Plasma concentration of cell-free Hb was evaluated using Plasma/Low Hb (Hemocue, Ängelholm, Sweden) and human Hb ELISA according to instructions from the manufacturer (Genway Biotech Inc., San Diego, Ca, USA). Ability to discriminate between cell-free rat Hb and cell-free human Hb was confirmed by analysis of plasma from animals not subjected to i.p. injection of human cell-free Hb.
Plasma low hb
Manufactured by HemoCue
Sourced in Sweden
The Plasma/Low Hb is a lab equipment product designed to measure hemoglobin levels in plasma samples. It provides accurate and reliable results for hemoglobin concentration analysis.
Automatically generated - may contain errors
4 protocols using plasma low hb
1
Quantifying Cell-Free Hemoglobin Levels
2
Purification of Human Cell-Free Hemoglobin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human cell-free Hb was purified as previously described [25 (link)] from human adult red blood cells obtained from the blood center in Lund (Sweden). The Hb concentration was quantified using Plasma/Low Hb (Hemocue, Ängelholm, Sweden). The Hb was dissolved in Ringer’s Acetate (Baxter, Deerfield, Ill, USA) and purified from endotoxin contamination using EndoTrap as described by the manufacturer (Hyglos GmbH, Germany). The absolute purity of Hb from contamination with endotoxin was determined by QCL-1000™ Endpoint Chromogenic LAL Assay as described by the manufacturer (Lonza, Switzerland).
3
Preterm IVH Cerebrospinal Fluid Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CSF was sampled from four preterm infants (gestational age at birth 25 to 28 weeks) at 6 to 11 days after detection of IVH, by spinal tap or ventricular reservoir puncture according to clinical routine in the neonatal unit at Lund University Hospital. Immediately after sampling, the CSF was centrifuged (2,000 × g, 20°C for 10 minutes), pooled and Hb- and Hb-metabolite (that is oxyHb and metHb) concentrations were determined in CSF from preterm infants using Plasma/Low Hb (Hemocue, Ängelholm, Sweden) and a spectrophotometric method described previously [20 (link)]. Samples were stored at -80°C until further use, as described below. The sampling was performed following written consent from the parents, and the study was approved by the ethical committee review board for studies in human subjects at Lund University.
4
Standardized Blood Collection and Platelet Testing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was collected for the anticoagulant CPDA-1 from healthy donors who had not taken anticoagulants, including acetylsalicylic acid, clopidogrel, or warfarin/acenocumarol, for at least two weeks. After collection, the blood was stored for up to 24 h at 2–7 °C. Before further tests, the following parameters were checked: blood count (BC 2800 VET hematology automaton, Mindray, Shenzhen, China), platelet aggregation under adenosine diphosphate (ADP) and arachidonic acid (multiplate impedance aggregometer, Roche, Switzerland, with ADPtest and ASPItest tests), and the concentration of plasma-free hemoglobin (fHB) (spectrophotometer Plasma/Low Hb, Hemocue AB, Ängelholm, Sweden). The following conditions were fulfilled in order to qualify blood for further tests: the blood count was normal, the platelet count was >120 × 103 L/uL, the ADP test result was >122 AUC, the ASPI test result was >136 AUC, and the fHB < 0.2 g/dL.
Platelet-rich plasma (PRP) [18 (link)] was prepared immediately prior to testing via the centrifugation of whole blood at 100 G for 10 min at room temperature. The plasma morphology of the PRP was examined prior to its use in the experiment.
Platelet-rich plasma (PRP) [18 (link)] was prepared immediately prior to testing via the centrifugation of whole blood at 100 G for 10 min at room temperature. The plasma morphology of the PRP was examined prior to its use in the experiment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!