Mouse serum amyloid a quantikine elisa kit

Serum amyloid A was measured in mouse serum samples using the Mouse Serum Amyloid A Quantikine ELISA kit (R&D Systems, Abingdon, UK) according to the manufacturer’s instructions.

Blood was collected from mice and transferred to BD Microtainer Serum Separator Tubes (Becton Dickinson, Franklin Lakes, NJ). Serum was flash frozen in liquid nitrogen and stored at -80C. Serum chemistry analysis for total cholesterol (CHOL), non-esterified fatty acids (NEFA), and triglycerides (TG) was performed using Wako Clinical Diagnostics kits (WakoUSA, Richmond, VA). Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured using Catachem VETSPEC Kits as recommended by the manufacturer (Catachem, Oxford, CT). Blood glucose (GLU) levels were measured using a Contour blood glucose meter (Bayer, Mishawaka, IN). Serum SAA1 analysis was performed using Mouse Serum Amyloid A Quantikine ELISA Kit (R&D Systems, Minneapolis, MN).

We collected mouse plasma using Ethylenediamine-N, N, N', N’-tetraacetic Acid Dipotassium Salt Dihydrate (2 K) (FUJIFILM Wako, CAS RN:25102-12-9). Mouse plasma SAA (Serum Amyloid A), Albumin, IL-6 (Interleukin 6), and GDF15 (Growth and Differentiation Factor 15) were quantified in duplicate using commercial ELISA kits (Mouse Serum Amyloid A Quantikine ELISA kit (R&D systems, Cat# MSAA00; lot number, not recorded; dilution, NA), Mouse Albumin ELISA kit (Abcam, Cat# ab207620; lot number, not recorded; dilution, NA), Mouse IL-6 ELISA kit (Merk, Cat#RAB0308; lot number, not recorded; dilution, NA), Mouse/Rat GDF-15 Quatikine ELISA kit (R&D systems, Cat#MGD150; lot number, not recorded; dilution, NA)). The absorbance of each well at 450 nm was determined with a Multiskan FC Microplate Photometer (Thermo Fisher Scientific, MA, USA; Catalog number: 51119000). For plasma IL-6 quantification, undetectable values were regarded as 0 pg/mL (n = 11 samples), and values below the minimum detectable dose (MDD) of IL-6 (2 pg/ml) were included without adjustment (n = 6 samples).