Minimum essential medium (mem)

HCC cell lines were obtained from iCell Bioscience (China). The cells were routinely cultured in MEM (iCell Bioscience, Shanghai, China) for HepG2 and SK-Hep1 cells or in DMEM (Biological Industries, Beit-Haemek, Israel) containing nonessential amino acids (Invitrogen, Waltham, MA, USA) for Huh7 cells, and the media were supplemented with 10% fetal bovine serum (Biological Industries, Beit-Haemek, Israel). The cells were cultured at 37 °C with 5% CO₂ humidified air according to standard procedures.
Transient plasmid transfection was performed with XtremeGENE HP DNA Transfection Reagent (Roche, Switzerland) following the manufacturer’s instructions.

The human bladder cancer cell line J82 cells were cultured in MEM Alpha Medium (Gibco, USA). The RT4 cells and T24 cells were maintained in McCoy’s 5A Medium (iCell, China), and the 5637 cells were cultured in RPMI1640 Medium (iCell, China). The UMUC3 cells and TCCSUP cells were cultured in MEM (iCell, China), and the SW780 cells were maintained in L15 Medium (iCell, China). The human urothelial cells line SV-HUC-1 cells were cultured in F12K medium (iCell, China). All of the mediums were supplemented with 10% fetal bovine serum (FBS, BI, ISR), 100 μg/mL of penicillin, and 100 U/mL of streptomycin (Invitrogen). All of the examined cells were grown at 37°C with 5% CO₂.
The SCD inhibitor (A939572) utilized in this study was purchased from MedChem Express (HY-50709).

HEK-293T (Procell CL-0005), A-498 (Procell CL-0254) and 786-O (Procell CL-0010) cell lines were kindly provided by Procell Life Science and Technology Co., Ltd. (Wuhan, China), and were correctly identified by STR. RPTEC (FY-22FN1580) cells were kindly provided by Fuyu Biotechnology Co., Ltd. (Wuhan, China), and were correctly identified by STR. HEK-293T and RPTEC cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Procell); A-498 and 786-O cells were cultured in Minimum Essential Medium (MEM, iCell) and Roswell Park MEMorial Institute (RPMI-1640, Biosharp) separately with 10% fetal bovine serum (FBS, Procell) and 1% penicillin and streptomycin (100 U/mL penicillin and 100 µg/mL streptomycin, Biosharp) in a 37 °C incubator containing 5% CO₂.