The DR5 siRNA used in this study was purchased from Invitrogen (Calsbad, CA, USA). Mcl-1 siRNA and sphingosine kinase 1 siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The siRNA had the following sequences: DR5, AUC AGC AUC GUG UAC AAG GUG UCC C; and green fluorescent protein [GFP (control)], AAG ACC CGC GCC GAG GUG AAG. Cells were transfected with siRNA oligonucleotides using Oligofectamine reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's recommendations.
Dr5 sirna
Manufactured by Thermo Fisher Scientific
Sourced in United States
The DR5 siRNA is a laboratory reagent designed for RNA interference (RNAi) experiments. It is a double-stranded RNA molecule that targets the DR5 (Death Receptor 5) gene, which is involved in the apoptosis (programmed cell death) pathway. The DR5 siRNA can be used to specifically silence the expression of the DR5 gene in research studies, allowing researchers to investigate its role in cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti mcl 1, by Santa Cruz Biotechnology (2 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (2 mentions)
Z vad fmk, by R&D Systems (2 mentions)
Anti actin, by Merck Group (2 mentions)
Gfp control sirna, by Bioneer (2 mentions)
Anti bim, by BD (2 mentions)
Anti bcl 2, by Santa Cruz Biotechnology (2 mentions)
Anti dr4, by Abcam (2 mentions)
Anti ciap2, by Santa Cruz Biotechnology (2 mentions)
Anti c flip, by Enzo Life Sciences (2 mentions)
Anti dr5, by Cell Signaling Technology (2 mentions)
Anti survivin, by R&D Systems (2 mentions)
Anti bcl xl, by Cell Signaling Technology (2 mentions)
Anti xiap, by BD (2 mentions)
Anti parp, by Cell Signaling Technology (2 mentions)
Mcl 1 sirna, by Santa Cruz Biotechnology (1 mentions)
Oligofectamine reagent, by Thermo Fisher Scientific (1 mentions)
Cisplatin, by R&D Systems (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Oxaliplatin, by Merck Group (1 mentions)
4 protocols using dr5 sirna
1
Targeted Gene Silencing using siRNA
2
Molecular Mechanisms of TRAIL-Induced Apoptosis in Cancer Cells
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Selleckchem supplied R428 and cisplatin (Houston, TX, USA), and R&D system supplied recombinant human recombinant TRAIL and z-VAD-fmk (Minneapolis, MN, USA). Sigma Chemical Co. provided MG132, cycloheximide, carboplatin, oxaliplatin, doxorubicin, and 5-FU (St. Louis, MO, USA). The primary antibodies were as follows: Anti-phospho-Axl (Y702) (Cell Signaling Technology, Beverly, MA, USA), anti-pro-caspase-3 (Cell Signaling Technology), anti-cleaved caspase-3 (Cell Signaling Technology), anti-PARP (Cell Signaling Technology), anti-Bcl-xL (Cell Signaling Technology), anti-DR5 (Cell Signaling Technology), anti-actin (Sigma Chemical Co.), anti-Axl (Santa Cruz Biotechnology, St. Louis, MO, USA), anti-Mcl-1 (Santa Cruz Biotechnology), anti-Bcl-2 (Santa Cruz Biotechnology), anti-cIAP2 (Santa Cruz Biotechnology), anti-Bim (BD Biosciences, San Jose, CA, USA), anti-XIAP (BD Biosciences), anti-survivin (R&D system, Minneapolis, MN, USA), anti-DR4 (Abcam, Cambridge, MA, USA), and anti-c-FLIP (Enzo Life Sciences, San Diego, CA, USA). The siRNAs were as follows: GFP (control) siRNA (Bioneer, Daejeon, Korea), Axl siRNA (Santa Cruz Biotechnology), and DR5 siRNA (Invitrogen).
3
Stable Cell Line Generation and Knockdown
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For developing stable cell lines, Caki-1 cells were transfected with the control plasmid pcDNA 3.1(+) vector or pcDNA 3.1(+)/survivin-flag plasmids using Lipofectamine™2000 (Invitrogen, Carlsbad, CA, USA). After 24 h, cells were picked by 700 μg/mL G418 (Invitrogen, Carlsbad, CA, USA). For knockdown of gene, Caki-1 cells were transfected with the control siRNA (Bioneer, Daejeon, Korea), DR5 siRNA (Invitrogen, Carlsbad, CA, USA) or Cbl siRNA (Santa Cruz Biotechnology, St. Louis, MO, USA) using Lipofectamine® RNAiMAX Reagent (Invitrogen, Carlsbad, CA, USA). Furthermore, protein expressions were checked by western blotting.
4
Dissecting TRAIL-induced Apoptosis Signaling
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Sigma Chemical Co. provided honokiol, cycloheximide and MG132 (St. Louis, MO, USA), and R&D system supplied recombinant human recombinant TRAIL and z-VAD-fmk (Minneapolis, MN, USA). Enzo Life Sciences provided lactacystin (Ann Arbor, MI, USA). The primary antibodies were as follows: Cell Signaling Technology supplied anti-PARP, anti-cleaved caspase-3, anti-Bcl-xL, anti-DR5, anti-CHOP, and anti-UCHL1 (Beverly, MA, USA). Sigma Chemical Co. supplied anti-actin (St. Louis, MO, USA). Enzo Life Sciences provided anti-pro-caspase-3 and anti-c-FLIP (San Diego, CA, USA). BD Biosciences provided anti-Bim and anti-XIAP (San Jose, CA, USA). Abcam supplied anti-DR4 (Cambridge, MA, USA). R&D system supplied anti-survivin (Minneapolis, MN, USA). Santa Cruz Biotechnology provided anti-Mcl-1, anti-Bcl-2, anti-cIAP2, anti-ATF4, anti-Ub, anti-Cbl, anti-Itch, anti-USP14, anti-USP33, anti-OTUB1, anti-TRABID, and anti-STAMBPL1 (St. Louis, MO, USA). Bethyl Laboratories Inc provided anti-USP7 and anti-USP8 (Montgomery, TX, USA). Novus Biologicals supplied anti-USP53 (Centennial, CO, USA). Abnova provided anti-USP9X (Taipei City, Taiwan). The siRNAs were as follows: GFP (control) siRNA (Bioneer, Daejeon, Korea), DR5 siRNA (Invitrogen, Carlsbad, CA, USA), and STAMBPL1 siRNA (Santa Cruz Biotechnology, St. Louis, MO, USA). STAMBPL1 plasmid was a gift from Dr. H.C. Kang (Ajou University, Suwon, Korea).
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