Deferoxamine, ferrostatin-1, erastin, sulfacetamide, sulfasalazine (SSZ), zymosan, diphenyleneiodonium chloride (DPI), 4,4′-diaminodiphenyl sulfone (DDS), sulfisoxazole, tBHP, and sulfasalazine were purchased from Sigma. Phorbol 12-myristate 13-acetate (PMA), sulfamethoxazole, sulfanilamide, sulfapyridine, 5-aminosalicylic acid (5-ASA), sulfadimidine, sulfametoxydiazine, sulfisomidine, sulfanitran, sulfanilamide, and piroxicam were purchased from Wako. We purchased 1-O-hexadecyl-2-arachidonoyl-sn-glycero-3-phosphocholine (ether-linked phosphatidylcholine) from Avanti polar lipids. Necrostatin-1 was purchased from FOCUS Biomolecules, and 2-mercaptoethanol was purchased from MP Biomedicals. z-VAD-fmk was purchased from Peptide institute. Trolox, sulfadiazine, sulfadimethoxine, sulfadoxin, and sulfamethoxypyridazine were purchased from Tokyo Chemical Industry. Cl-amidine was purchased from Cayman chemicals. Ionomycin was purchased from Merck Millipore.
Sulfanilamide
Manufactured by Fujifilm
Sourced in Japan
Sulfanilamide is a chemical compound used as a laboratory reagent. It is a white crystalline powder that is soluble in water and alcohol. Sulfanilamide is commonly used in various analytical and research applications in the laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Piroxicam, by Fujifilm (2 mentions)
Phosphoric acid, by Fujifilm (2 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (2 mentions)
Ionomycin, by Merck Group (1 mentions)
Zymosan, by Merck Group (1 mentions)
Ferrostatin 1, by Merck Group (1 mentions)
Deferoxamine, by Merck Group (1 mentions)
Erastin, by Merck Group (1 mentions)
Sulfasalazine, by Merck Group (1 mentions)
Sulfamethoxazole, by Fujifilm (1 mentions)
Sulfisoxazole, by Merck Group (1 mentions)
Sulfacetamide, by Merck Group (1 mentions)
Z vad fmk, by Peptide Institute (1 mentions)
Cl amidine, by Cayman Chemical (1 mentions)
Trolox, by Tokyo Chemical Industry (1 mentions)
Phorbol 12 myristate 13 acetate, by Fujifilm (1 mentions)
4 4 diaminodiphenylsulfone, by Merck Group (1 mentions)
2 mercaptoethanol, by MP Biomedicals (1 mentions)
Sodium nitrite, by Fujifilm (1 mentions)
N 1 naphthyl ethylenediamine, by Fujifilm (1 mentions)
7 protocols using sulfanilamide
1
Reagents for Cell Death Assays
2
Quantification of Inflammatory Mediators
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Splenocytes harvested from each group of mice were cultured for 48 h followed by collection of the supernatants. Levels of IFN-γ, TNF-α and IL-10 were determined with a corresponding ELISA kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions. To determine NO production, concentrations of NO2− in cell supernatants were measured by the Griess reaction [18 (link)]. Briefly, 100 μl of the supernatant was incubated with 100 μl of Griess reagent [equal volumes of 1 % (w/v) sulfanilamide (Wako, Osaka, Japan) and 0.1 % (w/v) N-1-naphtyl ethylenediamine dihydrochloride (Wako) in 2.5 % (w/v) H3PO4] for 10 min at room temperature, and NO2− concentration was determined by measuring the optical density at 550 nm (A550) in reference to the A550 of standard NaNO2 solution.
3
Lipopolysaccharide Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lipopolysaccharide (LPS), N-(1-naphthyl) ethylenediamine, sodium nitrite, and sulfanilamide were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). All other chemicals and reagents used in the study were of analytical grade or higher.
4
Curcuma longa Extracts Inhibit LPS-Induced Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used the following reagents: Dulbecco’s modified Eagle’s medium (DMEM), penicillin–streptomycin, LPS (Escherichia coli O127:B8; L3129), and dimethyl sulfoxide (Sigma-Aldrich, Saint Louis, MO, USA); fetal bovine serum (FBS) (HyClone, Logan, UT, USA); turmeronols A and B and bisacurone isolated from C. longa (Nagara Science, Gifu, Japan); and sulfanilamide, N-1-naphthylenediamide dihydrochloride, phosphoric acid, and sodium nitrite (Fujifilm Wako, Osaka, Japan).
5
Okinawan Vegetable Extracts Modulate Inflammatory Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RAW264 cells were treated with
1 μg/mL of Okinawan vegetable methanol extracts for 24 h. After
incubation, the supernatant was collected and used for NO and cytokine
assays. In the case of the NO assay, the supernatants were mixed with
Griess reagent containing equal volumes of 1% sulfanilamide (Wako
Pure Chemical Industry, Ltd., Osaka, Japan) in 2.5% phosphoric acid
and 0.1% naphthylethylenediamine dihydrochloride (Wako Pure Chemical
Industry, Ltd., Osaka, Japan) solution and then incubated at room
temperature for 10 min. The concentration of nitrite oxide was measured
by OD at 595 nm. NaNO2 (Wako Pure Chemical Industry, Ltd.,
Osaka, Japan) was used as a standard regent, while the determination
of cytokine concentrations was measured using a Quantikine enzyme-linked
immunosorbent assay kit (R&D Systems, Inc., Minneapolis, MN) for
IL-6 and TNF-α following the manufacturer’s instructions.
1 μg/mL of Okinawan vegetable methanol extracts for 24 h. After
incubation, the supernatant was collected and used for NO and cytokine
assays. In the case of the NO assay, the supernatants were mixed with
Griess reagent containing equal volumes of 1% sulfanilamide (Wako
Pure Chemical Industry, Ltd., Osaka, Japan) in 2.5% phosphoric acid
and 0.1% naphthylethylenediamine dihydrochloride (Wako Pure Chemical
Industry, Ltd., Osaka, Japan) solution and then incubated at room
temperature for 10 min. The concentration of nitrite oxide was measured
by OD at 595 nm. NaNO2 (Wako Pure Chemical Industry, Ltd.,
Osaka, Japan) was used as a standard regent, while the determination
of cytokine concentrations was measured using a Quantikine enzyme-linked
immunosorbent assay kit (R&D Systems, Inc., Minneapolis, MN) for
IL-6 and TNF-α following the manufacturer’s instructions.
6
Reagents for Cell Culture and Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isogen II was purchased from Nippon Gene Co., Ltd. (Tokyo, Japan). The FastGene Scriptase II cDNA Synthesis kit was purchased from NIPPON Genetics Co., Ltd. (Tokyo, Japan). DMSO, Dulbecco’s modified Eagle’s medium (DMEM; high glucose) without L-glutamine and phenol red, L-glutamine, LPS from E. coli O111, MEM nonessential amino acids (NEAA), Eagle’s minimum essential medium (EMEM) with L-glutamine and phenol red, N-1-Naphthylethylenediamine dihydrochloride, penicillin-streptomycin solution, phosphoric acid, and sulfanilamide were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Fetal bovine serum (FBS) was purchased from Biowest (Nuaille, France).
7
Compound Screening for Biological Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dimethyl sulfoxide (DMSO, 49), p-nitrosodimethylaniline, imidazole, nitroblue tetrazolium (NBT), quinine HCl (1), 4-methyl-7-ethoxycoumarin (3), 7-methoxycoumarin (5), 8-methoxypsoralen (6), acridine (7), diclofenac Na (11), hexachlorophene (16), indomethacin (19), ketoprofen ( 21), methyl β-naphthyl ketone (22), piroxicam (29), promethazine HCl (31), sulfanilamide (34), tetracycline HCl (35), 1,3-butylene glycol (47), 2-propanol (48), ethanol (50), and glycerin (51) were obtained from Fujifilm-Wako Pure Chemical Industries. Sulisobenzone (2), bithionol (9), doxycycline HCl (12), enoxacin (13), furosemide ( 14), glibenclamide (15), hydrochlorothiazide (16), ibuprofen (18), isoniazid (20), mequitazine (24), nalidixic acid (25), octyl dimethyl PABA (26), ofloxacin ( 27), omadine Na (28), prochlorperazine maleate (30), pyridoxine HCl (32), sparfloxacin (33), lactic acid (52), and penicillin G (54) were purchased from Sigma-Aldrich Japan. Benzophenone (8), lauric acid (53), and propylene glycol (55) were obtained from Junsei Chemical Co. (Tokyo, Japan), and 6-methylcoumarin (4) was purchased from Nacalai Tesque. Chlorpromazine HCl (10) and methyl-N-methylanthranilate (23) were purchased from Tokyo Chemical Industry (Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!