Rp 18 column

Manufactured by Phenomenex

Sourced in United States

The RP-18 column is a reversed-phase chromatography column used for the separation and analysis of a wide range of compounds. It features a chemically bonded C18 stationary phase, which allows for the retention and separation of both polar and non-polar molecules. The RP-18 column provides reliable and consistent performance for a variety of analytical applications.

Automatically generated - may contain errors

Lab products found in correlation

Lcq advantage mass spectrometer, by Thermo Fisher Scientific (1 mentions) Semi preparative hplc, by Agilent Technologies (1 mentions) Breeze 2, by Waters Corporation (1 mentions) 600 controller, by Waters Corporation (1 mentions) Chlorogenic acid, by Merck Group (1 mentions) Rutin, by Merck Group (1 mentions) Prostar 335, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Luna C-18 RP column (7 citations) Gemini RP-18 column (6 citations) RP-18 (5 citations) Kinetex RP-18 column (4 citations) Synergi Fusion RP-18 column (3 citations) Hydro-RP C-18 column (2 citations) Oligo-RP C-18 reverse phase Column (2 citations) Gemini-NX RP-18 column (2 citations) Fusion RP−18 column (2 citations)

7 protocols using rp 18 column

HPLC Purity Verification of Lead Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

To verify the
purity of the lead compounds (T1, B1, S1, and S2),
of the other two hits from the tethered docking (T2 and T3), and of
the control compound (AX20017), we performed high-performance liquid
chromatography (HPLC) experiments with an HPLC HP series 1100 instrument
and an RP-18 column (Phenomenex, description: sphereClone 5 μm
ODS(2), size: 250 × 4.60 mm 5 μm). The injection volume
was 20 μL, the UV wavelength for detection was 254 nm, and the
mobile phase was the mixture of water/methanol 40:60 for all compounds,
except for compound S2 (water/methanol 10:90). The HPLC traces are
provided in the Supporting Information (Figures S15–S21). Purity of the lead compounds T1, B1, S1, and
S2 and the control compound AX20017 was confirmed to be >95%. The
purity values of two non-lead compounds (T2 and T3) were >96% and
>84%, respectively.

Burastero O., Defelipe L.A., Gola G., Tateosian N.L., Lopez E.D., Martinena C.B., Arcon J.P., Traian M.D., Wetzler D.E., Bento I., Barril X., Ramirez J., Marti M.A., Garcia-Alai M.M, & Turjanski A.G. (2022). Cosolvent Sites-Based Discovery of Mycobacterium Tuberculosis Protein Kinase G Inhibitors. Journal of Medicinal Chemistry, 65(14), 9691-9705.

+ Open protocol

+ Expand

Automated Peptide Synthesis and Radiolabeling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Automated peptide synthesis was performed with a multiple peptide synthesizer (Syro, MultiSynTech, Bochum, Germany). Preparative and semi-preparative HPLC were performed using a Shimadzu system on a Phenomenex RP18-column (21.4 × 250 mm; 10 μm/90 Å). Analytical HPLC were performed using a Merck-Hitachi system with a Phenomenex Jupiter 4u Proteo 90 Å (250 × 4.6 mm; 4 μm; 90 Å). All peptides were analyzed by matrix-assisted laser desorption ionization–time-of-flight (MALDI-ToF) using a Bruker Daltonics Ultraflex III mass spectrometer. The 1.85 - GBq (50 - mCi) ⁶⁸Ge/⁶⁸Ga-generator was purchased from iThemba Laboratories with the ⁶⁸Ge on a SnO₂-cartrige and eluted according to the manufacturer’s recommendations using a remote-controlled module.

Bergmann R., Chollet C., Els-Heindl S., Ullrich M., Berndt N., Pietzsch J., Máthé D., Bachmann M, & Beck-Sickinger A.G. (2021). Development of a ghrelin receptor inverse agonist for positron emission tomography. Oncotarget, 12(5), 450-474.

+ Open protocol

+ Expand

Isolation and Identification of Bioactive Compounds from Glycyrrhiza uralensis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The grinded G. uralensis (1 kg) was extracted using 95% ethanol solution for 24 h prior to evaporation. After evaporation, the extract was applied to a process-scale liquid column chromatography system (Isolera Flash Puri, Biotage, Uppsala, Sweden). The separation was performed using a C18 column (KP-C18-HS column, Biotage), and the solvent gradient was 95% EtOH in H₂O, and the extract was separated into 10 fractions under UV 245 nm. The major components of G9 were purified and isolated by using semi-preparative HPLC (Agilent, Palo Alto, CA, USA) on a RP-18 column (250 × 10 mm, Phenomenex, Torrance, CA, USA) at a flow rate of 1.0 mL/min, detected at UV 254 nm, 280 nm, and 300 nm. The chemical structures of the three compounds were determined through spectroscopic analysis. Electrospray ionization mass spectrometry data were acquired using an LCQ Advantage mass spectrometer (Thermo Finnigan, San Jose, CA, USA), while nuclear magnetic resonance spectra were recorded using Avance 500 and 300 MHz FT nuclear magnetic resonance spectrometers (Bruker, Bremen, Germany) at 500 MHz (¹H) and 75 MHz (¹³C). These three major compounds of G9 were identified as gancaonin G, cudraflavone C, and licoisoflavone A by comparing their NMR, MS data, and optical rotations with those reported previously [26 (link),27 (link),28 (link)].

Ou B.R., Hsu M.H., Haung L.Y., Lin C.J., Kuo L.L., Tsai Y.T., Chang Y.C., Lin W.Y., Huang T.C., Wu Y.C., Yeh J.Y, & Liang Y.C. (2023). Systematic Myostatin Expression Screening Platform for Identification and Evaluation of Myogenesis-Related Phytogenic in Pigs. Bioengineering, 10(10), 1113.

+ Open protocol

+ Expand

Biodistribution of Methotrexate in Lung Tumor

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Biodistribution studies were conducted in C57BL/6 mice induced with lung tumors (Gangjun et al., 2010 (link)). A549 cell lines (5 × 106 cells) were injected into the mice’s flank region, and tumor development was monitored for 30 days. The animals were then divided equally into three groups: Group I received NF, Group II received NFM, and Group III received Marketed MTX powder for injection. Once tumor growth in the lungs was confirmed, a single dose of PI equivalent to 4 mg/kg was reconstituted in water for injection and administered via the tail vein. After 24 h, the mice were sacrificed, and their lungs, spleens, kidneys, and hearts were excised. The excised organs were homogenized, and the amount of MTX present in each organ was analyzed (Ait Bachir et al., 2018 (link)). MTX in specific organs was extracted by vortexing in acetonitrile, followed by partitioning in a chloroform layer. The quantification of MTX was performed using HPLC with a Disodium phosphate buffer: Acetonitrile ratio of 89:11 on an Agilent 1260 Infinity Capillary LC system with a Phenomenex RP18 column (Subramaniyan et al., 2022 (link)).

Surya Teja S.P., Damodharan N., Tamilanban T., Subramaniyan V., Chitra V., Chinni S.V., Wong L.S., Fuloria N.K., Sekar M., Fuloria S., Ramachawolran G, & Selvaraj S. (2023). Impact of nanocarrier aggregation on EPR-mediated tumor targeting. Frontiers in Bioengineering and Biotechnology, 11, 1222693.

+ Open protocol

+ Expand

Quantitative Analysis of Andrographis Diterpenoids

Check if the same lab product or an alternative is used in the 5 most similar protocols

The identifications of AND, NAD, DDA were performed according to conventional approach for known structure: crystal form (Optical microscope, Shanghai Guangmi instrument Co. Ltd, in P.R. China, XSP-2C), Melting point(Yuhua instrument Co. Ltd. in P.R. China, Micro melting point apparatus X-4), IR ( PerkinElmer, American, Spectrum-100), UV (SHIMADZU,Japan, UV-2450 ) and HPLC (Waters ChemStation, Breeze2, USA).
The quantitative study of AND, NAD, and DDA in Andrographis paniculata and extracts from its processing by-products was performed by HPLC (Figure 4). The HPLC system (Waters ChemStation, Breeze2, USA) consisted of a low-pressure binary pump (model Waters 1525), a UV detector (model Waters 2489) and an autosampler (model Waters 2700 Autosampler) with a 48-vial capacity sample. The separations were carried out on a Phenomenex RP-18 column with a particle size of 250 mm (5 μm). A Phenomenex RP select B guard column with a particle size of 5 μm was placed in front of the analytical column. The chromatographic conditions were as follows: filtered (22 μm) acetonitrile and water, gradient programmed isocratic elution, room temperature, run time of 50 min, injection volume of 10 μl, and wavelength of 225 nm.Figure 4

HPLC chromatograms of three diterpenoids in Andrographis paniculata. A. Three standard diterpenoids; B. Blank sample; and C. Sample of AEE.

Hu X., Wen Y., Liu S., Luo J., Tan X., Li Z., Lu X, & Long X. (2015). Evaluation of the anaphylactoid potential of Andrographis paniculata extracts using the popliteal lymph node assay and P815 cell degranulation in vitro. Journal of Translational Medicine, 13, 121.

+ Open protocol

+ Expand

Chickpea Amino Acid Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The chickpea protein’s amino acids were identified and quantified according to the methodology described by Janssen et al. [25 (link)]. Chickpea proteins (10 mg) were hydrolyzed with 300 µL of a 6 N HCl solution at 110 °C for 24 h. The hydrolyzate obtained was derivatized with 20 µL of phenylthiocyanate (10% w/v) to generate phenylthiocarbamyl amino acids, which were separated and quantified by HPLC at 254 nm. A liquid chromatograph (Waters 600 controller, MA, USA) with a diode array detector (Waters 996) and a Phenomenex (Los Angeles, CA, USA) RP18 column (150 mm × 4.6 mm, 5 µm) was used. The gradient separation was performed using two solvent solutions: Solution (A) composed by 94:6 v/v of 0.14 mol/L of HPLC-grade anhydrous sodium acetate (pH 5.9): HPLC-grade acetonitrile, and solution (B) by HPLC-grade acetonitrile: water (60:40 v/v) solution. The injection volume was 20 µL, the column temperature was 40 °C, and the analysis time was 30 min. The quantification of amino acids was carried out with external standards (Sigma-Aldrich, Darmstadt, Germany) and attributed to 17 amino acids previously described in chickpeas [26 (link)].

Soto-Madrid D., Pérez N., Gutiérrez-Cutiño M., Matiacevich S, & Zúñiga R.N. (2022). Structural and Physicochemical Characterization of Extracted Proteins Fractions from Chickpea (Cicer arietinum L.) as a Potential Food Ingredient to Replace Ovalbumin in Foams and Emulsions. Polymers, 15(1), 110.

+ Open protocol

+ Expand

HPLC Analysis of Phenolic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Analyses were performed using HPLC Varian® Pro Star 335 with diode array detector. A Phenomenex® RP-18 column (250 x 4.6 mm, 5 μm) was used. The eluents were: (A) acetonitrile and (B) water with 0.3 % acetic acid, with the following gradient (v/v): 13 %, 0–5 min (B); 13–18 %, 5–25 min (B); 18–20 %, 25–30 min (B); 20–21 %, 30–35 min (B). Flow elution was 0.7 mL/min and 20 μL of each sample was injected. The lyophilized extract and standard solutions were resuspensed in methanol: water, 1:1 (v/v) and the final concentrations were 5 mg/mL and 50 μg/mL. The chromatogram was visualized in 270 and 340 nm, where each peak and their retention time (R_t) was compared with those of the standards. The peaks that showed similar UV and R_t were analyzed by co-injection (reference standard + extract) with the purpose of observing any increase in the peak area. Samples and solvents were previously filtered through a 0.45 μm membrane and degassed. Both rutin (purity 94 %) and chlorogenic acid (purity 95 %) were purchased from Sigma-Aldrich®, USA.

Torres-Rêgo M., Furtado A.A., Bitencourt M.A., Lima M.C., Andrade R.C., Azevedo E.P., Soares T.D., Tomaz J.C., Lopes N.P., da Silva-Júnior A.A., Zucolotto S.M, & Fernandes-Pedrosa M.D. (2016). Anti-inflammatory activity of aqueous extract and bioactive compounds identified from the fruits of Hancornia speciosa Gomes (Apocynaceae). BMC Complementary and Alternative Medicine, 16, 275.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!