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Ezinfo software 3

Manufactured by Sartorius
Sourced in Sweden

EZinfo software 3.0.3 is a data analysis tool developed by Sartorius. It is used for the processing and visualization of analytical data, particularly from chromatography and electrophoresis experiments.

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Lab products found in correlation

3 protocols using ezinfo software 3

1

MS Data Processing and PCA Analysis

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All MSE data were collected and processed using UNIFI 1.8 (Waters Corp., Milford, MA, USA). Data within UNIFI 1.8 is passed through the apex peak detection and alignment processing algorithms. The intensity of each ion was normalized with respect to the total ion count to generate a data matrix having RT, m/z value, and the normalized peak area. Charged species, salt adducts, and fragments are all automatically aligned and grouped. The three-dimensional data including peak number (RT-m/z pair), sample name, and normalized peak areas were exported to the EZinfo software 3.0.3 (UMETRICS, Umea, Sweden) for PCA. The data were mean-centered and Pareto-scaled prior to PCA.
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2

MSE Data Processing for Metabolomics Analysis

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All MSE data were collected and processed using UNIFI 1.8 (Waters Corp., Milford, MA, USA). Data within UNIFI 1.8 were passed through the apex peak detection and alignment processing algorithms. The intensity of each ion was normalized with respect to the total ion count to generate a data matrix having RT, m/z value, and the normalized peak area. Charged species, salt adducts, and fragments were all automatically aligned and grouped. The three-dimensional data, including peak number (RT-m/z pair), sample name, and normalized peak areas, were exported to the EZinfo software 3.0.3 (UMETRICS) for PCA. The processed data were also entered into the MetaboAnalyst website (http://www.metaboanalyst.ca) to perform HCA.
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3

Multivariate Analysis of MS Data

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MSE data were collected and processed using the apex peak detection and alignment algorithms in UNIFI 1.8 (Waters Corp., Milford, MA, USA). The intensity of each ion was normalized by the total ion count to generate a data matrix having RT, m/z value, and the normalized peak area. Charged species, salt adducts, and fragments were automatically aligned and grouped. The three-dimensional data, including peak number (RT-m/z pair), normalized peak areas, and sample name, were then exported to EZinfo software 3.0.3 (UMETRICS) for multivariate analyses such as PCA and OPLS-DA. The data were mean-centered and pareto-scaled before PCA and OPLS-DA.
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