Prothrombin time, aPTT, and fibrinogen were measured using standard operating procedures on an ACL-300 or ACL-ELITE automated coagulation analyzer (Instrumentation Laboratory, Bedford, Massachusetts). This instrument uses an optical method to detect clot formation in a plasma sample. For aPTT, Platelin (Diagnostica Stago, Parsippany, New Jersey) was used along with 0.025 M CaCl2 to recalcify the citrated plasma. For PT/INR and fibrinogen, Recombiplastin (Instrumentation Laboratory) was used.
Acl elite
Manufactured by Werfen
Sourced in United States
The ACL ELITE is a coagulation analyzer designed for automated blood coagulation testing. It performs various tests, including prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen quantification. The ACL ELITE provides accurate and reliable results to support clinical decision-making in the diagnosis and monitoring of coagulation disorders.
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Lab products found in correlation
Human thrombin, by Enzyme Research (9 mentions)
Bovine xa, by Enzyme Research (6 mentions)
Triniclot aptt reagent, by Diagnostica Stago (4 mentions)
Recombiplastin, by Werfen (3 mentions)
Spectrozyme xa, by Sekisui (2 mentions)
Acl 300, by Werfen (1 mentions)
Sta compact, by Diagnostica Stago (1 mentions)
Coag dx, by IDEXX (1 mentions)
Advia 2120, by Siemens (1 mentions)
Element ht5, by Heska (1 mentions)
Hemosil synthasil kit, by Werfen (1 mentions)
St4 instrument, by Diagnostica Stago (1 mentions)
Immulite immulite 1000 cortisol, by Siemens (1 mentions)
Vacutainer, by BD (1 mentions)
Cobas integra 400 plus, by Roche (1 mentions)
Vitros 350 chemistry system, by Ortho Clinical Diagnostics (1 mentions)
Spectramax m5, by Molecular Devices (1 mentions)
19 protocols using acl elite
1
Coagulation Panel Measurements: ACL Analyzer
2
Comparative Analysis of Hematological and Coagulation Profiles
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Complete blood counts were performed using automated analyzers (ADVIA 2120, Siemens Healthcare Diagnostics, Tarrytown, New York, Element HT5, Heska Corp. Loveland, Colorado). No adjustments were made to these counts to account for minor differences in RIs among centers. Coagulation times were measured using whole blood (CoagDx, IDEXX, Westbrook, ME; VS Pro, Abaxis Inc, Union City, California) or plasma (STA Compact, Diagnostica Stago, Parsippany, New Jersey; ACL Elite, Instrumentation Laboratory, Bedford, Massachusetts). The RIs for these methods were different, precluding comparisons of these results as continuous variables. Thus, results from each center were recorded as low (below RI), normal (within RI), or high (above RI) to enable comparisons.
3
Coagulation Assays of Micelle-FFP Mixture
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60μL of micelle solution was mixed with 540μL of FFP and then incubated at 37 ºC for 1h, after which the mixture was centrifuged at 2500 rpm for 10 minutes at 4 ºC to collect supernatant. Four coagulation assays were carried out, eg, APTT, PT, TT, and Fib, using an automated coagulation analyzer (Instrumentation Laboratory ACL ELITE, USA). Heparin (HP, 0.75 IU/mL) was used as a positive control, while NS was used as a normal control.
4
Coagulation Marker Assessment Protocol
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Prothrombin time/Inetrnational Normalized Ratio (PT/INR) and fibrinogen, required for computation of the DIC score, were measured using standard operating procedures on an ACL-ELITE automated coagulation analyzer (Instrumentation Laboratory, Bedford, Massachusetts). This instrument uses an optical method to detect clot formation in a plasma sample. Recombiplastin (Instrumentation Laboratory) was used as the PT reagent.
5
Evaluating Factor Xa Inhibitor Impact
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Factor Xa inhibitors were supplemented in citrated plasma in a concentration range of 1.0 to 0.062 μg/mL. The PT, aPTT, and Heptest (Haemachem, St. Louis, Missouri) were measured using an ACL-Elite (Instrumentation Laboratory, Bedford, Massachusetts). The PiCT was measured on the ST4. Results were compiled in terms of mean (SD; n = 5).
6
Heparin Potency Determination in Plasma
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Ovine heparin and enoxaparin were supplemented in pooled normal human plasma and tested
in a concentration range of 0 to 10 μg/mL to determine potency. The amidolytic anti-IIa
and anti-Xa assays were performed on an ACL ELITE (Instrumentation Laboratory, Lexington,
Massachusetts) using bovine Xa and human thrombin (TH) from Enzyme Research Laboratories
(South Bend, Indiana). Chromogenic substrates, Spectrozyme Xa, and TH were obtained from
American Diagnostica (Stamford, Connecticut) or using kits from Aniara Diagnostica (West
Chester, Ohio). The potency of the ovine heparin was calculated using the USP standard for
heparin (lot FOI 187). The potency of each heparin was calculated based on the calibration
curve prepared with the USP heparin standard. The potency of the enoxaparin was calculated
using the National Institute for Biological Standards and Control (01/608; Potters Bar,
England) standard.
in a concentration range of 0 to 10 μg/mL to determine potency. The amidolytic anti-IIa
and anti-Xa assays were performed on an ACL ELITE (Instrumentation Laboratory, Lexington,
Massachusetts) using bovine Xa and human thrombin (TH) from Enzyme Research Laboratories
(South Bend, Indiana). Chromogenic substrates, Spectrozyme Xa, and TH were obtained from
American Diagnostica (Stamford, Connecticut) or using kits from Aniara Diagnostica (West
Chester, Ohio). The potency of the ovine heparin was calculated using the USP standard for
heparin (lot FOI 187). The potency of each heparin was calculated based on the calibration
curve prepared with the USP heparin standard. The potency of the enoxaparin was calculated
using the National Institute for Biological Standards and Control (01/608; Potters Bar,
England) standard.
7
Anti-FXa Activity Measurement in Rabbit Plasma
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Anti-FXa activity was measured by a modified assay using reagents from a commercial Heparin kit (Coamatic, DiaPharma), as described above for human plasma, substituting pooled rabbit plasma and using edoxaban as the standard. The anti-FXa activity in the plasma samples was calculated from the edoxaban standard curve and is expressed as ng/mL. Determination of PT and aPTT was performed on an Instrumentation Laboratory ACL Elite; PT using a HemosIL PT-Fibrinogen kit and aPTT using a HemosIL SynthASil kit (both kits from Instrumentation Laboratory).
8
Evaluating Coagulation Impact of Chitosan Oligosaccharide
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Activated partial thrombin time (APTT), prothrombin time (PT), thrombin time (TT) and the concentration of fibrinogen (Fib) were measured to determine the effect of COS on coagulation system by a modified strategy according to previous study (Nikitina et al., 2018 (link)). Briefly, COS stock solution (60 μl) were mixed with FFP (540 μl) to attain a final concentration of 0.01, 0.1, and 0.5 mg/ml and then was incubated at 37°C for 3 min. A coagulation analyzer (Instrumentation Laboratory ACL ELITE, USA) was used to test the incubated FFP. The parameter range available for the machine is 6 s to 245 s for APTT, 5 s to 165 s for PT, and 3 s to 169 s for TT. NS was used as standard control and heparin (HP, final concentration of 0.75 IU/ml) was used as positive control. Values of above coagulation tests were mean from three measurements and results were expressed as mean ± standard deviation (SD).
9
Automated Coagulation Analysis Protocol
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Prothrombin time (PT)/international normalized ratio (INR) and fibrinogen were
measured using standard operating procedures on an ACL-ELITE automated
coagulation analyzer (Instrumentation Laboratory, Bedford, Massachusetts). This
instrument uses an optical method to detect clot formation in a plasma sample.
Recombiplastin (Instrumentation Laboratory) was used as the PT reagent.
measured using standard operating procedures on an ACL-ELITE automated
coagulation analyzer (Instrumentation Laboratory, Bedford, Massachusetts). This
instrument uses an optical method to detect clot formation in a plasma sample.
Recombiplastin (Instrumentation Laboratory) was used as the PT reagent.
10
Anticoagulant Reversal Assay Validation
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Unfractionated heparin, enoxaparin, and fondaparinux were supplemented into citrated plasma over a concentration range of 0.62 to 10.0 µg/mL. Either saline as a control or andexanet alfa at a final concentration of 100 µg/mL was added to individual aliquots of plasma supplemented of each anticoagulant. Samples were analyzed using the TriniCLOT aPTT reagent (Diagnostica Stago, Parsippany, New Jersey). Human thrombin (Enzyme Research Laboratories, South Bend, Indiana) reconstituted with 0.025 M CaCl2 at a concentration of 5 U/mL was used to measure thrombin time (TT). All reagents were reconstituted according to the manufacturer’s instructions. Both aPTT and TT were measured using an ACL-Elite (Instrumentation Laboratory, Bedford, Massachusetts). Results were compiled as mean ± SD.
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