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Quantichrom creatinine assay kit

Manufactured by BioAssay Systems
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The QuantiChrom Creatinine Assay Kit is a colorimetric assay that measures creatinine levels in biological samples. The kit provides a simple, direct, and accurate method for determining creatinine concentrations.

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Close spelling variants of this product

Creatinine assay kit (44 citations) Quantichrom Creatinine Assay (8 citations) QuantiChrom Urea and Creatinine Assay Kits (5 citations) QuantiChrom Creatinine kit (4 citations) Creatinine kit (3 citations) Creatinine (3 citations) QuantiChrom kits (2 citations)

166 protocols using quantichrom creatinine assay kit

1

Measuring Urinary Albumin-Creatinine Ratio

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We measured the urinary albumin and creatinine levels using the ELISA kit, Albuwell M (Exocell, Philadelphia, United States), and the QuantiChromTM Creatinine Assay Kit (Bioassay systems, CA, United States), respectively, following the manufacturer’s instructions. Urinary albuminuria levels were expressed as albumin/creatinine ratio (uACR, μg/mg).
Song H., Zhang M., Li X., Xu F., Zhang D., Zhu X., Zhang J., Qin W., Shi S, & Wen J. (2021). Generation and Characterization of Mouse Models of C3 Glomerulonephritis With CFI D288G and P467S Mutations. Frontiers in Physiology, 12, 649801.
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2

Quantitative Biomarker Measurement

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The urine albumin, NAG, creatinine and serum creatinine concentrations were measured with an ELISA kit (Exocell; Shibayagi, Gunma, Japan), a NAG test (Nanjing Jiancheng Bioengineering Institute; Nanjing, China) and QuantichromTM creatinine assay kit (Bioassay system; CA), respectively. For all of the assays, the samples were run in duplicate and the results were averaged.
Chen C., Lu C., Qian Y., Li H., Tan Y., Cai L, & Weng H. (2017). Urinary miR-21 as a potential biomarker of hypertensive kidney injury and fibrosis. Scientific Reports, 7, 17737.
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3

Urinary Albumin and Creatinine Measurement

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Before the mice were euthanized, they were provided water ad libitum, and 24-h urine was collected in metabolic cages. The urinary albumin concentration was measured by using a Mouse Albumin ELISA Quantitation Set (Bethyl Laboratories, Inc., Montgomery, TX, USA). The urinary creatinine concentration in the same sample was measured by using the QuantiChromTM Creatinine Assay Kit (BioAssay Systems, Hayward, CA, USA) according to the manufacturer’s protocol.
Zhang L., Zhou F., Yu X., Zhu Y., Zhou Y., Liu J., Liu Y., Ma Q., Zhang Y., Wang W, & Chen N. (2019). C/EBPα deficiency in podocytes aggravates podocyte senescence and kidney injury in aging mice. Cell Death & Disease, 10(10), 684.
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4

Serum Creatinine Quantification

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Creatinine levels were evaluated in 30 μl of serum from each mouse using the QuantiChromTM Creatinine Assay Kit (Bio Assay Systems, Hayward, CA, USA) following the manufacturer's instructions.
Xu S., Lee E., Sun Z., Wang X., Ren T., Zou Z., Jin J., Li J., Zhang J., Li Y., Yang Q., Zhang Y., Guo M., Fang Y, & Ding X. (2021). Perilipin 2 Impacts Acute Kidney Injury via Regulation of PPARα. Journal of Immunology Research, 2021, 9972704.
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5

Kidney and Liver Function Assessment

Cited 1 time
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Kidney and liver functionality were assessed as previously described (n = 6/group), using the following kits: QuantiChromTM Urea Assay Kit, QuantiChromTM Creatinine Assay Kit, EnzyChromTM Aspartate Transaminase Assay Kit and EnzyChromTM Alanine Transaminase Assay Kit (BioAssay Systems)9 (link).
Andreata F., Bonizzi A., Sevieri M., Truffi M., Monieri M., Sitia L., Silva F., Sorrentino L., Allevi R., Zerbi P., Marchini B., Longhi E., Ottria R., Casati S., Vanna R., Morasso C., Bellini M., Prosperi D., Corsi F, & Mazzucchelli S. (2020). Co-administration of H-ferritin-doxorubicin and Trastuzumab in neoadjuvant setting improves efficacy and prevents cardiotoxicity in HER2 + murine breast cancer model. Scientific Reports, 10, 11425.
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6

Quantification of Urinary Biomarkers by ELISA

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All of the utilized enzyme-linked immunosorbent serum assays (ELISAs) are listed in Table 2. The assays were measured according to manufacturer instructions (Nordic Bioscience), and all except sauC3M (sandwich ELISA) were competitive ELISAs. Each ELISA plate included kit control samples to monitor inter-assay variation. All samples were measured within the range of the specific assay. All samples below the lower limit of quantification (LLOQ) were assigned the value of LLOQ. Urinary biomarker levels were normalized for urinary creatinine concentration, measured with the QuantiChromTM Creatinine Assay Kit (Bioassay Systems, Hayward, USA). Samples analyzed for biomarkers in the present study were stored at −80° Celsius.
Rasmussen D.G., Boesby L., Nielsen S.H., Tepel M., Birot S., Karsdal M.A., Kamper A.L, & Genovese F. (2019). Collagen turnover profiles in chronic kidney disease. Scientific Reports, 9, 16062.
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7

Serum Creatinine and Blood Urea Assay

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Serum Creatinine (SCr) and Blood urea nitrogen (BUN) levels were determined using a QuantiChromTM Creatinine Assay Kit and a Bioassay System urea assay kit (BioAssay System, Hayward, CA) according to the manufacturer’s instruction.
Wu Y., Huang L., Sai W., Chen F., Liu Y., Han C., Barker J.M., Zwaini Z.D., Lowe M.P., Brunskill N.J, & Yang B. (2023). HBSP improves kidney ischemia-reperfusion injury and promotes repair in properdin deficient mice via enhancing phagocytosis of tubular epithelial cells. Frontiers in Immunology, 14, 1183768.
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8

Creatinine Colorimetric Assay Protocol

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This colorimetric assay was performed according to the manufacturer’s protocol (QuantiChromTM Creatinine Assay Kit; BioAssay Systems, Hayward, CA).
Alaridhee H., Alharbi A., Saeed Z., Thomas R.C, & Stover C.M. (2020). Complement Properdin Determines Disease Activity in MRL/lpr Mice. Medicina, 56(9), 430.
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9

Quantitative Urea and Creatinine Assay

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BUN was determined using a QuantiChromTM Urea Assay Kit (DIUR-500, Bioassay Systems), and urinary creatinine was determined using a QuantiChromTM Creatinine Assay Kit (DICT-500, Bioassay Systems) according to the manufacturer’s instructions.
Xie W., He Q., Zhang Y., Xu X., Wen P., Cao H., Zhou Y., Luo J., Yang J, & Jiang L. (2023). Pyruvate kinase M2 regulates mitochondrial homeostasis in cisplatin-induced acute kidney injury. Cell Death & Disease, 14(10), 663.
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10

Murine Urinary Albumin and Creatinine Quantification

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The murine specific urinary albumin level was measured using a murine microalbuminuria ELISA kit (albuwell M Test kit, Exocell, Inc. Philadelphia; Cosmo Bio Co., LTD). The urinary creatinine levels were measured using a QuantiChromTM Creatinine Assay Kit (BioAssay System). We used SoftMax pro 6.4 to analyze the urinary albumin and creatinine levels.
Takagaki Y., Shi S., Katoh M., Kitada M., Kanasaki K, & Koya D. (2019). Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice. Scientific Reports, 9, 7519.
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