Pstat1 tyr701 9167

Manufactured by Cell Signaling Technology

PSTAT1 Tyr701 #9167 is an antibody that recognizes phosphorylated STAT1 at tyrosine 701. It is designed for use in Western blotting applications to detect the phosphorylation state of STAT1.

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Lab products found in correlation

P akt ser473 4060, by Cell Signaling Technology (2 mentions) Akt1 2967, by Cell Signaling Technology (2 mentions) Alexa conjugated antibody, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole dapi sc 3598, by Santa Cruz Biotechnology (2 mentions) Recombinant mouse ifn γ, by Thermo Fisher Scientific (2 mentions) Biotin, by Thermo Fisher Scientific (1 mentions) P gsk 3β 5558, by Cell Signaling Technology (1 mentions) F4 80 monoclonal antibody bm8, by Thermo Fisher Scientific (1 mentions) Mouse serum albumin, by Merck Group (1 mentions) Lithium chloride (licl), by Merck Group (1 mentions) Doxorubicin, by Pfizer (1 mentions) Lipopolysaccharide from escherichia coli 0111 b4, by Merck Group (1 mentions) Azd8055, by AstraZeneca (1 mentions) β actin 4967, by Cell Signaling Technology (1 mentions)

3 protocols using pstat1 tyr701 9167

Comprehensive Immunoblotting Protocol

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2Antibodies against GAPDH sc-322 (Santa Cruz Biotechnology, Santa Cruz, CA), pSTAT1 Tyr701 #9167, pS6 #15967, pAkt Ser473 #4060, Akt1 #2967, pGSK3β #5558, pH2AX (γ-H2AX) mAb #9718, pp53 #9286, CHK1 # 2348 and pBRCA1 #9009 were obtained from Cell Signaling Technology (Danvers, MA). F4/80 Monoclonal Antibody (BM8) coupled to Biotin (eBioscience, Waltham, MA) was used according to manufacturer's instructions. p-Histone H3, ab32107 was obtained from Abcam (Cambridge, MA). Alexa conjugated antibodies were obtained from Thermo Fisher Scientific (Waltham, MA). AffiniPure goat and rabbit anti-horseradish peroxidase (HRP) were obtained from Jackson Immunoresearch (San Diego, CA) and 4′,6-Diamidino-2-phenylindole (DAPI) SC-3598 from Santa Cruz Biotechnology (Santa Cruz, CA). Recombinant mouse IFNγ (PeproTech, Rocky Hill, NJ) was dissolved in 0.002% mouse serum albumin (MSA; Sigma-Aldrich, St. Louis, MO) and used at 2.5 μg/kg of weight. LiCl (Sigma-Aldrich, St Louis, MO) was used at 4 mg/mice and 4 μM in vitro. Rapamycin used as previously reported by us [14] (link) Doxorubicin was obtained from Pfizer and used at 3.45 μM. AZD8055 and XAV939 were used as previously reported by us [16] (link), [17] . Dextran sulfate sodium YD318041799 (Carbosynth, San Diego, CA) was dissolved at 3% in drinking water.

Gutiérrez-Martínez I.Z., Rubio J.F., Piedra-Quintero Z.L., Lopez-Mendez O., Serrano C., Reyes-Maldonado E., Salinas-Lara C., Betanzos A., Shibayama M., Silva-Olivares A., Candelario-Martinez A., Meraz-Ríos M.A., Schnoor M., Villegas-Sepúlveda N, & Nava P. (2018). mTORC1 Prevents Epithelial Damage During Inflammation and Inhibits Colitis-Associated Colorectal Cancer Development. Translational Oncology, 12(1), 24-35.

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Molecular Signaling Pathway Analysis

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Primary antibodies were as follows: Myo1F HPA055242 (Sigma-Aldrich), GAPDH/sc-322 (Santa Cruz Biotechnology®), TNF-α #11948, pSTAT1-Tyr701 #9167, pS6 #15967, pAkt-Ser473 #4060, pAkt-Ser308 #4056, Akt1 #2967 from Cell Signaling Technology®, CD86-PE-CY5 #15-0862-82 (eBioscience), and CD80-PE #553769 (BD pharmingen). Alexa conjugated antibodies and affiniPure goat and rabbit anti-horseradish peroxidase (HRP) were obtained from Thermo Fisher Scientific and Jackson Immunoresearch, respectively. 4′,6-Diamidino-2-phenylindole (DAPI) sc-3598 from Santa Cruz Biotechnology. Recombinant mouse IFN-γ from PeproTech was dissolved in 0.002% mouse serum albumin (MSA; Sigma-Aldrich) and used at 2.5 μg/kg of mice weight. Lipopolysaccharide from Escherichia coli 0111:B4 (Sigma-Aldrich) was dissolved in 0.002% mouse serum albumin and used at 100 μg/mice. AZD8055 [(5-{2,4-Bis[(3S)-3-methylmorpholin-4-yl]pyrido[2,3-d]pyrimidin-7-yl}-2-methoxyphenyl)methanol] from Astra Zeneca was dissolved in DMSO and use at 20 nM. Dextran sulfate sodium (DSS) YD318041799 from Carbosynth was dissolved at 2.5% in tap water.

Piedra-Quintero Z.L., Serrano C., Villegas-Sepúlveda N., Maravillas-Montero J.L., Romero-Ramírez S., Shibayama M., Medina-Contreras O., Nava P, & Santos-Argumedo L. (2019). Myosin 1F Regulates M1-Polarization by Stimulating Intercellular Adhesion in Macrophages. Frontiers in Immunology, 9, 3118.

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Western Blot Analysis of Signaling Proteins

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Western blot was performed as described previously [11 (link)] and antibodies for β-actin (4967), Bcl-xL (2764), Mcl-1 (5453), Stat1 (9172), p-Stat1 (Tyr701) (9167), Stat3 (4904), p-Stat3 (Tyr705) (9138), p-p53 (9281) and p53 (2524) as well as horseradish peroxidase (HRP)-linked anti-mouse (7076) and -rabbit (7074) IgG, purchased from Cell Signaling Technology (Bioké, Leiden, The Netherlands) were used.

Wang J., De Veirman K., De Beule N., Maes K., De Bruyne E., Van Valckenborgh E., Vanderkerken K, & Menu E. (2015). The bone marrow microenvironment enhances multiple myeloma progression by exosome-mediated activation of myeloid-derived suppressor cells. Oncotarget, 6(41), 43992-44004.

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