For imaging of amyloid-β aggregates, we used APP/PS1 (The Jackson Laboratory, B6.Cg-Tg (APPswe, PSEN1-dE9)85Dbo/Mmjax) double transgenic mice expressing mutant human APPswe/human PS1-ΔE944 (link) along with age-matched non-transgenic littermates. Mice of both sexes were used at 8–9 months of age for through-skull imaging measurements and 10–12 months of age for in vivo characterization of candidate NIR fluorophores. For comparing different age groups, the young and old cohorts consisted of mice at 7–8 months and 22–24 months of age, respectively. For imaging neurofibrillary tangles, we used rTg4510 (The Jackson Laboratory, Tg (Camk2a-tTA)1Mmay Fgf14Tg (tetO-MAPT*P301L)4510Kh) mice expressing the tetracycline-controlled transactivator protein (tTA)45 (link). Mice of both sexes at 6–8 months of age were used. C57BL/6J (The Jackson Laboratory) male mice at 3 months of age were used for blood pharmacokinetic studies, brain clearance and FLIM measurements. CD-1 (Charles River Laboratories) male mice at 6–8 weeks were used for biodistribution studies. Animals were housed in an AAALAC-certified facility (Massachusetts General Hospital, D16–00361), and all animal studies were performed under the supervision of the Massachusetts General Hospital Institutional Animal Care and Use Committee in accordance with the approved institutional protocol (2018N000131).
App ps1
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
The APP/PS1 is a transgenic mouse model that expresses both the human amyloid precursor protein (APP) and presenilin 1 (PS1) genes. These mice develop age-dependent amyloid-beta (Aβ) deposition and other pathological features characteristic of Alzheimer's disease. The model is commonly used in Alzheimer's disease research to study the progression of the disease and evaluate potential therapeutic interventions.
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Lab products found in correlation
C57bl 6j, by Jackson ImmunoResearch (4 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (3 mentions)
App ps1 mice, by Jackson ImmunoResearch (2 mentions)
C57bl 6, by Jackson ImmunoResearch (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Appswe psen1de9, by Jackson ImmunoResearch (1 mentions)
Sprague dawley, by Charles River Laboratories (1 mentions)
Microtome, by Leica (1 mentions)
Triton x, by Merck Group (1 mentions)
Normal goat serum (ngs), by Merck Group (1 mentions)
Normal goat serum (ngs), by Thermo Fisher Scientific (1 mentions)
Male c57bl 6, by Jackson ImmunoResearch (1 mentions)
Fisher superfrost plus slides, by Thermo Fisher Scientific (1 mentions)
Td 08811, by Inotiv (1 mentions)
Td2018, by Inotiv (1 mentions)
Microtainer tube, by BD (1 mentions)
5 bromo 2 deoxyuridine brdu, by Merck Group (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
28 protocols using app ps1
1
Imaging of Amyloid-β and Neurofibrillary Tangles
2
Transgenic Mouse Model of Alzheimer's
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APP/PS1 or B6C3-Tg(/APPswe,PSEN1dE9/)85Dbo/J mice were obtained from the Jackson Laboratory and maintained in the institute's animal house facility. These transgenic mouse lines for AD express human APPswe mutations (K670N and M671L) and exon-9-deleted human presenilin 1(PSEN1dE9) under the control of the mouse prion gene promoter. Animals were provided water and food ad libitum. The genotyping was performed using PCR as described previously (Ghate et al., 2014 (link)).
AD mice, along with controls at their ages of 8 and 12 months, were anaesthetized with xylazine (10 mg/kg body weight) and ketamine (100 mg/kg body weight) and perfused transcardially with PBS followed by 4% paraformaldehyde (w/v) in PBS. Brains were collected and further placed in 4% paraformaldehyde for 24 h and then treated with 10, 20 and 30% sucrose (in PBS) followed by sectioning in a freezing microtome (20 μm thickness). Sections (both control and AD) were placed on the same slides.
AD mice, along with controls at their ages of 8 and 12 months, were anaesthetized with xylazine (10 mg/kg body weight) and ketamine (100 mg/kg body weight) and perfused transcardially with PBS followed by 4% paraformaldehyde (w/v) in PBS. Brains were collected and further placed in 4% paraformaldehyde for 24 h and then treated with 10, 20 and 30% sucrose (in PBS) followed by sectioning in a freezing microtome (20 μm thickness). Sections (both control and AD) were placed on the same slides.
3
Transgenic APP/PS1 Mouse Model
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The transgenic mice (APP/PS1) used in this study were obtained from the Jackson Laboratory (Bar Harbor, ME, USA). See the Supporting Information for details.
4
Transgenic Mouse Model for Alzheimer's
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Female APPswe/PSEN1dE9 (henceforward APP/PS1) transgenic mice with 5–9 months were initially purchased from The Jackson Laboratory (MMRRC stock #34832; donated by Dr. David R Borchelt, University of Florida). To maintain the colonies, male hemizygous APP/PS1 mice were crossed with female WT mice. In this study, 8–9 months old mice were obtained by crossing female WT (B6C3F1/J) mice with male hemizygous APP/PS1 mice. All mice were kept in cages with 1–3 littermates and with ad libitum food and water. The cages were kept at 21–23 °C with 50–60% humidity under a 12 h dark/light cycle with light from 7AM to 7PM. All procedures were carried out in accordance with the guidelines of the European Community guidelines (EU Directive 2010/63/EU) and the Portuguese law on animal care (1005/92) and approved by the Ethical Committee of the Center for Neuroscience and Cell Biology of (ORBEA n° 243_2020/1210-72020).
5
Transgenic Mouse Model for Alzheimer's
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APPswe/PS1ΔE9 mice on a B6C3 [B6;C3-Tg(APPswe, PSEN1dE9)85Dbo/mmjax] background (APP/PS1; from Jackson Labs and bred in-house; ref. 36 (link)) were crossed to Kir6.2–/– (C57BL6/J) (33 (link)) or Kir6.1–/– (C57BL6/J) (81 (link)) (both gifts from Washington University School of Medicine in St. Louis and bred in-house) for these studies. To generate APP/PS1 mice with homozygous knockout (–/–) for Kir6.2, we bred Kir6.2–/– mice with APP/PS1 mice, generating Kir6.2+/+ APP/PS1 and Kir6.2–/– APP/PS1 mice for the acute and chronic experiments. For reverse microdialysis experiments, we also generated mice homozygous knockout (–/–) for Kir6.1 crossed to APP/PS1 mice, generating Kir6.1–/– APP/PS1 mice. Mice were given food and water ad libitum and maintained on a 12-hour light/12-hour dark cycle. All procedures were carried out in accordance with an approved IACUC protocol from Washington University School of Medicine or Wake Forest School of Medicine.
6
Cell Transplantation in AD Mouse Models
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The animal experiments were carried out following protocols approved by the Animal Ethics Committee of the Shanghai Institutes for Biological Sciences. Two strains of AD model mice, 5XFAD (Jackson No. 006554) and APP/PS1 (Jackson No. 004462), were purchased from Jackson Laboratory. Subsequent steps in the cell transplantation procedure are detailed in the Supplemental Experimental Procedures .
7
Alzheimer's Disease Mouse Model Protocol
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Protocols for animal use were approved by the Institutional Animal Care and Use Committee at Southern Illinois University School of Medicine (IRB #2022–055) and in accordance with the ARRIVE guidelines. Male and female APP/PS1 and littermate control C57BL/6 mice used for this study were bred and maintained in our animal colony and originated from founder C57BL/6J (RRID:IMSR_JAX:000664) and APP/PS1 (RRID:MMRRC_034832-JAX) mice from Jackson Laboratory (Bar Harbor, ME). A 5 mm tail tip was sent to TransnetYX®, Inc (Cordova, TN) to confirm genotypes. Mice were group-housed according to sex and genotype on a 12:12 hour light / dark cycle, and laboratory rodent diet (LabDiet, 5001) and water were available ad libitum. One week post cognitive assessment, mice were deeply anesthetized with isoflurane and a cardiac puncture for blood chemistry analysis was performed. Immediately following, mice were euthanized by decapitation. Tissues were extracted and stored at −80°C until processing.
8
Transgenic Mouse Model of Alzheimer's
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A 20-week study design with two different diet compositions was used in this study. Transgenic APP/PS1 mice expressing APP and mutant PS1 from Jackson Laboratories [22 (link)] were bred with transgenic C57BL/6J mice expressing Ad36E4orf1 protein in the adipose tissue upon doxycycline induction [17 (link)]. Details for design and development of the Ad36E4orf1 transgenic mice has been previously described [17 (link)]. Genotyping details in supplemental files.
Mice expressing both APP/PS1 and E4orf1 transgene, APP/PS1/E4orf1 were used in the treatment groups and APP/PS1 transgene expressing mice were used as control. Transgenic expression of E4orf1 protein specific to the adipose tissue was confirmed in protein lysates from ingunal (subcutaneous) adipose depots, immunoblotted with E4orf1 antibody (Fig.S1 ). Mice were obtained from different breeding pairs. During experiments the mice were assigned numbers and the experimenter was blinded to their group assignment.
Mice expressing both APP/PS1 and E4orf1 transgene, APP/PS1/E4orf1 were used in the treatment groups and APP/PS1 transgene expressing mice were used as control. Transgenic expression of E4orf1 protein specific to the adipose tissue was confirmed in protein lysates from ingunal (subcutaneous) adipose depots, immunoblotted with E4orf1 antibody (Fig.
9
APP/PS1 Mouse Model for Alzheimer's
10
Transgenic Alzheimer's Mouse Models
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