Recombinant Human TPP1 protein (lot #BIQE03) was a generous gift from BioMarin Pharmaceutical Inc. (Novato, CA, USA). GenePORTER 3000 transfection agent was purchased from AMS Biotechnology (Milton, Abingdon, UK). Lipophilic fluorescent dyes, 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine Perchlorate (DID) and 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) were purchased from Invitrogen (Carlsbad, CA, USA) and Sigma-Aldrich (St. Louis, MO, USA), respectively. FITC-conjugated mouse antibodies to LAMP1 were purchased from BD Biosciences (San Diego, CA, USA). Cell culture medium and fetal bovine serum (FBS) were purchased from Gibco Life Technologies, (Grand Island, NY, USA). Human TPP1/CLN2 ELISA Kit was obtained from LifeSpan BioSciences, Inc. (Seattle, WA, USA). TPP1-encoding pDNA (pSJG-JaTl-hTPP1opt-myc-spa) was a generous gift from Dr. Steven J. Gray (Department of Ophthalmology Gene Therapy Center, UNC). TPP1 substrate, AF-AMC and Triton X-100 were obtained from Sigma-Aldrich. Neuronal growth factor (NGF) was obtained from Gemini Bio-Products (West Sacramento, CA, USA).
Geneporter 3000 transfection agent
Manufactured by AMS Biotechnology
Sourced in United Kingdom
The GenePORTER 3000 is a transfection agent designed for the efficient delivery of nucleic acids, such as DNA and RNA, into cells. It facilitates the transport of these genetic materials across the cell membrane, enabling researchers to study gene expression and function.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (2 mentions)
4 6 diamidino 2 phenylindole dihydrochloride dapi, by Thermo Fisher Scientific (1 mentions)
Cell culture medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine perchlorate did, by Thermo Fisher Scientific (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dil, by Thermo Fisher Scientific (1 mentions)
Murine macrophage colony stimulating factor m csf, by Thermo Fisher Scientific (1 mentions)
Interferon gamma int γ, by Thermo Fisher Scientific (1 mentions)
2 protocols using geneporter 3000 transfection agent
1
Recombinant TPP1 Protein Characterization
2
Efficient Transfection and Cellular Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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GenePORTER 3000 transfection agent was purchased from AMS Biotechnology, England). Lipopolysaccharides (LPS), 6-hydroxydopamine (6-OHDA), and Triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO, USA). A lipophilic fluorescent dye, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindo-carbocyanine perchlorate (DIL), was purchased from Invitrogen (Carlsbad, CA). Interferon gamma (INT-γ), and murine macrophage colony-stimulating factor (MCSF) were purchased from Peprotech Inc (Rocky Hill, NJ).
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