To bioprint BoneMA in various geometrical shapes, specific print patterns were designed using CAD (Fusion 360, AutoDesk) and converted into image slices with the accompanying 3D printing software (Print Studio, Autodesk). The print patterns were designed arbitrarily with star, square, triangle, and rhombus shapes, as well as flower, spiral, concentric circles, and the OHSU logo. Shapes were printed with length and width dimensions that ranged from 600 μm (square) to 2.5 mm (OHSU logo) with a thickness of 450 μm, under printing exposures of 25 seconds. For bioprinting of the said geometries, the bioink was formulated by mixing HMSCs with the BoneMA (5% (w/v)) pre-polymer containing LAP (0.15% (w/v)) at a concentration of 5 × 105 cells/ml. The HMSC-laden bioprinted BoneMA was fixed with 4% (v/v) paraformaldehyde and permeabilized using 0.1% (v/v) Triton X-100. Next, the samples were treated with 1.5% (w/v) bovine serum albumin (Sigma Aldrich) in DPBS for 1h, followed by Image-iT FX signal enhancer (Invitrogen, CA) for 30 min, after which they were immunostained with ActinGreen™ 488 ReadyProbes™ (Invitrogen).
Print studio
Manufactured by Autodesk
Print Studio is a 3D printing software application developed by Autodesk. It provides users with tools to prepare, preview, and manage 3D print jobs. The software supports a variety of 3D printing technologies and file formats.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using print studio
1
Bioprinting BoneMA Geometrical Shapes
2
Resin-Based Microfluidic Device Fabrication
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All DLP files were created using CAD software SolidWorks, imported and sliced to photopatterns in Autodesk Print Studio, and converted to tar.gz format before being sent to print on Autodesk Ember. The aluminum substrate on the printhead was anodized to black to suppress surface reflection and enhance adhesion between the printed part and the printhead (Surface Finish Technologies Inc.). A 3-mm-thick PMP window (RT18, Goodfellow Corp.) was attached to a custom-built polytetrafluoroethylene resin tray and bonded using a silicone adhesive (Sil-Poxy, Smooth-On Inc.) after 45 s of oxygen plasma activation. The oligomerized liquid resin was transferred to the resin tray and let stand still for 5 min before printing. The xy resolution of the printer was measured as 50 μm (1280 × 800 pixels of UV projector over the maximum build area of 64 mm by 40 mm) for thinner structures (h < 200 μm, <10 layers) and ~100 to 200 μm for thicker structures (200 μm < h < 800 μm, 10 to 40 layers) because of stronger light scattering. The z resolution was 10 μm. Postcuring under higher-intensity UV (25 mW cm−2) was performed on all printed parts both sides for 90 s, followed by 70°C vacuum bakeout for 24 hours to remove all residue solvents.
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