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Lbis porcine insulin elisa kit

Manufactured by Fujifilm
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The LBIS Porcine Insulin ELISA Kit is a laboratory equipment product designed for the quantitative determination of porcine insulin levels in biological samples using the enzyme-linked immunosorbent assay (ELISA) technique.

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Lab products found in correlation

Protease and phosphatase inhibitor cocktail, by Nacalai Tesque (2 mentions) Ripa buffer, by Nacalai Tesque (2 mentions) Glucagon elisa kit, by Fujifilm (2 mentions)

2 protocols using lbis porcine insulin elisa kit

1

Quantifying Insulin and Glucagon in Organoids

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Internal insulin and glucagon were extracted from 300 IEQs and organoids using 1 mL RIPA buffer (Cat#16488-34; Nacalai Tesque, Kyoto, Japan) containing × 100 protease and phosphatase inhibitor cocktails (Cat#07575-51 and Cat#07574-61; Nacalai Tesque). The insulin and glucagon contents were measured using an LBIS Porcine Insulin ELISA Kit and a Glucagon ELISA Kit (Wako), respectively.
Sakata N., Yoshimatsu G., Kawakami R., Nakano K., Yamada T., Yamamura A., Nagashima H, & Kodama S. (2024). The porcine islet-derived organoid showed the characteristics as pancreatic duct. Scientific Reports, 14, 6401.
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2

Insulin and Glucagon Quantification

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Insulin and glucagon were extracted from 300 IEQs using 1 mL RIPA buffer (Cat#16488-34; Nacalai Tesque, Kyoto, Japan) containing ×100 protease and phosphatase inhibitor cocktails (Cat#07575-51 and Cat#07574-61; Nacalai Tesque). The insulin and glucagon concentrations were measured using an LBIS Porcine Insulin ELISA Kit and a Glucagon ELISA Kit (Wako), respectively.
Sakata N., Yoshimatsu G., Kawakami R., Aoyagi C, & Kodama S. (2023). Optimal temperature for the long-term culture of adult porcine islets for xenotransplantation. Frontiers in Immunology, 14, 1280668.
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