Western Blot analyses were performed using rabbit anti-Kpnβ1 (H300) (Santa Cruz Biotechnology, sc-11367), mouse anti-GAPDH (0411) (Santa Cruz Biotechnology, sc-47724), rabbit anti-GFP (FL) (Santa Cruz Biotechnology, sc-8334), rabbit anti-Cyclin A (H-432) (sc-751), rabbit anti-Cyclin D1 (HD11) (sc-246), rabbit anti-pHisH3 (Ser-10)-R (Santa Cruz Biotechnology, sc-8656), rabbit anti-Mcl-1 (H-260) (Santa Cruz Biotechnology, sc-20679), rabbit anti-E-cadherin (H-108) (Santa Cruz Biotechnology, sc-7870), mouse anti-Vimentin (V-9) (Santa Cruz Biotechnology, sc-6260), rabbit anti-PARP1/2 (H-250) (Santa Cruz Biotechnology, sc-7150), mouse anti-p53 (DakoCytomation, M7001), rabbit anti-p21 (H-164) (Santa Cruz Biotechnology, sc-756), rabbit anti-γH2AX (Ser-139) (Cell Signaling, 2577S), and rabbit anti-TFIID (TBP) (N-12) (Santa Cruz Biotechnology, sc-204) antibodies.
Mouse anti vimentin v 9
Manufactured by Santa Cruz Biotechnology
Mouse anti-Vimentin (V-9) is a monoclonal antibody that recognizes the vimentin protein. Vimentin is a type III intermediate filament protein found in various non-epithelial cells. This antibody can be used for the detection and localization of vimentin in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti p21 h 164, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti mcl 1 h 260, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti cyclin a h 432, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti e cadherin h 108, by Santa Cruz Biotechnology (1 mentions)
Mouse anti gapdh 0411, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti gfp fl, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti γ h2ax ser139, by Cell Signaling Technology (1 mentions)
Alexa fluor fluorophores, by Thermo Fisher Scientific (1 mentions)
Scan r imaging platform, by Olympus (1 mentions)
Delta vision imaging system, by Cytiva (1 mentions)
Dapi fluoromount g, by Southern Biotech (1 mentions)
2 protocols using mouse anti vimentin v 9
1
Comprehensive Western Blot Analysis
2
Immunofluorescence Analysis of Cellular Proteins
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HeLa cells grown on coverslips were fixed using 4% PFA in PBS. Permeabilization was performed using 0.2% Triton X‐100 in PBS for 10 min. 3% BSA in PBS was used for blocking. All antibodies were diluted in 1% BSA in PBS. The following antibodies were used: goat anti‐eIF3η (N‐20, Santa Cruz Biotechnology), mouse anti‐polyubiquitin (FK1, Enzo Life Sciences), mouse anti‐HSP27 (ADI‐SPA‐800, Enzo Life Sciences), mouse anti‐HSP70 (ADI‐SPA‐810, Enzo Life Sciences), mouse anti‐VCP (MA3‐004, Thermo Fisher Scientific), mouse anti‐p62/SQSTM1 (D‐3, Santa Cruz Biotechnology), mouse anti‐TARDBP (41‐7.1, Santa Cruz Biotechnology), and mouse anti‐vimentin (V9, Santa Cruz Biotechnology). Secondary antibodies conjugated with Alexa Fluor fluorophores (Invitrogen) were then applied, followed by washing in PBS. Coverslips were mounted on microscope slides in DAPI‐Fluoromount G (SouthernBiotech). The cells were then imaged using the DeltaVision imaging system (Applied Precision) or the Scan^R imaging platform (Olympus), as described above.
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