VivoQuant post-processed software (version 3.5, inviCRO Imaging Services and Software, Boston USA). A Gauss post-reconstruction filter (FWHM = 1 mm) was applied twice, and the scale of activity was indicated on the images.
9
Multiplexing Small-Animal SPECT/CT Imaging
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A small-animal, 4-head multiplexing, multipinhole camera (NanoSPECT/CT; Mediso Medical Imaging Systems, Budapest, Hungary) was employed for SPECT/CT imaging studies, as previously reported (Additional file 1 ) (Borgna et al. 2021 (link)). In brief, the SPECT scans were acquired using Nucline software (version 1.02, Mediso Ltd., Budapest, Hungary) using energy windows set at 46.0 keV (± 8.5%), 86.0 keV (± 8.5%) and 105.0 keV (± 10%). The real-time CT reconstruction used a cone-beam filtered backprojection. SPECT data were reconstructed iteratively with HiSPECT software (version 1.4.3049, Scivis GmbH, Göttingen, Germany) using standard settings. SPECT and CT data were automatically co-registered because both modalities shared the same axis of rotation. The fused datasets were processed using VivoQuant (version 3.5, inviCRO Imaging Services and Software, Boston USA). A Gaussian post-reconstruction filter (FWHM = 1.0 mm) was applied.
10
In Vivo PET-CT Imaging of Tumor Xenografts
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Mouse PET-CT imaging studies were performed as previously described in BALB/c tumor-bearing mice (16 (link)). Briefly, mice received a retro-orbital injection of approximately 37 MBq/0.1 mL 18F-FDG and were returned to plate-warmed cages. Forty minutes later, the mice were anesthetized under 2% isofluorane and imaged using the Inveon microPET (Siemens Preclinical) for 20 minutes. The raw data was then binned into 3D sinograms with a span of 3 and a ring difference of 47. The images were reconstructed into transaxial slices (128 × 128 × 159) with voxel sizes of 0.0815 × 0.0815 × 0.0796 cm3 using the MAP algorithm with 16 subsets, 4 iterations, and a beta of 0.0468. For anatomical coregistration, immediately following the PET scans, the mice underwent a CT scan using NanoSPECT/CT (Mediso) at an x-ray beam intensity of 90 mAs and an x-ray peak voltage of 45 kVp. The CT images were reconstructed into 170 × 170 × 186 voxels at a voxel size of 0.4 × 0.4 × 0.4 mm3. The PET-CT images were uploaded into Amide, and volumetric regions of interest were drawn around the tumors. The PET images were normalized to the injected dose, and the mean radiotracer concentration within the ROIs was determined.
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