Abi q6 sequence detection system machine

Peripheral blood was collected from KD patients after treatment completion. Genomic DNA was extracted with a TIANamp Blood DNA Kit (DP318, TIANGEN Biotech, Beijing) following the guidance of the manufacturer’s instructions (Wu et al., 2020 (link)). Specific fluorescent allele probes for rs4594236 were purchased from ABI (Thermo Fisher Scientific, United States). PCR was performed in 384-well plates with an ABI-Q6 Sequence Detection System machine (Thermo Fisher Scientific).
The genotyping of the SNP was conducted using a TaqMan SNP genotyping assay (Lin et al., 2021 (link)). Laboratory technicians were blind to the sample information, including the identities of the replicate aliquots. 10% of the samples from both groups were arbitrarily chosen to repeat the genotyping results. A concordance rate of 100% was obtained.

Peripheral blood was collected from patients with KD. Genomic DNA was extracted with the TIANamp Blood DNA Kit (DP318, TIANGEN Biotech, Beijing) followed by the guidance of the manufacturer's instructions. Specific fluorescent allele probes for rs9965664 were purchased from ABI (Thermo Fisher Scientific, United States). PCR was performed in 384-well plates with an ABI-Q6 Sequence Detection System machine (Thermo Fisher Scientific).
The genotyping of the SNP was conducted using the TaqMan SNP genotyping assay. Laboratory technicians were blind to the sample information, including the identities of the replicate aliquots. About 10% of the samples from both groups were arbitrarily chosen to repeat the genotyping results. A concordance rate of 100% was obtained.

Genomic DNA was extracted from anticoagulant-containing peripheral blood collected from patients using the TIANamp Blood DNA Kit (DP318, TIANGEN Biotech, Beijing) according to the manufacturer's instructions. The procedures can be found in our previous paper (12 (link)). The PLA2G7 rs1051931 G>A polymorphism was genotyped with TaqMan method. Allele-specific probes were ordered from Applied Biosystems. PCR was performed in 384-well plates with an ABI-Q6 Sequence Detection System machine (Thermo Fisher Scientific). Additionally, in order to ensure the quality and accuracy of the genotyping results, we randomly selected 10% of the samples for repeated analysis, and the results were completely consistent.