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Atp tlrl atpl

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ATP (tlrl-atpl) is a laboratory product provided by InvivoGen. It is the molecular formula for adenosine triphosphate, a key energy-carrying compound found in the cells of all living organisms.

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Lab products found in correlation

Lps l2880, by Merck Group (2 mentions) Goat anti mouse hrp, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit hrp, by Santa Cruz Biotechnology (1 mentions) Cocaine hydrochloride c5776, by Merck Group (1 mentions) Caspase 1 p20, by Santa Cruz Biotechnology (1 mentions) Cd11b, by Novus Biologicals (1 mentions) Il 1 beta, by Abcam (1 mentions) Mcc950 cp 456773, by Merck Group (1 mentions) Strataclean resin, by Agilent Technologies (1 mentions) Aicar s1802, by Selleck Chemicals (1 mentions) Compound c p5499, by Merck Group (1 mentions) Anti ha h6908, by Merck Group (1 mentions) Pt172 ampk, by Cell Signaling Technology (1 mentions) Anti gsdmd ab219800, by Abcam (1 mentions) Anti il18 a1115, by ABclonal (1 mentions) Cd8 percp cy5.5 clone 53 6 7, by BioLegend (1 mentions) Cd4 apc clone rm4 5, by BioLegend (1 mentions) Torin1, by Bio-Techne (1 mentions) Lps e coli o111 b4, by Merck Group (1 mentions) Rabbit anti akt, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Tlrl-atp (5 citations) Tlrl-atpl (4 citations)

5 protocols using atp tlrl atpl

1

NLRP3 Inflammasome Activation Assay

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Antibodies and reagents used in this work were purchased from the indicated sources: NLRP3 (AdipoGen; AG-20B-0014); ASC (Novus; NB1–78978 for western blots and Adipogen; AG-25B-0006 for IHC), caspase-1 p20 (Santa Cruz; sc-398715), Iba-1 (Novus NB1001028 or Wako; 19–19741), CD11b (Novus; NB11089474), goat anti-mouse-HRP (Santa Cruz Biotechnology; sc-2005) and goat anti-rabbit-HRP (Santa Cruz Biotechnology; sc-2004); IL-1 beta (Abcam; ab9722); actin (Sigma-Aldrich; A1978). Cryopyrin/NLRP3 siRNA (sc-45470) was from Santa Cruz Biotechnology. MCC950/CP-456773 (Sigma; pz0280) was from Sigma-Aldrich. Cocaine hydrochloride (C5776) and LPS (L2880) was from Sigma-Aldrich. Sterile water was used to dissolve cocaine and LPS. ATP (tlrl-atpl) was from Invivogen. FAM-FLICA caspase assay kit (#97) was from ImmunoChemistry
Chivero E.T., Thangaraj A., Tripathi A., Periyasamy P., Guo M.L, & Buch S. (2021). NLRP3 inflammasome blockade reduces cocaine-induced microglial activation and neuroinflammation. Molecular neurobiology, 58(5), 2215-2230.
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2

AMPK Signaling Pathway Modulation

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LPS O111:B4 (L2630), Doxycycline (D3447), 2-Deoxy-D-glucose (D8375) and compound C (P5499) were obtained from Sigma-Aldrich. Subcellular protein fractionation kit (78840) was obtained from Thermo Fisher. Metformin (S1950) and AICAR (S1802) were obtained from Selleck Chemicals. ATP (tlrl-atpl) was obtained from InvivoGen. StrataClean resin (400714) and chemical competent cells (200315) were obtained from Agilent.
For immunoblot, AMPKα1(#2795), AMPKα2 (#2757), AMPKα1/2 (#5831) and pT172-AMPK (#2535) were purchased from Cell Signaling Technology. Anti-Flag (F1804), anti-Flag (F7425) and anti-HA (H6908) were obtained from Sigma-Aldrich. Anti-GSDMD (ab219800) and anti-Caspase-1 (ab108362) were obtained from Abcam. Anti-β-Actin (sc-47778), anti-GST (sc-138) and anti-Na/K-ATPase α1 (sc-21712) were purchased from Santa Cruz Biotechnology. Anti-IL18 (A1115) was obtained from Abclonal Technology. phospho-GSDMD S46 was generated by Abclonal Technology.
For FCAS analysis, CD45-APC-Cy7(clone 30-F11), CD3-FITC (clone 145-2C11), CD8-PerCP-Cy5.5 (clone 53-6.7) and CD4-APC (clone RM4-5) were obtained from BioLegend.
Chu X., Xiao X., Wang G., Uosef A., Lou X., Arnold P., Wang Y., Kong G., Wen M., Minze L.J, & Li X.C. (2023). Gasdermin D-mediated pyroptosis is regulated by AMPK-mediated phosphorylation in tumor cells. Cell Death & Disease, 14(7), 469.
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3

Immunoblotting Techniques for Cellular Signaling

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All media (RPMI, DMEM) and PBS buffers were purchased from Gibco (61870–010; 41966–029; 14190–094). NaCl was purchased from Merck. LPS (E. coli O111:B4) and DPI were purchased from Sigma Aldrich (D2926). Torin1 was purchased from Tocris (4247); E-64d was obtained from Hycultec (HY-15282). Ionomycin (407952) was purchased from CalbioChem. ATP (tlrl-atpl) and bafilomycin A1 (tlrl-baf1) were obtained from Invivogen. The prolyl-hydroxylase inhibitor DMOG was purchased from Cayman Chemical (71210). For immunoblotting, the following antibodies were used: rabbit anti-ACT (A2066; Sigma-Aldrich); rabbit-anti-HSP90A/B (Santa Cruz Biotechnology, sc-7947), rabbit anti-NFAT5 (Thermo Scientific, PA1-023), rabbit anti-MAP1LC3B (Novus Biologicals, NB100-2220), rabbit anti-ATG7 (Cell Signaling Technology, D12B11) rabbit anti-p-AKT (Cell Signaling Technology, 4060S), rabbit anti-AKT (Cell Signaling Technology, 9272S), rabbit anti-SQSTM1 (Sigma Aldrich, P0067), rabbit anti-HIF1A (Cayman Chemical, 10006421). As secondary antibody, we used swine anti-rabbit HRP (P0399, Dako).
Neubert P., Weichselbaum A., Reitinger C., Schatz V., Schröder A., Ferdinand J.R., Simon M., Bär A.L., Brochhausen C., Gerlach R.G., Tomiuk S., Hammer K., Wagner S., van Zandbergen G., Binger K.J., Müller D.N., Kitada K., Clatworthy M.R., Kurts C., Titze J., Abdullah Z, & Jantsch J. (2019). HIF1A and NFAT5 coordinate Na+-boosted antibacterial defense via enhanced autophagy and autolysosomal targeting. Autophagy, 15(11), 1899-1916.
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4

Macrophage Activation and Characterization

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RPMI-1640 cell culture media (#R0833), 200 mM L-glutamine (#G7513), 1% penicillin/streptomycin (#P4333), Accutase (#A6964), mitomycin C (#10107409001), antimycin A from Streptomyces sp. (#A8674), gentamicin (#G1397), dihydroethidium (DHE) (#37291), and gelatin type A (#G1890) were from Sigma-Aldrich. FBS (#P040-37500) and Panexin BMM Serum Substitute (#P04-951SA2) were obtained from PAN Biotech. Ultrapure lipopolysaccharide (LPS) from E. coli K12 (#tlrl-peklps), and ATP (#tlrl-atpl) were purchased from Invivogen. TE buffer (#A0386.1000), and DMSO (#A3672-0250) were from AppliChem, M-CSF (#130-101-705) from Miltenyi Biotech, and mouse recombinant IFN-g (#87389.100) from Biomol.
Primers for qRT-PCR were purchased from Eurofins Genomics. Other chemicals were obtained from either Sigma-Aldrich or Carl Roth unless stated otherwise.
Legroux T.M., Schymik H.S., Gasparoni G., Mohammadi S., Walter J., Libert C., Diesel B., Hoppstädter J, & Kiemer A.K. (2024). Immunomodulation by glucocorticoid-induced leucine zipper in macrophages: enhanced phagocytosis, protection from pyroptosis, and altered mitochondrial function. Frontiers in immunology, 15.
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5

Inflammatory Cytokine Assay Protocol

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Dulbecco’s modified Eagle medium (DMEM, high glucose, 11965092), fetal bovine serum (FBS, 10099141), Lipofectamine™ 2000 (11668019), Pierce™ BCA Protein Assay Kit (23225), and Thermo Scientific RevertAid Mix (M16325) were purchased from ThermoFisher Scientific (Waltham, MA, USA). Protease inhibitor cOmplete™ Cocktail (4693116001) was purchased from Roche (Basel, Switzerland). PerfectStart™ Green qPCR SuperMix (AQ601) was purchased from TransGen Biotech (Beijing, China). NucleoZOL (740404) was purchased from MACHEREY-NAGEL (MN, Düren, Deutschland). Mouse IL-1β ELISA kit (1210122), human IL-1β ELISA kit (1110122), mouse IL-6 ELISA kit (1210602), and mouse TNF-α ELISA kit (1217202) were purchased from DAKEWE (Shenzhen, China). LPS (L2880) was purchased from Sigma (Missouri, USA). ATP (tlrl-atpl) and MSU (tlrl-msu) were purchased from In vivoGen.
Li J., Lin H., Fan T., Huang L., Zhang X., Tai Y., Fang Y., Li Q., Zhao R., Wang P., Zhou L., Wan L., Wu Y., Zhong H., Wei C, & Yang X. (2023). BPOZ-2 is a negative regulator of the NLPR3 inflammasome contributing to SARS-CoV-2-induced hyperinflammation. Frontiers in Cellular and Infection Microbiology, 13, 1134511.
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