Plate reading luminometer

Manufactured by Promega

Sourced in United States

The Plate-reading luminometer is a laboratory instrument used to measure luminescence from samples in a microplate format. It detects and quantifies light emitted from luminescent reactions, providing precise measurements of luminescent signals.

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Lab products found in correlation

Lactate glo assay, by Promega (1 mentions) Luciferase assay system, by Promega (1 mentions) Transfectin lipid reagent, by Bio-Rad (1 mentions) Passive lysis buffer (plb), by Promega (1 mentions) Caspase glo assay kit, by Promega (1 mentions) Solid white bottom, by BD (1 mentions) Celltiter glo, by Promega (1 mentions) Celltiter glo luminescent cell viability assay, by Promega (1 mentions)

Close spelling variants of this product

Veritas plate-reading luminometer (2 citations)

5 protocols using plate reading luminometer

Quantifying Lactate Levels in Plasma

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Lactate levels in plasma samples were quantified using the Lactate-Glo™ Assay (Promega) following the manufacturer’s instructions, using 50 μl of samples (1:100) and ten two-fold serial dilutions of a lactate standard run in duplicate in 96-well plates. Luminescence was recorded using a plate-reading luminometer (Promega). Lactate quantity was obtained extrapolating sample luminescence values from the standard curve.

Ladero-Auñon I., Molina E., Oyanguren M., Barriales D., Fuertes M., Sevilla I.A., Luo L., Arrazuria R., De Buck J., Anguita J, & Elguezabal N. (2021). Oral vaccination stimulates neutrophil functionality and exerts protection in a Mycobacterium avium subsp. paratuberculosis infection model. NPJ Vaccines, 6, 102.

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Luciferase Assay in 832/13 Cells

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Transient transfections and/or the transduction of plasmids and siRNA duplexes into 832/13 cells and cell lysis for luciferase assays were previously described [36 (link)]. Briefly, transient transfection of plasmid vectors into 832/13 cells was performed using TransFectin Lipid Reagent (BioRad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions. Cells were then lysed in 1× Passive Lysis Buffer (Promega, Madison, WI, USA) and luciferase activity was measured using the Promega Luciferase Assay System in a plate reading luminometer (Promega). Luciferase activity was normalized to total protein content as determined by the BCA assay.

Smoak P., Burke S.J., Martin T.M., Batdorf H.M., Floyd Z.E, & Collier J.J. (2022). Artemisia dracunculus L. Ethanolic Extract and an Isolated Component, DMC2, Ameliorate Inflammatory Signaling in Pancreatic β-Cells via Inhibition of p38 MAPK. Biomolecules, 12(5), 708.

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Quantifying Caspase 3/7 Activity

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Caspase 3/7 activity was measured by using a Caspase-Glo assay kit (Promega, G8091) following the manufacturer’s instructions. EGCs (10⁴ cells/well) seeded in a white tissue culture-treated 96-well plates (Falcon, solid white bottom) were treated with TcdA or TcdB alone or in the presence with 10Panx (50µM) or A438079 (300µM) for 18 h. Then, the plates containing the cells were removed from the incubator for 30 min. A volume of 100 µL of Caspase-Glo reagent was added to each well, and the wells were mixed with a plate shaker at 500 rpm for 30 s. The plates were incubated for 2 h at room temperature. Then, the luminescence of each sample was acquired in a plate-reading luminometer (Promega) to obtain the relative luminescent units (RLUs) subtracted by the background.

Loureiro A.V., Moura-Neto L.I., Martins C.S., Silva P.I., Lopes M.B., Leitão R.F., Coelho-Aguiar J.M., Moura-Neto V., Warren C.A., Costa D.V, & Brito G.A. (2022). Role of Pannexin-1-P2X7R signaling on cell death and pro-inflammatory mediator expression induced by Clostridioides difficile toxins in enteric glia. Frontiers in Immunology, 13, 956340.

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ATP Assay for Cell Viability

Cited 1 time

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Adenosine 5’-triphosphate (ATP) assays were performed to assess cell proliferation, which correlates with cell viability in monolayer cultures of osteogenically differentiated hMSCs and chondrocytes. Cell viability was measured after 10 min, 24 h and 48 h of treatment with various concentrations (no TXA, 10 mg/mL, 20 mg/mL and 50 mg/mL) of TXA using the CellTiter-Glo^® luminescent cell viability assay (Promega, Madison, WI, USA), as previously described [22 (link)].
After TXA-treatment, hMSCs and chondrocytes that were used for biochemical investigations were trypsinated and seeded in new 96-well-plates (Greiner Bio-One GmbH) at a density of 3 x 10³ cells per cm².
ATP assays were performed after 10 min, 24 h and 48 h. According to the user’s guide, the cells were mixed with 100 μL of CellTiter-Glo^® (Promega GmbH, Mannheim, Germany) reagent, a composition of CellTiter-Glo^® substrate with CellTiter-Glo^® buffer. Cells were incubated in this reagent for 10 min before luminescence was measured using a plate-reading luminometer (Promega GmbH).

Wagenbrenner M., Heinz T., Horas K., Jakuscheit A., Arnholdt J., Mayer-Wagner S., Rudert M., Holzapfel B.M, & Weißenberger M. (2020). Impact of Tranexamic Acid on Chondrocytes and Osteogenically Differentiated Human Mesenchymal Stromal Cells (hMSCs) In Vitro. Journal of Clinical Medicine, 9(12), 3880.

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PgP-Glo Assay for ATPase Activity

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Reagents were prepared according to manufacturer’s protocol. Then, 20 μl of PgP-Glo assay buffer was added to wells labeled “no treatment” (NT). Twenty microliters of 0.25 mM Na₃VO₄ dissolved in PgP-Glo assay buffer was added to the wells labeled Na₃VO₄ on the 96-well plate. Next, 20 μl of 0.5 mM Verapamil in PgP-Glo assay buffer was added to the wells labeled “Ver” and 20 μl of 2.5 X concentrated test compounds was added to the experimental test compound wells. Then, 20 μl of diluted PgP membranes was added to each well and incubated at 37°C for 5 min while floating in a 37°C water bath. Reactions were initiated by adding 10 μl of 25 mM Mg ATP to all wells, and mixing briefly by gentle tapping before placing in the 37°C incubator for 40 min. Fifty microliters of ATP detection reagent was added to each well after removing the plate from the heat source. The plate was mixed briefly and incubated at RT for 20 min to allow luminescent signal to develop. Luminescence was read on a plate-reading luminometer (Promega Corporation, Madison, WI, USA).

Howard C.B., Mcdowell R., Feleke K., Deer E., Stamps S., Thames E., Singh V, & Pervin S. (2016). Chemotherapeutic Vulnerability of Triple-negative Breast Cancer Cell-derived Tumors to Pretreatment with Vernonia amygdalina Aqueous Extracts. Anticancer research, 36(8), 3933-3943.

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