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Gm7 microstat

Manufactured by Analox Instruments
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Sourced in United Kingdom

The GM7 MicroStat is a compact, self-contained gas monitoring device designed for accurate measurement of gas concentrations. It features an electrochemical sensor technology to detect and display the current gas levels. The GM7 MicroStat provides a straightforward interface for monitoring and reporting gas data.

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Lab products found in correlation

Reflotron plus, by Roche (1 mentions) Humulin r, by Eli Lilly (1 mentions) Gmrd 103, by Analox Instruments (1 mentions) 9618s 10d micro touph electrode, by Horiba (1 mentions) Laqua f 72, by Horiba (1 mentions)

Close spelling variants of this product

Analox analyzer model GM7 MicroStat Analox analyzer GM7 MicroStat

5 protocols using gm7 microstat

1

Quantification of Brain Lactate and Glucose

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The concentration of lactate in the brain homogenates was determined using a multi-assay analyzer (GM7 MicroStat; Analox Instruments, London, UK) in accordance with the manufacturer’s instructions. In our prior tests using several samples, we loaded 5, 10, and 20 μl of supernatant to the instrument, observing linear, volume-dependent increases in the measured values (r2>0.99). Based on these results, we used 20 μl of supernatant for lactate measurements. Similarly, glucose concentrations in 20 μl supernatant samples were determined using a multiassay analyzer following calibration with 10 mmol/ml glucose standard solution. To normalize the effects of differences in genetic background and age among strains, Z-scores for pH and lactate levels were calculated within each strain and used for the correlation analysis.
Hagihara H., Catts V.S., Katayama Y., Shoji H., Takagi T., Huang F.L., Nakao A., Mori Y., Huang K.P., Ishii S., Graef I.A., Nakayama K.I., Shannon Weickert C, & Miyakawa T. (2017). Decreased Brain pH as a Shared Endophenotype of Psychiatric Disorders. Neuropsychopharmacology, 43(3), 459-468.
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2

3-MPA Physiological Effects in Fasted Rodent

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The physiological effects of 3-MPA were tested in a bench experiment with an overnight-fasted animal (450 g). The inhibitor injection protocol was identical as mentioned above. Respiration and temperature of the animal were monitored continuously. 100 μl-blood samples were collected from the tail vein before the injection and then every 30 min over 2.5 h. Blood glucose and plasma lactate levels were measured using automated glucose/lactate analyzers (Reflotron Plus, Roche, Basel, Switzerland and GM7 Micro-Stat, Analox Instruments, London, UK).
Can E., Bastiaansen J.A., Couturier D.L., Gruetter R., Yoshihara H.A, & Comment A. (2022). [13C]bicarbonate labelled from hyperpolarized [1-13C]pyruvate is an in vivo marker of hepatic gluconeogenesis in fasted state. Communications Biology, 5, 10.
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3

Comprehensive Metabolic Assessment in Pank1 Mice

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Blood glucose was measured using a glucometer (FreeStyle, Abbott Diabetes Care, Alameda, CA, USA); insulin was measured using an ELISA kit (Crystal Chem, Downers Grove, IL, USA); lactate and β-hydroxybutyrate were determined using a GM7 Micro-Stat analyser (Analox Instruments, London, UK); free fatty acids were determined using a Biovision (Milipitas, CA, USA) assay kit; triglycerides and cholesterol were analysed by the St. Jude Veterinary Pathology Core, amino acids were measured by the University of California at Davis Proteomic Core. Prior to OGTT with 2 g/Kg glucose, Pank1+/+ and Pank1−/− mice were fasted overnight. Prior to OGTT with 0.25 g/Kg glucose Pank1+/+Lep−/− and Pank1−/−Lep−/− mice were fasted 24h. For insulin tolerance tests (ITT) with 0.75 U/Kg insulin (Humulin R, Eli Lilly, Indianapolis, IN, USA) i.p., Pank1+/+ and Pank1−/− mice were fasted overnight. For ITT with 7.5 U/Kg, Pank1+/+Lep−/− and Pank1−/−Lep−/− mice were fasted 6h. For alanine tolerance tests with 0.25g/Kg i.p., Pank1+/+Lep−/− and Pank1−/−Lep−/− mice were fasted 24h. AUC for individual mice were reported as the mean ± SEM and was calculated using initial blood glucose as baseline. For ITT, individual glucose curves were normalized to initial blood glucose set to 100 and AUC calculated using y=0 as baseline.
Leonardi R., Rock C.O, & Jackowski S. (2014). Pank1 Deletion in Leptin-deficient Mice Reduces Hyperglycaemia and Hyperinsulinemia and Modifies Global Metabolism without Affecting Insulin Resistance. Diabetologia, 57(7), 1466-1475.
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4

Quantifying Brain pH and Lactate

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Whole brain was used to measure pH and lactate levels as previously described (29) .
Briefly, snap-frozen tissues were homogenized in ice-cold distilled H2O (5 ml per 500 mg of tissue). The pH of the homogenates was measured using a pH meter (LAQUA F-72, HORIBA, Ltd., Kyoto, Japan) equipped with a Micro ToupH electrode (9618S-10D, HORIBA, Ltd.) after a three-point calibration at pH 4.0, pH 7.0, and pH 9.0. Subsequently, the concentration of lactate in the homogenates was determined using a multi-assay analyzer (GM7 MicroStat, Analox Instruments, London, UK) after calibration with 8.0 M lactate standard solution (GMRD-103, Analox Instruments). A 20-µl aliquot of centrifuged supernatant (14,000 rpm, 10 min) was used for the measurement.
Effect size (d) was calculated for each strain/condition and each measure (e.g., pH, lactate value, and behavioral index), as followed:
The heat map was depicted using the R (version 3.5.2) gplots package.
Z-score transformation, a traditional method of data normalization for direct comparison between different samples and conditions, was applied for each pH or lactate value using individual animal data within each of strain, according to the following formula:
Z-score = (valueP -mean valueP1…Pn)/standard deviationP1…Pn , where P is any pH or lactate and P1…Pn represent the aggregate measure of all pH or lactate values.
Hagihara H., Shoji H., Hattori S., Sala G., Takamiya Y., Tanaka M., Ihara M., Shibutani M., Hatada I., Hori K., Hoshino M., Nakao A., Mori Y., Okabe S., Matsushita M., Urbach A., Katayama Y., Matsumoto A., Nakayama K.I., Katori S., Sato T., Iwasato T., Nakamura H., Goshima Y., Raveau M., Tatsukawa T., Yamakawa K., Kasai H., Takahashi N., Inazawa J., Nobuhisa I., Kagawa T., Taga T., Darwish M., Nishizono H., Takao K., Sapkota K., Nakazawa K., Takagi T., Fujisawa H., Sugimura Y., Yamanishi K., Rajagopal L., Hannah N.D., Meltzer H.Y., Yamamoto T., Wakatsuki S., Araki T., Tabuchi K., Numakawa T., Kunugi H., Huang F.L., Hayata‐Takano A., Hashimoto H., Tamada K., Takumi T., Kasahara T., Kato T., Graef I.A., Crabtree G.R., Asaoka N., Hatakama H., Kaneko S., Kohno T., Hattori M., Hoshiba Y., Miyake R., Obi-Nagata K., Hayashi‐Takagi A., Becker L.J., Yalçın İ., Hagino Y., Kotajima‐Murakami H., Moriya Y., Ikeda K., Kim H., Kaang B., Otabi H., Yoshida Y., Toyoda A., Komiyama N.H., Grant S.G.N., Ida‐Eto M., Narita M., Matsumoto K., Okuda‐Ashitaka E., Ohmori I., Shimada T., Yamagata K., Ageta H., Tsuchida K., Inokuchi K., Sassa T., Kihara A., Fukasawa M., Usuda N., Katano T., Tanaka T., Yoshihara Y., & Igarashi M. (2021). Large-scale animal model study uncovers altered brain pH and lactate levels as a transdiagnostic endophenotype of neuropsychiatric disorders involving cognitive impairment.
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5

Serum Biomarker Quantification

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Serum adiponectin, insulin and leptin were determined by ELISA (R&D Systems; Alpco, Salem, NH, USA and Millipore respectively). All ELISAs were performed according to the manufacturer's instructions using the online data analysis tool MyAssays Ltd. (http://www.myassays.com; 5th December 2014, East Sussex, UK). Serum glucose was analysed using the GM7 MicroStat (Analox instruments, Stourbridge, UK). Serum levels of non-esterified fatty acid (NEFA), triglycerides and glycerol were measured using commercially available assays (Wako; Sigma-Aldrich).
Russell P.K., Mangiafico S., Fam B.C., Clarke M.V., Marin E.S., Andrikopoulos S., Wiren K.M., Zajac J.D, & Davey R.A. (2018). The androgen receptor in bone marrow progenitor cells negatively regulates fat mass. The Journal of endocrinology, 237(1).
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