Hydroxybupropion

Briefly, 1,25(OH)₂D₃, ACET, acetaminophen glucuronide (AG), BUP, hydroxybupropion (HBUP), carbamazepine (CARB), diltiazem (DIL), theophylline (THEO), TOL, 4-hydroxytolbutamide (HTOL), and uridine diphosphate glucuronic acid (UDPGA) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Dihydronicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Corning, Inc. (New York, NY, USA). All solvents used were of HPLC grade. The PrimeScriptTM 1st strand cDNA Synthesis Kit and SYBR^® Premix Ex TaqTM II ROX plus were purchased from Takara Bio, Inc. (Shiga, Japan). Forward and reverse primers for quantitative polymerase chain reaction (qPCR) experiment were synthesized by Bioneer Co. (Daejeon, Korea).

Phenacetin, bupropion, tolbutamide, dextromethorphan, midazolam, chlorzoxazone, and the diazepam (all purity> 98%) that used as internal standards (ISs) were purchased from J&K Scientific Ltd. (Beijing, China). Vonoprazan was purchased from Beijing Sunflower Scientific Ltd. (Beijing, China). Hydroxybupropion and hydroxymidazolam were purchased from Sigma-Aldrich (St Louis, USA). Dextrorphan, hydroxytolbutamide, hydroxychlorzoxazone, and 4-acetamidophenol were purchased from Toronto Research Chemicals (Toronto, Canada). Reduced nicotinamide adenine dinucleotide phosphate (NADPH) was acquired from Roche Pharmaceuticals Ltd. (Basel, Switzerland). Ultra-pure water was produced by Milli-Q, a reagent-standard water purification system (Millipore, Bedford, USA). Acetonitrile and methanol that of high performance liquid chromatography (HPLC) grade were obtained from Merck Company (Darmstadt, Germany).

Hon (purity ≥ 99%) was obtained from TCAS Chem Tech Co., Ltd. (Wuhan, China); Strepotozotocin, hydroxybupropion and 12-hydroxy Lauric acid were purchased from Sigma Chem Co., Ltd. (St. Louis, MO, USA). Bupropion and 6β-hydroxy testosterone were purchased from Cayman Chem Co., (Ann Arbor, MI, USA). Phenacetinwas obtained from Alfa Aesar Chem Co., Ltd. (Tianjin, China); Chlorzoxazone was purchased from National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Tolbutamide and testosterone were purchased from Dr. Ehrenstorfer GmbH, (Augsburg, Germany). Lauric acid was purchased from Nu-Chek Prep, Inc. (Los Angeles, CA, USA). Acetaminophen was purchased from TCI (Tokyo, Japan). 6-hydroxychlorzoxazoneand 4-hydroxyTolbutamidewere purchased from TRC (Toronto, ON, Canada). NADPH was purchased from Biosharp (Hefei, China). HPLC-grade methanol and acetonitrile were purchased from Tedia Co., Inc. (Fairfield, OH, USA). RT-PCR Kit and Trans Start Green qPCR Super Mix were purchased from Toyobo Co., Ltd. (Osaka, Japan). Real-time fluorescent quantitative PCR primers were purchased from Sunny Biotech. Co., Ltd. (Shanghai, China). All other chemicals and reagents used in the study were of analytical grade.