Ap1208

Manufactured by ABclonal

Sourced in China

AP1208 is a Protein A Agarose resin designed for the purification of antibodies and other proteins containing the Fc region. The resin is composed of highly cross-linked agarose beads with covalently coupled recombinant Protein A ligands, which provide high binding capacity and selectivity for immunoglobulins.

Automatically generated - may contain errors

Lab products found in correlation

Bca kit, by Beyotime (1 mentions) Ba1051, by Boster Bio (1 mentions) Ab278545, by Abcam (1 mentions) A11355, by ABclonal (1 mentions) Ap0115, by ABclonal (1 mentions) A5754, by ABclonal (1 mentions) A4992, by ABclonal (1 mentions) Ba1054, by Boster Bio (1 mentions) Enhanced chemiluminescence ecl kit, by Applygen (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Bca protein assay reagent, by Beyotime (1 mentions) Sa00001 1, by Proteintech (1 mentions) Sa00001 2, by Proteintech (1 mentions)

2 protocols using ap1208

Protein Expression Analysis Protocol

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Protein concentrations were determined using a BCA Kit (Beyotime, Beyotime Biotechnology, Shanghai, China). Equal amounts of proteins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes. Antibodies against GPR41 (66811–1-Ig, Sanying, WuHan, China) PI3K (A4992, ABclonal, Wuhan, China), phosphorylated (p)-PI3K (Ab278545, Abcam, UK), Akt (A18675, ABclonal, Wuhan, China), p-Akt (AP1208, ABclonal, Wuhan, China), mTOR (A11355, ABclonal, Wuhan, China), p-mTOR (AP0115, ABclonal, Wuhan, China), RxRα (A19105, ABclonal, Wuhan, China), SREBP1 (A5754, ABclonal, Wuhan, China), PPARγ (A16958, ABclonal, Wuhan, China), St6gal1 (A5754, ABclonal, Wuhan, China), and GAPDH (AB-P-R001, Xianzhi, Hangzhou, China) were used as primary antibodies. All primary antibodies were used at a 1:1000 dilution except for those against p-PI3K (1:500 dilution) and GPR41 (1:2000 dilution). Goat anti-rabbit-HRP (BA1054, Boster Bio, Wuhan, China) and goat anti-mouse-HRP (BA1051, Boster Bio, Wuhan, China) were used as secondary antibodies. The bands were visualized using an Enhanced Chemiluminescence (ECL) Kit (Applygen, Applygen Technologies Co., Ltd., Beijing, China). The protein bands were densitometrically analyzed using the IPP software.

Wang Y., Rui B., Ze X., Liu Y., Yu D., Liu Y., Li Z., Xi Y., Ning X., Lei Z., Yuan J., Li L., Zhang X., Li W., Deng Y., Yan J, & Li M. (2024). Sialic acid-based probiotic intervention in lactating mothers improves the neonatal gut microbiota and immune responses by regulating sialylated milk oligosaccharide synthesis via the gut–breast axis. Gut Microbes, 16(1), 2334967.

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Western Blot Analysis of PI3K/AKT/mTOR Pathway

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Western blot analysis as previously reported [7] . RIPA lysis buffer (Beyotime, Shanghai, China) was used for protein extraction and BCA Protein Assay Reagent (P0012, Beyotime, China) was used for protein concentration determination. 40 μg of protein was used for electrophoresis. PVDF membranes were incubated with primary antibodies against p-PI3K (1:5000, AP1208, Abclonal, China), PI3K (1:1000, 20584-1-AP, Proteintech, China), AKT (1:800, 60203-2-Ig, Proteintech, China), p-AKT (1:1000, 66444-1-Ig, Proteintech, China), Mtor (1:1000, 66888-1-Ig, Proteintech, China), GAPDH (1:1000, 60004, Proteintech, China), and p-mTOR (1:1000, 67778-1-Ig, Proteintech, China) in shake asks at 4°C overnight. After the PVDF membranes were washed three times, the secondary antibody (goat anti-rabbit, 1:2000, SA00001-2, Proteintech, China and goat anti-mouse, 1:3000, SA00001-1, Proteintech, China) was incubated. Finally, we used enhanced chemiluminescent chromogenic substrates to observe the proteins.

Guo Y., Jiang L., Luo S., Hu D., Zhao X., Zhao G, & Tang W. (2024). Network Analysis and Basic Experiments on the Inhibition of Renal Cancer Proliferation and Migration by Alpinetin through PI3K/AKT/ mTOR Pathway. Current molecular medicine, 24(1).

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