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Specific elisa kit

Manufactured by Thermo Fisher Scientific
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Thermo Fisher Scientific's Specific ELISA kits are laboratory tools designed for the detection and quantification of target analytes in biological samples. These kits employ the enzyme-linked immunosorbent assay (ELISA) technique to provide accurate and sensitive measurements. The core function of these kits is to enable researchers to measure the presence and concentration of specific proteins, hormones, or other molecules of interest in a variety of sample types.

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Lab products found in correlation

Saponin, by Thermo Fisher Scientific (1 mentions) Brefeldin a, by Thermo Fisher Scientific (1 mentions) Brefeldin a, by BioLegend (1 mentions) Pam3csk4, by InvivoGen (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) β mercaptoethanol, by Merck Group (1 mentions) Bca protein assay kit, by GeneCopoeia (1 mentions) Varioskan lux, by Thermo Fisher Scientific (1 mentions) Microplate reader, by Molecular Devices (1 mentions) Triolein, by Merck Group (1 mentions) Egg yolk l α phosphatidylcholine pc lecithin, by Merck Group (1 mentions) Compritol 888, by Gattefosse (1 mentions) Morin hydrate, by Merck Group (1 mentions) Brij52, by BASF (1 mentions) Enspire multimode plate reader, by PerkinElmer (1 mentions) Epoch 2, by Agilent Technologies (1 mentions) Fetal bovine serum (fbs), by Euroclone (1 mentions) Rpmi 1640 medium, by Euroclone (1 mentions) Bradford s method, by Bio-Rad (1 mentions) Complete mini protease inhibitor cocktail, by Roche (1 mentions)

Close spelling variants of this product

Protein specific ELISA kits Mouse-specific ELISA kits Rat-specific ELISA kits Specific quantitative sandwich ELISA kits Murine cytokine-specific ELISA kits ELISA) kits specific for mice Adiponectin human-specific ELISA kits Human-specific ELISA kits Mouse-specific total Tau ELISA kit Cytokine-specific ELISA kits

53 protocols using specific elisa kit

1

Cytokine Profiling in Tissues

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Tissue levels of TNF-α, IL-1β, IL-6, IL-4, IL-13, and IL-10 were measured using specific ELISA kits (all from eBioscience, San Diego, CA, USA).
Koo J.H., Jang H.Y., Lee Y., Moon Y.J., Bae E.J., Yun S.K, & Park B.H. (2019). Myeloid cell-specific sirtuin 6 deficiency delays wound healing in mice by modulating inflammation and macrophage phenotypes. Experimental & Molecular Medicine, 51(4), 48.
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2

Cytokine Profiling of TLR-Stimulated Myeloid Cells

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BMDMs or BMDCs were stimulated with different ligands for TLRs: 100 ng/ml Pam3CSK4 (InvivoGen), 10 µg/ml poly(I:C) RNA (Sigma-Aldrich), 100 ng/ml LPS, 10 µg/ml R848 (InvivoGen), or 5 µM CpG-C DNA (ODN2395; InvivoGen) for 6 h. TNF, IL-12, or IFN-β protein levels in cell culture supernatants were then determined by specific ELISA kits (eBioscience) according to the manufacturer’s protocol. For the detection of intracellular cytokines, cells were stimulated with various TLR ligands in the presence of brefeldin A (eBioscience) for blockade of Golgi function. Cells were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.1% saponin (eBioscience), and subjected to intracellular FACS staining using antibodies against TNF (BioLegend).
After stimulation of differentiated THP-1 cells with TLR ligands in the presence of brefeldin A, cells were surface stained with anti–human CD14 (BioLegend) followed by intracellular staining with anti–human TNF (BioLegend).
Westphal A., Cheng W., Yu J., Grassl G., Krautkrämer M., Holst O., Föger N, & Lee K.H. (2017). Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor–mediated inflammatory responses. The Journal of Experimental Medicine, 214(1), 227-244.
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3

Inflammatory Cytokines Measurement

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Inflammatory cytokines including tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) were measured using specific ELISA kits (eBioscience, USA).
Huang Q.H., Xu L.Q., Liu Y.H., Wu J.Z., Wu X., Lai X.P., Li Y.C., Su Z.R., Chen J.N, & Xie Y.L. (2017). Polydatin Protects Rat Liver against Ethanol-Induced Injury: Involvement of CYP2E1/ROS/Nrf2 and TLR4/NF-κB p65 Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 7953850.
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4

Serum and BALF Antibody Measurement

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The serum and BALF antibodies, including IgE, IgG1 and IgG2a, were determined by using specific ELISA kits according to the manufacturer’s instruction (eBioscience, San Diego, CA, USA). Sera samples were assayed at a dilution of 1:200 for IgE analysis, 1:10000 for IgG1 analysis, and 1:100 for IgG2a analysis, and BALF samples were assayed at a dilution of 1:2 for IgE analysis, 1:50 for IgG1 analysis, and 1:1 for IgG2a analysis. Finally, the absorbance (OD) of the wells at 450 nm was read.
Chiang S.R., Lin C.Y., Chen D.Y., Tsai H.F., Lin X.C., Hsu T.C, & Tzang B.S. (2019). The effects of human parvovirus VP1 unique region in a mouse model of allergic asthma. PLoS ONE, 14(5), e0216799.
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5

Assess OprF-specific Immune Responses

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OprF-specific cellular immune responses were assessed 2 weeks after the immunization with AdC7OprF.RGD or AdC7Null. Mice splenocytes were isolated as a single-cell suspension and cultured in RPMI medium supplemented with 2% fetal bovine serum (HyClone, Logan, UT), 10 mM HEPES (pH 7.5; Biosource International, Camarillo, CA), and 10 μM β-mercaptoethanol (Sigma-Aldrich) in 96-well (Millipore) plates. The splenocytes were stimulated with 10 μg/ml OprF for 48 h and culture supernatants were collected. The mouse Interferon-γ (IFN-γ) and IL-4 were measured in the culture supernatant with specific ELISA kits (eBioscience).
Gomi R., Sharma A., Wu W., Sung B, & Worgall S. (2017). Post-Exposure Immunization by Capsid-Modified AdC7 Vector Expressing Pseudomonas aeruginosa OprF Clears P. aeruginosa Respiratory Infection. Vaccine, 35(51), 7174-7180.
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6

Serum and Nasal Cytokine Profile

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Serum OVA-specific immunoglobulin E (IgE), histamine and leukotrienes C4 (LTC4) levels were assayed using specific ELISA kit (BlueGene Biotech, Shanghai, China) and concentrations of IL-4, IL-9 and IL-17 in NLF or IL-6 and IL-8 in the cell culture supernatants were assayed with specific ELISA kits (eBioscience Inc., USA). Each sample was analyzed in triplicate.
Lin H., Zheng C., Li J., Yang C, & Hu L. (2015). Lentiviral shRNA against KCa3.1 inhibits allergic response in allergic rhinitis and suppresses mast cell activity via PI3K/AKT signaling pathway. Scientific Reports, 5, 13127.
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7

Quantification of Inflammatory Markers

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specific ELISA kits were used to measure the levels of S100A8, S100A9 (MyBioSource,
San Diego, CA, USA) and S100A8/A9 heterodimer (R&D Systems, Minneapolis, MN,
USA). Levels of TNFα and IL-10 were determined using specific ELISA kits
(eBioscience) according to the manufacturer’s instructions. Each sample was
run in duplicate.
Dai J., Kumbhare A., Youssef D., McCall C.E, & El Gazzar M. (2017). Intracellular S100A9 Promotes Myeloid-Derived Suppressor Cells during Late Sepsis. Frontiers in Immunology, 8, 1565.
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8

Cytokine quantitation in BMMC supernatants

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Supernatants for cytokine quantitation were collected 3 or 24 h after BMMCs stimulation. Supernatants were assessed for TNF-α, IL-6, IL-13, and IL-4 using specific ELISA kits (eBiosciences) according to manufacturer's instructions.
De Zuani M., Paolicelli G., Zelante T., Renga G., Romani L., Arzese A., Pucillo C.E, & Frossi B. (2018). Mast Cells Respond to Candida albicans Infections and Modulate Macrophages Phagocytosis of the Fungus. Frontiers in Immunology, 9, 2829.
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9

Cytokine Quantification via ELISA

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Cytokine levels were determined using specific ELISA kits (eBioscience) according to the manufacturer’s instructions. Each sample was run in duplicate.
Dai J., Kumbhare A., Williams D.A., Youssef D., Yao Z.Q., McCall C.E, & Gazzar M.E. (2017). Nfia deletion in myeloid cells blocks expansion of myeloid-derived suppressor cells during sepsis. Innate Immunity, 24(1), 54-65.
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10

Polyphenol-rich extracts modulate pro-inflammatory markers

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Cells were pre-incubated overnight in 24-well plates at a density of 37 × 103 cells/well. The day after, they were treated with H2O2 (200 μM), TNFα (5 ng/mL) or LPS (1 μg/mL) in the presence or not of each polyphenol-rich plant extract (25 μM GAE). After 24 h, cell culture media were collected and stored at −20°C until analysis. Levels of the pro-inflammatory markers IL-6, TNFα and MCP-1 were determined by using specific ELISA kits (eBioscience, UK) and normalized according to total cellular protein amounts determined by Bradford assay [32 (link)].
Marimoutou M., Le Sage F., Smadja J., Lefebvre d’Hellencourt C., Gonthier M.P, & Robert-Da Silva C. (2015). Antioxidant polyphenol-rich extracts from the medicinal plants Antirhea borbonica, Doratoxylon apetalum and Gouania mauritiana protect 3T3-L1 preadipocytes against H2O2, TNFα and LPS inflammatory mediators by regulating the expression of superoxide dismutase and NF-κB genes. Journal of Inflammation (London, England), 12, 10.
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