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Kras pyro kit

Manufactured by Qiagen
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The KRAS Pyro Kits are a line of products designed for the detection and quantification of mutations in the KRAS gene. These kits utilize pyrosequencing technology to analyze specific regions of the KRAS gene and identify the presence and percentage of common mutations.

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Lab products found in correlation

Qiaamp circulating nucleic acid kit, by Qiagen (1 mentions)

Spelling variants of this product

Therascreen KRAS Pyro Kit (16 citations) Therascreen KRAS Pyro Kit Handbook (3 citations)

4 protocols using kras pyro kit

1

Screening for BRAF, KRAS, and TP53 Mutations

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Mutations within codon 600 of BRAF and codons 12 and 13 of KRAS were examined by pyrosequencing using BRAF and KRAS pyro kits (Qiagen) according to the manufacturer's instructions [2 (link)]. Mutation of TP53 was initially determined by PCR-SSCP followed by direct sequencing, as described [2 (link)].
Kamimae S., Yamamoto E., Kai M., Niinuma T., Yamano H.O., Nojima M., Yoshikawa K., Kimura T., Takagi R., Harada E., Harada T., Maruyama R., Sasaki Y., Tokino T., Shinomura Y., Sugai T., Imai K, & Suzuki H. (2015). Epigenetic silencing of NTSR1 is associated with lateral and noninvasive growth of colorectal tumors. Oncotarget, 6(30), 29975-29990.
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2

Genetic Profiling of BRAF, KRAS, and TP53

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Mutations within codon 600 of BRAF and codons 12 and 13 of KRAS were examined by pyrosequencing using BRAF and KRAS pyro kits (Qiagen) according to the manufacturer's instructions. TP53 mutation was initially assessed using PCR-SSCP followed by direct sequencing, as described previously [7 (link)].
Aoki H., Yamamoto E., Takasawa A., Niinuma T., Yamano H.O., Harada T., Matsushita H.O., Yoshikawa K., Takagi R., Harada E., Tanaka Y., Yoshida Y., Aoyama T., Eizuka M., Yorozu A., Kitajima H., Kai M., Sawada N., Sugai T., Nakase H, & Suzuki H. (2017). Epigenetic silencing of SMOC1 in traditional serrated adenoma and colorectal cancer. Oncotarget, 9(4), 4707-4721.
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3

Detecting KRAS Mutations in Pancreatic Juice

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Pancreatic juice secretion was stimulated by infusing synthetic secretin (Secrulux®, Sanochemia Diagnostic, Neuss, Germany) and was then collected from the duodenal lumen. Circulating DNA was isolated by using the QIAamp® Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany) and was tested for the presence of mutant KRAS by using the therascreen®KRAS Pyro® Kit (Qiagen), according to the manufacturer’s instructions.
Bartsch D.K., Gercke N., Strauch K., Wieboldt R., Matthäi E., Wagner V., Rospleszcz S., Schäfer A., Franke F.S., Mintziras I., Bauer C., Grote T., Figiel J., Di Fazio P., Burchert A., Reinartz S., Pogge von Strandmann E., Klöppel G, & Slater E.P. (2018). The Combination of MiRNA-196b, LCN2, and TIMP1 is a Potential Set of Circulating Biomarkers for Screening Individuals at Risk for Familial Pancreatic Cancer. Journal of Clinical Medicine, 7(10), 295.
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4

KRAS Mutation Analysis by Pyrosequencing

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KRAS gene mutations were assessed by pyrosequencing using the KRAS Pyro Kit (Qiagen, Inc.) according to manufacturer's protocol. Five microliters of extracted DNA was amplified for sequencing. A 15-µl aliquot of each PCR product was used for pyrosequencing to analyse KRAS codons 12, 13 and 61.
Gebrekiristos M., Melson J., Jiang A, & Buckingham L. (2022). DNA methylation and miRNA expression in colon adenomas compared with matched normal colon mucosa and carcinomas. International journal of experimental pathology, 103(3).
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