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Lumileds

Manufactured by Philips
Sourced in Netherlands

LumiLEDs is a line of high-performance LED lighting products developed by Philips. These LED lights are designed to provide efficient and reliable illumination for various applications. The core function of LumiLEDs is to generate light through the use of light-emitting diodes (LEDs).

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3 protocols using lumileds

1

Intrinsic Optical Imaging of Whisker Barrel Cortex

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Intrinsic optical imaging was performed through the cranial window as previously described (O'Connor et al., 2010 (link)). Mice were lightly anesthetized with isofluorane (0.5% ) and were placed on a heat blanket with their bodies maintained at 37°. Images were acquired using a CCD camera (Retiga-2000RV, Qimaging, Surrey, BC, Canada) through a Leica MZ12.5 microscope (field of view 4.8 x 3.6 mm) with 630 nm LED illumination (Philips LumiLEDs, Amsterdam, Netherlands). The targeted whisker was placed inside a glass pipette connected to a piezoelectric bimorph. The whisker was deflected at 10 Hz for 4 s every 20 s for 10 min. Images were averaged during the 4 s stimulation epoch. A baseline image of the average of the 10 s period proceeding each stimulation epoch was subtracted of this image to generate the intrinsic signal image. Barrels were visible as regions showing decreased 630 nm reflectance. An image of the vasculature was taken with 530 nm LED illumination (Philips LumiLEDs) as a reference for alignment.
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2

Photodynamic Therapy for Antimicrobial Effects

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A LED (LumiLEDs, Phillips - Netherlands), with a peak wavelength at 660nm, was used as the light source (power output of 100 mW, spot size of 1cm2 and power density of 0.1W/cm2).
Two different energies consisted on 3J and 18J, resulted from 30s and 180s of LED irradiation, were used as sub-lethal and lethal dose of aPDT, respectively [15 (link),22 (link)].
For the in vitro experiments, irradiations were performed from the bottom to the top of the glass test tube and 96 wells plate and for in vivo analysis, larvae were irradiated with a LED (LumiLEDs, Phillips - Netherlands) in a Petri dish plate in a dorsal position.
As a photosensitizer (PS) an aqueous solution of Methylene blue (Sigma Aldrich, Milwaukee, USA) at 100μM were prepared and kept at dark conditions before use.
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3

Wideband Depth Recording and Optogenetic Stimulation

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Wideband (0.3–20kHz, sampled at 40kHz) depth recordings were made using the SciWorks recording system (DataWave Technologies), AM-3600 extracellular amplifiers (A-M Systems), and T8G100 headstage amplifiers (TBSI). Photo stimulation was performed with a custom fiber-coupled LED (Philips Lumileds). LED intensity was controlled using a LED driver with analog modulation (Mightex Systems). Analog output pulses were generated within SciWorks and used to control the LED driver.
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