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T 5224

Manufactured by Cayman Chemical
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Sourced in Germany

T-5224 is a laboratory instrument designed for the purification and analysis of chemical compounds. It utilizes high-performance liquid chromatography (HPLC) technology to separate and identify the components of a given sample. The core function of T-5224 is to provide precise and reliable data for researchers and scientists working in the chemical, pharmaceutical, and related industries.

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Lab products found in correlation

Glutathione (gsh), by Selleck Chemicals (2 mentions) Bosutinib, by Merck Group (2 mentions) Cobimetinib, by Selleck Chemicals (2 mentions) Ro31 8220, by Merck Group (2 mentions) 1 2 dithiole 3 thione, by Merck Group (2 mentions) Bpiqii, by Cayman Chemical (2 mentions) Sr11302, by Cayman Chemical (2 mentions) Marimastat, by Santa Cruz Biotechnology (2 mentions) 3 3 4 4 5 pentachlorobiphenyl pcb126, by LGC (2 mentions) 2 3 4 4 5 pentachlorobiphenyl pcb118, by LGC (2 mentions) Prostaglandin d2, by Thermo Fisher Scientific (2 mentions) Ch 223191, by Selleck Chemicals (2 mentions) Mg132, by Merck Group (2 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions) Flufenamic acid, by Merck Group (2 mentions) Hydrogen peroxide, by Merck Group (2 mentions) Benzo a pyrene, by Merck Group (2 mentions) Recombinant mouse il 6, by R&D Systems (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions) Bay 11 7085, by Cayman Chemical (1 mentions)

10 protocols using t 5224

1

Investigating A549 Cell Responses to COX-2, AP-1, and NF-κB Inhibitors

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A549 cells were seeded in individual 12-well plates at a density of 1 × 105 cells/well and were allowed to grow overnight. The next morning celebrex, BAY 11-7085 (BAY 11), or T-5224 were administered in complete medium at the indicated concentrations; Celecoxib (Cayman Chemical) was used to inhibit COX-2 activity, T-5224 (Cayman Chemical) was used to inhibit AP-1 binding to DNA, and BAY 11 (Cayman Chemical) was used to inhibit NF-κB activation. DMSO alone was used as the control treatment. RNA was isolated 6–24 hours after treatment.
Desind S.Z., Iacona J.R., Yu C.Y., Mitrofanova A, & Lutz C.S. (2022). PACER lncRNA regulates COX-2 expression in lung cancer cells. Oncotarget, 13, 291-306.
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2

Neuronal Growth Factor Signaling

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DG2 and mouse NGF was supplied by Millipore-Sigma (Burlington, MA, USA); T-5224 and PGE2 by Cayman Chemicals (Ann Arbor, MI, USA). The recombinant mouse IL-6 was supplied by R&D Systems (Minneapolis, MN, USA).
de la Peña J.B., Kunder N., Lou T.F., Chase R., Stanowick A., Barragan-Iglesias P., Pancrazio J.J, & Campbell Z.T. (2021). A role for translational regulation by S6 kinase and a downstream target in inflammatory pain. British journal of pharmacology, 178(23), 4675-4690.
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3

Cardiomyocyte Luciferase Assay

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Primary adult rat ventricular cardiomyocytes (5 × 104 per well) were cultured in a 6-well plate and treated with luciferase adenovirus or with 20 μM T-5224 (Cayman Chemical, catalog 22904) the same day. After 24 hours of culture, phenylephrine (PE) at a final concentration of 10 μM was added and cultured for another 24 hours until assay. The luciferase activity was determined using the dual-luciferase reporter assay system (Promega, catalog E1910). The ratio of firefly to Renilla luciferase activity was calculated.
Zhou B., Caudal A., Tang X., Chavez J.D., McMillen T.S., Keller A., Villet O., Zhao M., Liu Y., Ritterhoff J., Wang P., Kolwicz SC J.r., Wang W., Bruce J.E, & Tian R. (2022). Upregulation of mitochondrial ATPase inhibitory factor 1 (ATPIF1) mediates increased glycolysis in mouse hearts. The Journal of Clinical Investigation, 132(10), e155333.
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4

Diverse Compound Treatments in Cell Assays

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Benzo[a]pyrene, benzo[k]fluoranthene, bosutinib, 1,2-dithiole-3-thione, flufenamic acid, MG132, PP2, Ro-31–8220 and hydrogen peroxide were purchased from Sigma-Aldrich (Taufkirchen, Germany), BPIQII, SR11302 and T-5224 from Cayman Chemicals (Ann Arbor, MI), and CH223191, cobimetinib and glutathione from Selleckchem (Houston, TX). Marimastat was purchased from Santa Cruz Biotechnology (Dallas, TX) and PD153035 from Absource Diagnostics (Munich, Germany). 3,3′,4,4′,5-Pentachlorobiphenyl (PCB126) and 2,3′,4,4′,5-pentachlorobiphenyl (PCB118) were bought from LGC Standards (Wesel, Germany) and 2,3,7,8-tetrachlorodibenzo-p-dioxin from Amchro (Hattersheim am Main, Germany). Prostaglandin D2, AREG, TGFα and EGF were purchased from PeproTech (Rocky Hill, NY). hydrogen peroxide, glutathione and the three EGFR ligands were dissolved or diluted in water, the other compounds in DMSO. Treatment time and applied concentrations of the chemicals and human recombinant proteins is indicated in the figure legends.
Vogeley C., Sondermann N.C., Woeste S., Momin A.A., Gilardino V., Hartung F., Heinen M., Maaß S.K., Mescher M., Pollet M., Rolfes K.M., Vogel C.F., Rossi A., Lang D., Arold S.T., Nakamura M, & Haarmann-Stemmann T. (2021). Unraveling the differential impact of PAHs and dioxin-like compounds on AKR1C3 reveals the EGFR extracellular domain as a critical determinant of the AHR response. Environment international, 158, 106989.
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5

Diverse Compound Treatments in Cell Assays

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Benzo[a]pyrene, benzo[k]fluoranthene, bosutinib, 1,2-dithiole-3-thione, flufenamic acid, MG132, PP2, Ro-31–8220 and hydrogen peroxide were purchased from Sigma-Aldrich (Taufkirchen, Germany), BPIQII, SR11302 and T-5224 from Cayman Chemicals (Ann Arbor, MI), and CH223191, cobimetinib and glutathione from Selleckchem (Houston, TX). Marimastat was purchased from Santa Cruz Biotechnology (Dallas, TX) and PD153035 from Absource Diagnostics (Munich, Germany). 3,3′,4,4′,5-Pentachlorobiphenyl (PCB126) and 2,3′,4,4′,5-pentachlorobiphenyl (PCB118) were bought from LGC Standards (Wesel, Germany) and 2,3,7,8-tetrachlorodibenzo-p-dioxin from Amchro (Hattersheim am Main, Germany). Prostaglandin D2, AREG, TGFα and EGF were purchased from PeproTech (Rocky Hill, NY). hydrogen peroxide, glutathione and the three EGFR ligands were dissolved or diluted in water, the other compounds in DMSO. Treatment time and applied concentrations of the chemicals and human recombinant proteins is indicated in the figure legends.
Vogeley C., Sondermann N.C., Woeste S., Momin A.A., Gilardino V., Hartung F., Heinen M., Maaß S.K., Mescher M., Pollet M., Rolfes K.M., Vogel C.F., Rossi A., Lang D., Arold S.T., Nakamura M, & Haarmann-Stemmann T. (2021). Unraveling the differential impact of PAHs and dioxin-like compounds on AKR1C3 reveals the EGFR extracellular domain as a critical determinant of the AHR response. Environment international, 158, 106989.
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6

Reagents and Knockdown Methods for Wnt3A Signaling

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17β-estradiol (E2, #E8875) was obtained from Sigma, and Fulvestrant (#1047), carbenoxolone disodium (#3096) and EDTA (#2811) were purchased from Tocris. LGK974 (#14072) and T-5224 (#22904) were purchased from Cayman. Marimastat (S7156) was obtained from SelleckChem. Recombinant human Wnt3A (5036-WN-010) was purchased from R&D Systems. For knockdown experiments, siRNA against FOXA1 (#M-010319), DSC1 (#L-011995), DSC2 (#L-011996), GJA1 (#L-011042) and GJB2 (#L-019285) were obtained from Horizon Discovery. Desmosome and scramble peptides were designed based on previous studies (21 ,22 (link)) and synthesized from GeneScript. Peptide sequences are presented in Supplementary Table S10.
Li Z., Wu Y., Yates M.E., Tasdemir N., Bahreini A., Chen J., Levine K.M., Priedigkeit N.M., Nasrazadani A., Ali S., Buluwela L., Arnesen S., Gertz J., Richer J.K., Troness B., El-Ashry D., Zhang Q., Gerratana L., Zhang Y., Cristofanilli M., Montanez M.A., Sundd P., Wallace C.T., Watkins S.C., Fumagalli C., Guerini-Rocco E., Zhu L., Tseng G.C., Wagle N., Carroll J.S., Jank P., Denkert C., Karsten M.M., Blohmer J.U., Park B.H., Lucas P.C., Atkinson J.M., Lee A.V, & Oesterreich S. (2022). Hotspot ESR1 mutations are multimodal and contextual modulators of breast cancer metastasis. Cancer research, 82(7), 1321-1339.
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7

Inhibition of c-Fos/AP-1 Pathway

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T-5224 (c-Fos/AP-1inhibitor, Cayman Chemicals, #22904) was dissolved in DMSO and stored at - 80°C. DMSO was always added in the same proportions to control wells.
Kuppe C., Ibrahim M.M., Kranz J., Zhang X., Ziegler S., Perales-Patón J., Jansen J., Reimer K.C., Smith J.R., Dobie R., Wilson-Kanamari J.R., Halder M., Xu Y., Kabgani N., Kaesler N., Klaus M., Gernhold L., Puelles V.G., Huber T.B., Boor P., Menzel S., Hoogenboezem R.M., Bindels E.M., Steffens J., Floege J., Schneider R.K., Saez-Rodriguez J., Henderson N.C, & Kramann R. (2020). Decoding myofibroblast origins in human kidney fibrosis. Nature, 589(7841), 281-286.
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8

JUN Modulation in Adhesion Surgery

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Doxycycline (2 mg/mL) or T-5224 (Cayman Chem, 10uM) resuspended in DMSO were applied locally to adhesion sites at the time of adhesion surgery for JUN induction (in JUN mice) or suppression (in JUN and WT mice), respectively. Vehicle only was used for control.
Foster D.S., Marshall C.D., Gulati G.S., Chinta M.S., Nguyen A., Salhotra A., Jones R.E., Burcham A., Lerbs T., Cui L., King M.E., Titan A.L., Ransom R.C., Manjunath A., Hu M.S., Blackshear C.P., Mascharak S., Moore A.L., Norton J.A., Kin C.J., Shelton A.A., Januszyk M., Gurtner G.C., Wernig G, & Longaker M.T. (2020). Elucidating the fundamental fibrotic processes driving abdominal adhesion formation. Nature Communications, 11, 4061.
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9

Inhibition of c-Fos/AP-1 Pathway

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T-5224 (c-Fos/AP-1inhibitor, Cayman Chemicals, #22904) was dissolved in DMSO and stored at - 80°C. DMSO was always added in the same proportions to control wells.
Kuppe C., Ibrahim M.M., Kranz J., Zhang X., Ziegler S., Perales-Patón J., Jansen J., Reimer K.C., Smith J.R., Dobie R., Wilson-Kanamari J.R., Halder M., Xu Y., Kabgani N., Kaesler N., Klaus M., Gernhold L., Puelles V.G., Huber T.B., Boor P., Menzel S., Hoogenboezem R.M., Bindels E.M., Steffens J., Floege J., Schneider R.K., Saez-Rodriguez J., Henderson N.C, & Kramann R. (2020). Decoding myofibroblast origins in human kidney fibrosis. Nature, 589(7841), 281-286.
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10

Modulating NF-κB and AP-1 Pathways

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LPS purified from E coli (serotype O55:B5, Cat# L2880), 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA, Cat # P1585) and Progesterone (P4, Cat # P8783) were purchased from Sigma‐Aldrich. The NF‐кB inhibitor: JSH (cat #ab144824) was obtained from Abcam, and AP‐1 inhibitor: T5224 (Cat #22904) from Cayman Chemicals. LPS was reconstituted with ultrapure water, P4 with 70% ethanol, while TPA, JSH and T5224 were reconstituted with dimethyl sulphoxide (DMSO) as per manufacturer's instructions.
Nadeem L., Balendran R., Dorogin A., Mesiano S., Shynlova O, & Lye S.J. (2021). Pro‐inflammatory signals induce 20α‐HSD expression in myometrial cells: A key mechanism for local progesterone withdrawal. Journal of Cellular and Molecular Medicine, 25(14), 6773-6785.
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