St (99.0%), azobisisobutyronitrile (AIBN) (98.0%) and PBS buffer solution (×10) were purchased from Wako Pure Chemical Industry Ltd., Osaka, Japan. Super dehydrated toluene (99.5%), divinylbenzene (DVB) (50.0%), 4-vinylpyridine (4VP) (95.0%), Sodium 4-styrenesulfonate (93.0%), Acetonitrile (99.0%) and isobutylphenyl propionic acid (Ibuprofen,> 98.0%) were all purchased from Tokyo Chemical Industry Co., Ltd., Tokyo, Japan (TCI). d -limonene (90.0%), tetrahydrofuran (98.0%), dimethyl sulfoxide (99.0%) and bromocresol purple were purchased from Nacalai Tesque Inc. Kyoto Japan. Rhodamine B was purchased from Sigma Aldrich Co., St.Louis, MO, USA. Vinylbenzyl trimethylammonium chloride (VBAC) was purchased from Santa Cruz Biotechnology, Kyoto, Japan. All chemicals were used as supplied without further purification, except where noted otherwise.
D limonene
Manufactured by Nacalai Tesque
Sourced in Japan
D-limonene is a naturally occurring organic compound found in the rinds of citrus fruits. It is a colorless liquid with a distinct citrus aroma. D-limonene is commonly used as a solvent and as a fragrance in various products.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Nacalai Tesque (1 mentions)
Azobisisobutyronitrile, by Fujifilm (1 mentions)
Tetrahydrofuran, by Nacalai Tesque (1 mentions)
Divinylbenzene dvb, by Tokyo Chemical Industry (1 mentions)
Acetonitrile, by Tokyo Chemical Industry (1 mentions)
Rhodamine b, by Merck Group (1 mentions)
Crl cd sd rats, by Charles River Laboratories (1 mentions)
Crf 1, by Oriental Yeast (1 mentions)
Corn oil, by Nacalai Tesque (1 mentions)
Chitosan, by Tokyo Chemical Industry (1 mentions)
D limonene, by Thermo Fisher Scientific (1 mentions)
2 propanol, by Kanto Chemical (1 mentions)
Noa 81, by Norland Products (1 mentions)
Chloroform, by Fujifilm (1 mentions)
Methyl acetate, by Fujifilm (1 mentions)
Ethanol, by Fujifilm (1 mentions)
6 protocols using d limonene
1
Monomer Synthesis and Polymerization for Biomaterial Development
2
Rat Model of α2u-Globulin Nephropathy
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A total of 18 male Crl(CD):SD rats (Charles River Laboratories Japan Inc., Kanagawa, Japan) were obtained at 10 weeks of age and used at 11 weeks. d-Limonene (Nacalai Tesque Inc., Kyoto, Japan), a well-known α2u-globulin nephropathy inducer, was administered to groups of rats consisting of 3 males each at 300 mg/kg/day by gavage using corn oil (Nacalai Tesque Inc.) as a vehicle for 1, 5, 10, or 20 days. Control animals consisting of 3 males each were dosed with the vehicle for 1 or 20 days (Supplementary Table 1 : online only). The rats were housed individually in stainless steel wire cages in an animal room maintained at 24 ± 2°C and 55 ± 10% humidity with a 12-hr light/dark cycle (lighting from 7:00 to 19:00) and were allowed free access to food (CRF-1, Oriental Yeast Co., Ltd., Tokyo, Japan) and water. This study was conducted in accordance with the principles outlined in the Guide for the Care and Use of Laboratory Animals of the Japanese Association for Laboratory Animal Science and those of our institution.
3
Nano-Encapsulation of d-Limonene
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d ‐limonene (ρ = 0.84g/cm3 at 20°C, Molar Mass: 136.24 g/mol) was purchased from Nacalai Tesque. Gum Arabic and Maltodextrin (DE = 16.5–19.5) were supplied from SDFCL and Sigma‐Aldrich, respectively. Sugar was prepared from Paniz Shahd Binalood Co. Citric acid was obtained from Sigma‐Aldrich Chemistry. Distilled water was used for preparing all solutions. All organic chemicals used in the analyses were of the analytical grade.
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4
Chitosan-Based Coatings and Limonene Emulsions
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Chitosan solution (CS) was made by dissolving 5 g Chitosan (Tokyo Chemical Industry Co. Ltd., Tokyo, Japan) in 1 L 0.5% acetic acid. To prepare Chitosan coating incorporated with PE, 10 or 20 mL PE was mixed with 1 L CS, and the solution was stirred at room temperature for 3 h. To prepare d-limonene emulsion, 1 mL polyoxyethylene sorbitan monooleate (Tween®80; Nacalai Tesque Inc., Kyoto, Japan) was mixed with 1 mL ultrapure water or d-limonene (Alfa Aesar, Ward Hill, MA, USA), the mixture was added dropwise into 1 L ultrapure water with constant stirring and homogenized for 3 h at room temperature.
5
Fabrication of Gold Nanostructures via NIL
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The gold nanostructures were fabricated using the NIL process (Figure S1 ). In this study, a cyclo-olefin polymer (COP) film (FLH230/200-120, Scivax Co., Ltd., Kanagawa, Japan) was used as the mold. We selected this structure herein because it has been previously reported by Nishiguchi et al. that plasmon excitation is possible [25 (link)]. Initially, the COP mold was cleaned with 2-propanol (Kanto Chemical Co. Inc., Tokyo, Japan) and ultrapure water, and then dried by airflow. A 200-nm-thick Au layer was thermally deposited onto the COP mold. The deposited Au layer was attached to the surface of the QCM (SEN-5P-H-10; TAMADEVICE Co., Ltd., Kanagawa, Japan) using a photocurable polymer (NOA81; Norland Products Inc., Cranbury, NJ, USA), followed by dissolution of the COP mold in D-limonene (NACALAI TESQUE, Inc., Kyoto, Japan), after which a QCM device with a gold nanostructure was obtained.
6
Synthesis and Characterization of CER
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The synthetic D-erythro-ceramide ［NDS］ 16) , CER, was obtained from Takasago International Corp. (Tokyo, Japan) . According to the supplier, both chemical and optical purities of CER are more than 95％. acResin ® A260UV was used as the adhesive material. The prepolymers with a mean molecular weight of -200,000 were supplied by BASF (Ludwigshafen am Rhein, Germany) . The acResin ® A260UV is made from benzophenone groups and the polymer of butyl acrylate, according to the information of BASF s product catalogue. D-Limonene (purity＞90％) was obtained from Nacalai Tesque, Inc. (Kyoto, Japan) , and methyl acetate (purity＞98％) , chloroform (purity＞99％) , and ethanol (purity＞99.5％) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan) . All chemicals were of reagent grade and were used as they were received without further purification.
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