L tryptophan

Manufactured by Yuanye Bio-Technology

Sourced in China

L-tryptophan is an amino acid that serves as a precursor for the synthesis of serotonin, a neurotransmitter involved in regulating mood, sleep, and other physiological processes. It is an essential nutrient that must be obtained from dietary sources.

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5 protocols using l tryptophan

HPLC-based Metabolite Quantification

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HPLC grade methanol and acetonitrile were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Pure distilled water was purchased from Wahaha (Hangzhou, China). HPLC grade formic acid was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China. 2-Chloro-L-phenylalanine, which was used as the internal standard (IS), was provided by Shanghai Macklin Biochemistry Company, Shanghai, China. Standard 3-indoxyl sulfate was obtained from Beijing LeBo Biotech Co., Ltd., Beijing, China. L-Arginine, glutamic acid, L-valine, linoleic acid, L-phenylalanine, L-tryptophan, and L-tyrosine were purchased from Shanghai Yuanye Biotechnology Co. Ltd., Shanghai, China.

Wen Z., He M., Peng C., Rao Y., Li J., Li Z., Du L., Li Y., Zhou M., Hui O., Feng Y, & Yang S. (2019). Metabolomics and 16S rRNA Gene Sequencing Analyses of Changes in the Intestinal Flora and Biomarkers Induced by Gastrodia-Uncaria Treatment in a Rat Model of Chronic Migraine. Frontiers in Pharmacology, 10, 1425.

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Chinese Herbal Intervention for Colitis

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All the Chinese herbs were provided by Tongde Hospital of Zhejiang Province (Hangzhou, China). All the chemical standards, including l (+)-arginine, proline, adenine, succinic acid, iso leucine, leucine, gallic acid, phenylalanine, protocatechuic acid, l-tryptophan, protocatechuic aldehyde, neo-mangiferin, chlorogenic acid, caffeic acid, amygdalin, mangiferin, rutin, liquiritin, luteoloside, naringin, berberrubine, baicalin, quercetin, epimedin A, epimedin B, epimedin C, icarrin, isorhamnetin, glycyrrhizic acid, limonin, aaikosaponin A, and 3-N-butyl-4,4-dihydrophthalide, were purchased from Shanghai Yuanye Bio-Technology Co. Ltd (Shanghai, China). Azoxymethane (AOM) was obtained from Sigma-Aldrich (St. Louis, MO, United States) and dextran sodium sulfate (DSS) from MP Biomedicals (Santa Ana, CA United States). TUNEL apoptosis assay kits, radioimmunoprecipitation assay (RIPA) buffer, BCA protein assay kits, and anti-MMP9 antibody (AF5234) were purchased from Beyotime (Beijing, China). Mouse IL-6 and IL-17A ELISA kits were from LiankeBio (Hangzhou, China), and antibodies to p-STAT3 (AF3293), STAT3 (AF6294), NF-κB-p65 (BF8005), Bcl-2 (BF9103), Lamin B (AF5161), and β-actin (T0022) were purchased from Affinity Biosciences (Changzhou, China). Antibodies to IL-17RA (sc-376374) and Bax (sc-20067) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States).

Luo Q., Huang S., Zhao L., Liu J., Ma Q., Wang Y., Dong Y., Li C, & Qiu P. (2022). Chang qing formula ameliorates colitis-associated colorectal cancer via suppressing IL-17/NF-κB/STAT3 pathway in mice as revealed by network pharmacology study. Frontiers in Pharmacology, 13, 893231.

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Amino Acid Quantification in Beers

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l-Lysine, l-histidine, l-aspartate, l-cysteine, l-tryptophan, l-serine, l-phenylalanine, l-glycine, l-valine, l-alanine, l-leucine, l-isoleucine, l-threonine, l-methionine, l-proline, and l-glutamic acid were purchased from Yuanye Biological Technology Co., Ltd. (Shanghai, China; purity >99.5%). Copper sulfate and sodium acetate were purchased from Shuang Shuang Chemical Reagent Co., Ltd (Yantai, China). The purified water used to prepare various solutions was obtained from the GLP Laboratory of Lanzhou University (Lanzhou, China). Acetic acid (HPLC grade) was purchased from Shandong Yu Wang Pharmaceutical Co., Ltd. (Shandong, China). Three kinds of beers were purchased from a local supermarket (Lanzhou, China).

Luo T., Ke J., Xie Y, & Dong Y. (2017). Determination of underivatized amino acids to evaluate quality of beer by capillary electrophoresis with online sweeping technique. Journal of Food and Drug Analysis, 25(4), 789-797.

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Lipid Chromatography Protocol

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Methanol and acetonitrile (analytical gradient grade) for lipid chromatography were purchased from Merck (Darmstadt, Germany). Deionized water was produced using a Mill-Q ultrapure water system (Millipore, USA). HPLC grade formic acid was obtained from Tianjin Kermel Chemical Reagent Company (Tianjin, China). 2-Chloro-L-Phenylalanine for use as an internal standard (IS) was provided by Shanghai Macklin Biochemical Company (Shanghai, China). Standard eicosapentaenoic acid, palmitoleic acid, glycocholic acid, gamma-Linolenic acid, arachidonic acid, thymidine, linoleic acid, L-Phenylalanine, L-Tryptophan, and taurocholic acid were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

Zhang X., Li J., Xie B., Wu B., Lei S., Yao Y., He M., Ouyang H., Feng Y., Xu W, & Yang S. (2018). Comparative Metabolomics Analysis of Cervicitis in Human Patients and a Phenol Mucilage-Induced Rat Model Using Liquid Chromatography Tandem Mass Spectrometry. Frontiers in Pharmacology, 9, 282.

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Synthesis of Inorganic Compounds and Pesticides

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Potassium chloride (KCl), silver nitrate (AgNO₃), potassium dihydrogen phosphate (KH₂PO₃) and disodium hydrogen phosphate (NaH₂PO₃) were purchased from Sinopharm Group Chemical Reagent Co., Ltd., Shanghai, China. Copper dichloride dihydrate (CuCl₂ · 2H₂O) and cobalt nitrate (Co(NO₃)₂) were purchased from Tianjin Damao chemical reagent factory, Tianjin, China. Sodium chloride (KCl), cadmium chloride (CdCl₂), chromium trichloride (CrCl₃), calcium sulfate (CaSO₄) and aluminum nitrate (Al(NO₃)₃) were purchased from Xilong Chemical, Shantou, China. Zinc nitrate hexahydrate (Zn(NO₃)₂ · 6H₂O) and magnesiumchloridehexahydrate (MgCl₂) were purchased from Jiuding Chemical Technology Co., Ltd., Shanghai, China. Monocrotophos, chlorpyrifos and methyl parathion were obtained from Beijing Boao Yisi Biotechnology Co., Ltd., Beijing, China. L-tryptophan, propyl bromophosphorus, glufosinate-ammonium, glyphosate, chlorpyrifos methyl and thiophanate methyl were acquired from Yuanye Biotechnology Co., Ltd., Wuhan, China.

Yue X., Zhu C., Gu R., Hu J., Xu Y., Ye S, & Zhu J. (2022). Copper-Modified Double-Emission Carbon Dots for Rapid Detection of Thiophanate Methyl in Food. Foods, 11(21), 3336.

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