Plin 1

Manufactured by Progen Biotechnik

The PLIN 1 is a laboratory equipment designed for cellular analysis. It is a compact and automated instrument that measures the expression of the PLIN 1 protein, which is involved in lipid droplet formation and metabolism.

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Lab products found in correlation

Cocktail of protease and phosphatase inhibitors, by Merck Group (1 mentions) Plin5, by Progen Biotechnik (1 mentions) Gradient sds page gel, by Bio-Rad (1 mentions) Ripa buffer, by Merck Group (1 mentions) Image lab software, by Bio-Rad (1 mentions) Chemidoc mp system, by Bio-Rad (1 mentions) Glut1, by Abcam (1 mentions) Dcp00, by R&D Systems (1 mentions) β actin, by Merck Group (1 mentions) D6050, by R&D Systems (1 mentions)

2 protocols using plin 1

Protein Expression Analysis in Adipose Tissue

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Cells were lysed in RIPA buffer (Sigma) containing cocktail of protease and phosphatase inhibitors (Sigma). Equal amounts of solubilized proteins were loaded on 4–20% gradient SDS-PAGE gels (Bio-Rad), blotted onto nitrocellulose membranes and incubated with the following primary antibodies: HSL (#4107), ATGL (#2138) and GAPDH (#2118) were purchased from CST Ozyme; total PDHE1α (#Ab110334), phospho-Ser293 PDHE1α (#Ab177461), UCP1 (#Ab10983) and GLUT1 (#Ab40084) from Abcam; PLIN5 (#GP31) and PLIN 1 (#GP29) from PROGEN; PDK4 (#H00005166-A02) from Abnova and PCK1 (#AP8093b) from Abgent. Anti-rabbit or anti-mouse IgG coupled to horseradish peroxidase were used as secondary antibodies. Immunoreactive proteins were determined by chemiluminescence (Clarity, Bio-Rad) with a ChemiDoc MP System (Bio-Rad) and quantification was performed using Image Lab software (Bio-Rad).

Barquissau V., Beuzelin D., Pisani D.F., Beranger G.E., Mairal A., Montagner A., Roussel B., Tavernier G., Marques M.A., Moro C., Guillou H., Amri E.Z, & Langin D. (2016). White-to-brite conversion in human adipocytes promotes metabolic reprogramming towards fatty acid anabolic and catabolic pathways. Molecular Metabolism, 5(5), 352-365.

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Quantification of Inflammatory Markers

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Protein levels of human IL6, CCL2, TNFα (D6050, DCP00 and QTA00B, R&D Systems, Minneapolis, MN) and adiponectin (10-1193-01, Mercodia, Uppsala, Sweden) in conditioned media were measured using ELISA according to the manufacturer’s instructions. Western blot was performed as described previously (Mejhert et al., 2013 ). Primary antibodies were PLIN1 (Progen, Heidelberg, Germany), LIPE (Cell Signalling Technologies, Beverly, MA) and βactin (Sigma-Aldrich).

Gao H., Mejhert N., Fretz J.A., Arner E., Lorente-Cebrián S., Ehrlund A., Dahlman-Wright K., Gong X., Strömblad S., Douagi I., Laurencikiene J., Dahlman I., Daub C.O., Rydén M., Horowitz M.C, & Arner P. (2014). Early B-cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue. Cell metabolism, 19(6), 981-992.

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