The deprivation of unilateral cochlea was performed as described previously6 (link). Chicks of P1 were anesthetized with a subcutaneous injection of either chloral hydrate (0.2 g kg−1, Nacalai, Japan) or urethane (0.5 g kg−1, Polysciences, USA), and the unilateral basilar membrane was removed with fine forceps. Following survival periods of 1–28 days, the animals were used for experiments. Most experiments were conducted in neurons with a high tuning frequency (2.5–4 kHz).
Chloral hydrate
Manufactured by Nacalai Tesque
Sourced in Japan
Chloral hydrate is a chemical compound used as a laboratory reagent. It is a colorless crystalline solid that is soluble in water, alcohol, and other organic solvents. Chloral hydrate is commonly used in various chemical and biochemical applications, such as synthesis, analysis, and preservation.
Automatically generated - may contain errors
Lab products found in correlation
Formalin neutral buffer solution, by Fujifilm (2 mentions)
Eosin alcohol solution, by Muto Pure Chemicals (2 mentions)
Malinol, by Muto Pure Chemicals (2 mentions)
Diff quik, by Sysmex (2 mentions)
Mayer s hematoxylin, by Muto Pure Chemicals (2 mentions)
Porcine pancreatic elastase, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Icr mice, by Charles River Laboratories (1 mentions)
Methylcellulose, by Fujifilm (1 mentions)
Potassium dihydrogen phosphate, by Fujifilm (1 mentions)
Mepenzolate, by Merck Group (1 mentions)
Dmi3000b, by Leica (1 mentions)
Lsm 700, by Zeiss (1 mentions)
Safranin o, by Leica (1 mentions)
Safranin o, by Fujifilm (1 mentions)
Propofol, by Merck Group (1 mentions)
Riluzole, by Merck Group (1 mentions)
Mk 801 hydrogen maleate, by Merck Group (1 mentions)
Ketamine hydrochloride, by Daiichi Sankyo (1 mentions)
Mouse anti cyclin d1, by Santa Cruz Biotechnology (1 mentions)
8 protocols using chloral hydrate
1
Unilateral Cochlea Deprivation in Chicks
2
Porcine Pancreatic Elastase and Methacholine Chloride Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Porcine pancreatic elastase (PPE) and methacholine chloride (methacholine) were obtained from Sigma-Aldrich (St. Louis, MO, United States). Novo-Heparin for injection was from Mochida Pharmaceutical, Co. (Tokyo, Japan). Chloral hydrate was from Nacalai Tesque (Kyoto, Japan). Diff-Quik was from Sysmex, Co. (Kobe, Japan). Formalin neutral buffer solution was from WAKO Pure Chemicals (Tokyo, Japan). Mayer’s hematoxylin, 1% eosin alcohol solution and malinol were from MUTO Pure Chemicals (Tokyo, Japan).
3
Analysis of Mepenzolate in Mouse Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mepenzolate, PPE and HPLC-grade acetonitrile were obtained from Sigma-Aldrich (St. Louis, MO). Novo-Heparin for injection was from Mochida Pharmaceutical Co. (Tokyo, Japan). Chloral hydrate was from Nacalai Tesque (Kyoto, Japan). Diff-Quik was from the Sysmex Co (Kobe, Japan). Sodium 1-propanesulfonate was from Tokyo Kasei Chemical Co (Tokyo, Japan). The Amicon utra-0.5 centrifugal filter unit was purchased from Merck Millipore (Billerica, MA). Formalin neutral buffer solution, potassium dihydrogen phosphate and methylcellulose were from WAKO Pure Chemicals (Tokyo, Japan). Mayer's hematoxylin, 1% eosin alcohol solution and malinol were from MUTO Pure Chemicals (Tokyo, Japan). ICR mice (4–6 weeks old, male) were purchased from Charles River (Yokohama, Japan). The experiments and procedures described here were carried out in accordance with the Guide for the Care and Use of Laboratory Animals as adopted and promulgated by the National Institutes of Health, and were approved by the Animal Care Committee of Keio University.
4
Xylem Cell Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For xylem cell staining, haustoria were excised from roots, immersed in 10% (w/v) KOH (Wako Chemicals, Osaka, Japan) for 15 min at 90°C, rinsed three times with water, immersed in 0.1% (w/v) Safranin O (Wako Chemicals, Osaka, Japan) solution for 5 min at 90°C, and rinsed three times with water. The stained haustoria were cleared by soaking samples in clearing solution [chloral hydrate (2.5 g ml−1) (Nacalai Tesque), 33% (v/v) glycerol] from 3 hours to overnight before observation. Safranin O–stained xylem cells were observed with a light microscope (Leica DMI3000 B) and an LSM700 laser-scanning confocal microscope (Carl Zeiss) with excitation and emission wavelength suitable for fluorescent images of red fluorescent protein (RFP).
5
Telemetric Measurement of Oscillations in mPFC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In order to measure δ- (0.5–4 Hz), θ- (4–8 Hz), α- (8–12 Hz), σ- (12–16 Hz), β- (16–24 Hz), and γ- (25–55 Hz) oscillations in the mPFC, we used a telemetry implant (PhysioTel® transmitter TA10ETA-F20; DSI, Lexington, KY, USA) and a receiver (RPC-1, DSI). Mice were anesthetized with chloral hydrate (400 mg/kg) (Nacalai Tesque Inc., Kyoto, Japan). An electric probe was stereotaxically inserted into layer V within the prelimbic and infralimbic areas of mPFC (Bregma: 1.9 mm anterior, 0.5 mm lateral [right], 3.5 mm ventral) and the other electrode was placed in the longitudinal cerebral fissure (Bregma: 6 mm posterior, 0-mm lateral). DataquestTM A.R.T.TM (DSI) software was used to acquire and analyze EEG data. EEGs were recorded during the habituation period, in which mice were placed in a novel cage for 30 min, and during subsequent social interaction, in which an age-matched male mouse was inserted into the cage for 5 min. The ratio of oscillation power during social interaction (per 5 min) to the average of oscillation power during habituation (per 5 min) was measured to evaluate mPFC function in response to social interaction. The randomization of mice for examination was performed as in the IEG response experiments (Fig. 1b ). This experiment was performed in darkness during the dark period.
6
Pharmacological Modulators of Neurological Function
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
(+)-MK-801 hydrogen maleate, riluzole, and propofol were purchased from Sigma-Aldrich Co., LLC., (±)ketamine hydrochloride from Daiichi Sankyo Propharma Co., Ltd., haloperidol from Sumitomo Pharma Co., Ltd., chloral hydrate from Nacalai Tesque, Inc, and xylazine hydrochloride from FUJIFILM Wako Chemicals Co., Ltd. Mepivacaine hydrochloride was obtained from Nippon Shika Yakuhin Co., Ltd. All drugs, except for propofol and mepivacaine, were dissolved in 0.9% saline solution. propofol was prepared in 10% intralipid as an emulsion. Each drug was freshly prepared on the day of the experiment. (+)-MK-801, (±)-ketamine, riluzole, and propofol were intraperitoneally (i.p.) administered at a volume of 0.1 mL per 10 g of body weight. Haloperidol was injected i.p. 30 minutes before the administration of (+)-MK-801 at a volume of 5 mL/kg.
7
Investigating Hypothalamus and Adipose Tissue Changes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were deeply anesthetized with chloral hydrate (400 mg/kg, i.p., Nacalai Tesque) and sacrificed. Mouse whole brain was quickly removed 24 hours after the last ECS or sham treatment, and a coronal slice was made between bregma −1.22 mm and −2.06 mm.17 The mediobasal hypothalamus, including the VMH, was then excised under a microscope. Epididymal white adipose tissue (eWAT) was collected and weighed. Both samples were immediately frozen on dry ice and stored at −20°C until further processing.
8
Antibody-Based Analysis of Centrosomal Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies used in this study include mouse anti-glutamylated tubulin (GT335) (1:1000, Adipogen, AG-20B-0020), rabbit anti-ARL13B (1:1000, Proteintech, 17711-1-AP), mouse anti-ARL13B (1:1000, NeuroMab, 75-287), mouse anti-CEP164 (1:500, Santa Cruz, sc-515403), rabbit anti-Ki67 (1:2000, Abcam, ab15580), rabbit anti-TTBK2 (1:500, Sigma-Aldrich, HPA018113), rabbit anti-CP110 (1:1000, gift from B. D. Dynlacht), rabbit anti-KRAS (1:2000, Proteintech, 12063-1-AP), rabbit anti-CDK4 (1:400, Santa Cruz, sc-260), mouse anti-CDK6 (1:400, Santa Cruz, sc-7961), mouse anti-Cyclin D1 (1:400, Santa Cruz, sc-8396), mouse anti-Cyclin D2 (1:400, Santa Cruz, sc-53637), mouse anti-Cyclin D3 (1:400, Santa Cruz, sc-6283), goat anti-GLI2 [1:100 (IF), 1:400 (WB), Santa Cruz, sc-271786], and mouse anti-β-Actin (1:1000, Santa Cruz, sc-47778). Reagents used in this study include Chloral Hydrate (Nacalai Tesque, 07922-62), Hoechst 33342 (Nacalai Tesque, 04915-82), and Propidium Iodide (PI) (Nacalai Tesque, 29037-76).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!