Ggplot2 2

Mice were dark adapted for at least 12 h before recordings and anesthetized by subcutaneous injection of ketamine (65 mg/kg) and xylazine (13 mg/kg). The pupils were dilated with tropicamide eyedrops (Mydriaticum Stulln; Pharma Stulln). Silver needle electrodes served as a reference (forehead) and ground (tail) and gold wire ring electrodes as active electrodes. Corneregel (Bausch & Lomb, Berlin, Germany) was applied to keep the eye hydrated and to maintain good electrical contact. ERGs were recorded using a Ganzfeld bowl (Ganzfeld QC450 SCX, Roland Consult, Brandenburg, Germany) and an amplifier with a recording unit (RETI-Port, Roland Consult). ERGs were recorded from both eyes simultaneously, band-pass filtered (1 to 300 Hz), and averaged. Single flash scotopic (dark adapted) responses to a series of ten LED-flash intensities ranging from − 3.5 to 1.0 log cd.s/m² with an interstimulus interval of 2 up to 20 s for the highest intensity were recorded. Responses were quantified based on mean waveform peak amplitude and implicit time. All analysis and plotting were carried out with R 3.2.1 (The R Foundation for Statistical Computing) and ggplot2 2.1.0.

Mice were dark adapted for at least 12 hr before recordings and anesthetized by subcutaneous injection of ketamine (65 mg/kg) and xylazine (13 mg/kg). Pupils were dilated with tropicamide eyedrops (Mydriaticum Stulln; Pharma Stulln). Silver needle electrodes served as reference (forehead) and ground (tail) and gold wire ring electrodes as active electrodes. Corneregel (Bausch & Lomb, Berlin, Germany) was applied to keep the eye hydrated and to maintain good electrical contact. ERGs were recorded using a Ganzfeld bowl (Ganzfeld QC450 SCX, Roland Consult, Brandenburg, Germany) and an amplifier with a recording unit (RETI‐Port, Roland Consult). ERGs were recorded from both eyes simultaneously, band‐pass filtered (1–300 Hz) and averaged. Single flash scotopic (dark adapted) responses to a series of ten LED‐flash intensities ranging from −3.5 to 1.0 log cd.s/m² with an interstimulus interval of 2 up to 20 s for the highest intensity were recorded. After 10 min of adaptation to a white background illumination (20 cd/m²) single flash photopic (light adapted) responses to three Xenon‐flash intensities (1, 2, and 3 log cd.s/m²) were recorded. Responses were quantified based on mean waveform peak amplitude and implicit time. All analysis and plotting was carried out with R 3.2.1 (The R Foundation for Statistical Computing) and ggplot2 2.1.0.