Thin sections of lung tissue were subjected to deparaffinization, rehydration and antigen recovery. Endogenous peroxidases were then blocked, followed by tissue permeabilization using 0.4% Triton X-100 in PBS. Nonspecific binding was blocked, and the thin sections were washed twice with PBS containing 0.05% Tween 20 and incubated with anti-SARS-CoV-2 spike glycoprotein rabbit antibody (Abcam, ab272504, 1:100) for one hour at room temperature. The thin sections were then washed with PBS containing 0.05% Tween 20 twice and incubated with the Alexa Fluor 647-conjugated anti-rabbit antibody (Thermo Fisher Scientific; A21244, 1:100) for 30 min in a dark chamber at room temperature. After washing, anti-horse IgG FITC (Sigma-Aldrich; F-7759, 1:100) was added and incubated for one hour at room temperature. The thin sections were washed, and the slides were mounted using Hoechst (Thermo Fisher Scientific; 62249, 1:1000) and ProLong™ Glass Antifade Mountant (Thermo Fisher Scientific; P36980). The sections were analysed under a confocal microscope (Leica TSC SP8 DSL Hyvolution). The images were assembled and analysed in 3D using Imaris Viewer software.
Anti horse igg fitc
Manufactured by Merck Group
Anti-horse IgG FITC is a fluorescently labeled antibody that binds specifically to horse immunoglobulin G (IgG). It is commonly used in research applications that require the detection or quantification of horse IgG in various sample types.
Automatically generated - may contain errors
2 protocols using anti horse igg fitc
1
SARS-CoV-2 Spike Glycoprotein Immunofluorescence
2
Indirect Immunofluorescent Assay for Anti-Toxoplasma IgG
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Analyses to detect anti-T. gondii IgG antibodies were performed at the Laboratory for Toxoplasmosis and Other Protozoan Diseases (LabTOXO) of the Oswaldo Cruz Institute (IOC), Fiocruz. Samples were analyzed by means of the indirect immunofluorescent antibody test (IFAT), which was performed as described by Camargo (1964) and in accordance with the protocol established at LabTOXO. Tachyzoites of the RH strain of T. gondii that were maintained in Swiss Webster mice were used as the antigen. Positive and negative controls that had previously been tested, and which were stored at the serum bank of LabTOXO, were also used. The commercial conjugate anti-horse IgG FITC (Sigma-Aldrich ) was used in all reactions, at a dilution of 1:75 in Evan's Blue. Samples were considered positive when formation of the antigen-antibody-fluorescein conjugate complex was observed at a dilution of 1:64 or greater, thus showing total fluorescence of the tachyzoite surface.
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